Art3 (NM_181728) Mouse Tagged ORF Clone

CAT#: MG223250

Art3 (tGFP-tagged) - Mouse ADP-ribosyltransferase 3 (Art3), (10ug)

Product Images

Circular map for MG223250

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Art3
• Synonyms: 4930569O04Rik; ARTC3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223250 representing NM_181728
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATGGGACATTTTGAAATGGTCACCACGCTGCTGGCAGCCGCAGTTCTAATGGACATCTTCCAGG
  TGAAGGCGGAAGTGTTAGACATGGCAGAGAACGCGTTTGACGACGAATACCTGAAGTGCAAAAGCAGAAT
  GGAATCGAAGTATATTCCGCAGATGAAGAGGGAGGAGTGGGCAAACGATGCGCTCCTGCGGATGGTGTGG
  GACAATGCGGAGATCCAGTGGGAAGCGCGGAAGGCTCAGCTCTTCCTCCCTAGAAACTTCAAGGACACCT
  ATGGCATAGCTCTGACGGCCTACGTGAATGAGGCTCAAGAGCAGACTTCCTTCTACCACACATTCAGCAG
  CGCTGTGAAGATGGCCGGCCTGTCCCGAAGACGGTACATCTATAACTTCCCGTTCAAGGCTTTTCATTTT
  TACTTGGTGAGAGCCCTGCAGCTGCTGAGACGACCTTGCGAGAAAAGTTACAAAACTGTGGTGTACAGCA
  CAAGCCCAGACATTTCATTCACATTCGGAGAGCAAAACCAAGCCAGGCTGGGTAACTTCACGTTGGCGTA
  TTCAGCCAAACCGGAGACAGCCGACAACCAACGGGTGCTAACCATCCAGACCTGCTTTGGAGTCGCCGTG
  GGAAAGTTTCTTAATAAAGAAGACGACAGCGTTGTTTTAATACCTCTGAGTGAGGTTTTTCAAGTGTCGA
  GGAAAGGAACTAGCAACGACCTTGTCCTTCAGAGCATAAACAGTACCTGCAGCTACTACGAGTGCGCATT
  CCTCGGGGGTCTGAAAACAGAAAATTGTATCGCGAACGCAGAATATATTGACCCTAGGTATCTCTACAAC
  CCAGATATGGACAATCAGAAGCTTGAAGACTCTGGTAGGAACAACCTTGACCCTGACAGAATGCCAGAAA
  TTAAAGTGCTACAAACAGAGGAAAATCCTCTCCTGCCGGATGAAAAGCCTGCTCCAGGTCTGGTTCCAGG
  TCCGAAATCCCATCCCTCTGCATCCTCTGGCAATACGCTCCTTCCATCAGTCATGGCATCCACCATTTTA
  CTCGTTGCTTCTGCTGTAAACTTCATCGAGCTA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223250 representing NM_181728
  Red=Cloning site Green=Tags(s)
  
  MKMGHFEMVTTLLAAAVLMDIFQVKAEVLDMAENAFDDEYLKCKSRMESKYIPQMKREEWANDALLRMVW
  DNAEIQWEARKAQLFLPRNFKDTYGIALTAYVNEAQEQTSFYHTFSSAVKMAGLSRRRYIYNFPFKAFHF
  YLVRALQLLRRPCEKSYKTVVYSTSPDISFTFGEQNQARLGNFTLAYSAKPETADNQRVLTIQTCFGVAV
  GKFLNKEDDSVVLIPLSEVFQVSRKGTSNDLVLQSINSTCSYYECAFLGGLKTENCIANAEYIDPRYLYN
  PDMDNQKLEDSGRNNLDPDRMPEIKVLQTEENPLLPDEKPAPGLVPGPKSHPSASSGNTLLPSVMASTIL
  LVASAVNFIEL
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181728
• ORF Size: 1083 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181728.3, NP_859417.2
• RefSeq Size: 1578 bp
• RefSeq ORF: 1086 bp
• Locus ID: 109979
• UniProt ID: Q8R2G4
• Cytogenetics: 5 46.54 cM