Nectin3 (NM_021497) Mouse Tagged ORF Clone

CAT#: MG223253

Pvrl3 (tGFP-tagged) - Mouse poliovirus receptor-related 3 (Pvrl3) transcript variant gamma, (10ug)

Product Images

Circular map for MG223253

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nectin3
• Synonyms: 2610301B19Rik; 3000002N23Rik; 4921513D19Rik; AA407785; AU016832; AW538082; CD113; Pvrl3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223253 representing NM_021497
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCGGACCCCGGGCCCGGCCCCGTTGTGTCCTGGAGGCGGCAAAGCACAACTTTCCTCGGCGTTTC
  CTCCCGCGGCCGGACTGCTGCTGCCGGCCCCGACGCCGCCGCCGCTGCTGCTGCTGCTTATTCCCCTGCT
  TCTCTTCTCCCGGCTCTGTGGTGCCTTAGCTGGATCAATTATTGTGGAGCCACATGTCACAGCAGTGTGG
  GGAAAGAATGTTTCATTGAAGTGTTTAATTGAAGTGAATGAAACTATAACCCAGATCTCATGGGAGAAGA
  TACATGGCAAAAGTACACAGACTGTTGCAGTTCATCATCCTCAGTATGGATTCTCTGTTCAAGGAGATTA
  TCAGGGAAGAGTCTTGTTTAAAAACTATTCACTTAATGATGCAACAATTACTCTGCATAACATAGGCTTC
  TCAGATTCTGGAAAATATATATGCAAAGCCGTTACATTCCCACTTGGAAATGCTCAGTCCTCTACAACAG
  TGACTGTGTTAGTTGAACCCACAGTGAGCCTGATAAAAGGGCCGGATTCTTTAATTGATGGAGGGAATGA
  GACAGTAGCAGCCGTTTGTGTAGCAGCCACTGGAAAGCCAGTCGCACAGATTGACTGGGAAGGTGATCTT
  GGTGAAATGGAATCTAGTACAACTTCTTTTCCTAATGAAACAGCAACGATTGTCAGCCAATACAAGCTGT
  TTCCCACAAGATTTGCTCGAGGAAGGCGAATTACTTGTGTTGTAAAACATCCAGCCTTAGAAAAGGACAT
  TCGCTACTCTTTCATACTAGACATACAGTATGCTCCTGAAGTTTCAGTAACAGGATATGATGGAAATTGG
  TTCGTGGGAAGAAAAGGTGTTAACCTCAAGTGTAATGCTGATGCAAACCCTCCACCCTTCAAGTCCGTGT
  GGAGCAGGTTGGATGGACAATGGCCTGATGGTTTATTGGCGTCAGATAATACTCTTCATTTTGTCCATCC
  ATTGACTGTCAATTATTCTGGCGTTTATGTCTGTAAAGTATCAAATTCCCTTGGTCAAAGAAGTGATCAA
  AAGGTTATCTACATTTCAGACATCCCGCTTACGCAGACCTCATCCATAGCAGTGGCTGGAGCCGTGATTG
  GAGCTGTCCTGGCCCTCTTCATCATCACCGTCTTTGTGACTGTGTTGCTGACGCCTCGGAAAAAGAGACC
  GTCCTATCTTGACAAAGTAATCGACCTTCCACCTACACATAAGCCACCCCCTGTATATGAAGAACGAATT
  CCTTCTCTCCCTCAGAAAGACCTTCTTGGCCAGGTACGTGCTCTCGAAGACACT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223253 representing NM_021497
  Red=Cloning site Green=Tags(s)
  
  MARTPGPAPLCPGGGKAQLSSAFPPAAGLLLPAPTPPPLLLLLIPLLLFSRLCGALAGSIIVEPHVTAVW
  GKNVSLKCLIEVNETITQISWEKIHGKSTQTVAVHHPQYGFSVQGDYQGRVLFKNYSLNDATITLHNIGF
  SDSGKYICKAVTFPLGNAQSSTTVTVLVEPTVSLIKGPDSLIDGGNETVAAVCVAATGKPVAQIDWEGDL
  GEMESSTTSFPNETATIVSQYKLFPTRFARGRRITCVVKHPALEKDIRYSFILDIQYAPEVSVTGYDGNW
  FVGRKGVNLKCNADANPPPFKSVWSRLDGQWPDGLLASDNTLHFVHPLTVNYSGVYVCKVSNSLGQRSDQ
  KVIYISDIPLTQTSSIAVAGAVIGAVLALFIITVFVTVLLTPRKKRPSYLDKVIDLPPTHKPPPVYEERI
  PSLPQKDLLGQVRALEDT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021497
• ORF Size: 1314 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021497.2, NP_067472.1
• RefSeq Size: 1861 bp
• RefSeq ORF: 1317 bp
• Locus ID: 58998
• UniProt ID: Q9JLB9
• Cytogenetics: 16 B5
• Gene Summary: Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectins-like or other nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.[UniProtKB/Swiss-Prot Function]