Ano5 (NM_177694) Mouse Untagged Clone

CAT#: MC222559

Ano5 (untagged) - Mouse anoctamin 5 (Ano5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ano5
• Synonyms: 9330162L24; Gdd1; Tmem16e
• Vector: pCMV6-Entry2
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC222559 representing NM_177694
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGAGCAGGAAGGCTTAACAGCCAAAGAAATAGACTATGCTTTCCAACAGAATGAGAACCTGGGCT
  CCAAAGAGACCAGCTTTCTCATCCCTGAAGACTTACAGTCACCGCCTGAGAAGCGATTCAATTTGTTCCT
  GAGGAGGCGTCTTATGTTCCAGAGAAGTGAGCACAGCAAAGACTCGGTCTTCTTCAGAGATGGAATCCGG
  CAGATCGACTTTGTCCTGTCCTACGTGGAGGATTTGAAGAAAGATGGAGAGCTGAAGGCAGAAAGAAGAC
  GAGAGTTTGAACAAAATCTCAGAAAAACAGGTCTTGACTTGGAAACAGAAGACAAATTGAACTCTGAAGA
  TGGGAAAACCTATTTTGTGAAGATTCACGCCCCATGGGAAGTCCTGGTCACTTACGCTGAAGTGCTGGGA
  ATCAAGATGCCTATTAAGTTGAGTGACATCCCACGGCCAAAGTACCCACCCCTGTCTTACATGCTTGGGG
  CGGTGAAGCTTCCATCCTCTGTGAAGTATCCTACTCCTGAGTATTTCACTGCCCAGTTCAGCAGACACCG
  TCAAGAACTCTTCCTCATTGAAGATGAGGCCACTTTCTTTCCATCCTCAACAAGGAACAGAATTGTTTAC
  TATATTCTGTCACGATGCCCTTTTGGAGTGGAAGAGGGGAAGAAAAAGATTGGGATCGAGAGACTGCTGA
  ACTCGAACACTTATTTATCTGCCTACCCGCTCCATGATGGCCAGTATTGGAAACCATCAAAAACTACTCG
  CCCCAATGAAAGGTACAACCTTTGCAAGAACTGGGCTCGATTTTCCTACTTTTACAAGGAGCAACCTTTT
  CATTTGATTCGGAATTATTTTGGAGAAAAAATCGGTATCTATTTTGTATTTCTCGGGTATTACACAGAAA
  TGCTGTTGTTTGCGGCTCTGGTCGGCTTAGCCTGCTTCATCTATGGCTTGTTATCAATGGAAAATAACCG
  AACTAGCACTGAAATCTGTGACCCTGACATCGGAGGTCAGATGATCATGTGCCCACTTTGCGATGAAGTG
  TGTGATTACTGGAGACTGAACACCACGTGTTTGCATTCAAAGTTCTCCCATCTGTTTGATAACGAGTCGA
  CTGTGTTCTTTGCGCTCTTCATGGGGATCTGGGTCACATTGTTTCTGGAGTTTTGGAAGCAGCGACAAGC
  CAGGCTTGAGTACGAATGGGATCTGGTGGACTTTGAAGAAGAACAGCAGCAGCTCCAGCTTCGGCCTGAA
  TTTGAAGCTATGTGTAAACACAAGAAGATGAATCCTGTGACAAAGGAAATGGAGCCACACATGCCTCTAT
  GCCATCGCATCCCATGGTACTTTGTGTCAGGGACCACAGTGACCTTTGGGATGGCTCTCCTCCTCAGCAG
  CATGGTGTCCATCCTCATATACCGCCTGTCAGTCTTTGCCACTTTTGCCAGCTTCATGGAAAGCGAAGCC
  ACCCTGCAGAGCGTGAAGAGCTTCTTTACTCCACAGCTGGCTACGGCTCTCTCTGGATCATGCCTGAACT
  GCATTGTCATTTTGATACTGAATTTTTTTTATGAGAAGATATCTGCCTGGATTACGAAAATGGAAATACC
  ACGAACTCACCAGGAGTATGAGAGCAGCCTCACTCTGAAGATGTTTCTCTTCCAATTTGTGAACTATTAC
  TCATCTTGCTTCTATGTGGCCTTCTTTAAAGGGAAGTTCGTGGGATATCCTGGGAGTTACACATATATGT
  TCAACATATGGAGAAGCGAAGAGTGTGGTCCTGCAGGCTGTCTGATAGAGCTGACCACCCAGCTAACCAT
  CATTATGATTGGAAAACAGATTTTTGGAAACATCCATGAAGCCTTTCAACCCTTGATTTTTAATTGGTGG
  AGACGCCGAAGAGCCCGAACCCACTCTGAGAAACTGTACAGTCGTTGGGAGCAAGATCATGACCTTCAGG
  TTTATGGACATCGTGGGCTGTTTTATGAGTACTTGGAAACAGTTATCCAGTTTGGGTTTGCCACGCTGTT
  TGTGGCCTCTTTTCCTCTGGCACCCCTGTTTGCTCTCATGAACAACATCATGGGAATCCGAGTGGATGCC
  TGGAAGCTGACAACTCAGTACAGGAGACCTGTCGCAGCGAAAGCCCACTCCATAGGTGTCTGGCAAGACA
  TTCTTTTTGGAATGGCCATCGTTTCTGTTGCAACTAATGCCTTCATTGTCTCATTTACATCTGACATCAT
  CCCCCGGTTAGTTTACTTCTATGCCTACTCAACGAATTCCACAGAGCCCTTGAGTGGATACGTCAATAAC
  AGCCTGTCAGTGTTCCTGATAGCTGACTTTCCCAACCACACAGTGCCCATGGAGAAAAAAGACTTCGTCA
  CCTGCAGGTACAGAGATTATAGGTACCCTCCTGATCATGAGGATAAGTATAGCCATAACATGCAGTTCTG
  GCATGTTCTTGCTGCCAAGATGACCTTCATCATAGTGATGGAACACGTCGTGTTTCTGTTTAAATTTTTA
  TTAGCCTGGCTGATACCAGATGTTCCTAAAGATGTTGTGGAGAAAATCAAACGAGAAAAGTTAATGACTA
  TCAAAATTATTCATGACTTTGAGCTCAACAAGTTGAAAGAAAACTTGGATGTTGAATATGGTAACATCAT
  GAAGAATGTTTTAGTAGATGAAGACAATTCCCTGAAGGCTAAAACTACAGTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177694
• Insert Size: 2715 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177694.5, NP_808362.2
• RefSeq Size: 7783 bp
• RefSeq ORF: 2715 bp
• Locus ID: 233246
• UniProt ID: Q75UR0
• Cytogenetics: 7 B4
• Gene Summary: This gene encodes a member of the anoctamin family, which in mammals is comprised of 10 members. Anoctamin proteins are proposed to have eight transmembrane domains with both termini facing the cytoplasm and a C-terminal domain of unknown function. While some members have been characterized as calcium-activated chloride channels, this protein is reported to have little anion conductance activity. Elevated levels of this protein were found in dystrophic mice. In humans, mutations of this gene are associated with with musculoskeletal disorders such as myopathies, muscular dystrophy and gnathodiaphyseal dysplasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.