Prp2 (NM_031499) Mouse Tagged ORF Clone

CAT#: MG223193

Prp2 (tGFP-tagged) - Mouse proline rich protein 2 (Prp2), (10ug). Note: ORF is codon optimized

Product Images

Circular map for MG223193

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prp2
• Synonyms: MP1; MP14
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223193 representing NM_031499, codon optimized.
  Due to the complexity of NM_031499, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGTGGTCCTTTTCACAGTAGCGCTGCTGGCACTCAGCTCCGCACAGGAGCCTAGAGAAGAACTTC
  AGAATCAGATTCAGATTCCTAATCAGCGCCCCCCTCCAAGCGGGAGCCAGCCGAGGCCGCCCGTTAACGG
  CTCCCAACAAGGACCTCCTCCACCAGGCGGACCTCAACCCCGTCCTCCACAAGGTCCTCCACCTCCAGGA
  GGTCCACAGCCAAGACCTCCACAGGGACCCCCTCCACCTGGGGGTCCACAACCACGTCCGCCTCAGGGTC
  CGCCTCCTCCAGGTGGTCCTCAGCCAAGGCCACCTCAGGGTCCCCCTCCTCCTGGAGGACCACAACCTCG
  GCCTCCTCAGGGTCCACCACCTCCTGGGGGTCCACAGCAACGGCCCCCTCAGGGTCCACCTCCACCAGGC
  GGTCCTCAACAGAGGCCTCCTCAGGGACCCCCACCTCCTGGTGGACCACAGCCTCGGCCACCACAGGGAC
  CCCCTCCTCCAGCAGGCCCTCAGCCAAGACCTCCTCAGGGCCCACCACCACCGGGCCCTCATCTGAGGCC
  AACTCAGGGGCCACCACCAACCGGTGGTCCACAGCAGAGGTATCCACAGTCCCCGCCACCGCCTGGAGGC
  CCTCAACCTAGGCCACCTCAAGGTCCTCCCCCTCCGGGAGGGCCACACCCTCGCCCCACCCAGGGTCCCC
  CGCCCACCGGACCCCAGCCGCGCCCAACTCAGGGCCCACCTCCCACCGGTGGCCCACAACAGCGACCACC
  ACAAGGCCCTCCACCACCGGGTGGACCACAGCCTAGGCCACCTCAGGGACCTCCGCCTCCTACCGGCCCT
  CAGCCTAGGCCTACACAAGGACCACACCCAACTGGAGGTCCCCAGCAGACCCCTCCACTTGCTGGAAACC
  CACAAGGACCTCCGCAGGGTAGGCCTCAGGGACCACAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223193 representing NM_031499
  Red=Cloning site Green=Tags(s)
  
  MLVVLFTVALLALSSAQEPREELQNQIQIPNQRPPPSGSQPRPPVNGSQQGPPPPGGPQPRPPQGPPPPG
  GPQPRPPQGPPPPGGPQPRPPQGPPPPGGPQPRPPQGPPPPGGPQPRPPQGPPPPGGPQQRPPQGPPPPG
  GPQQRPPQGPPPPGGPQPRPPQGPPPPAGPQPRPPQGPPPPGPHLRPTQGPPPTGGPQQRYPQSPPPPGG
  PQPRPPQGPPPPGGPHPRPTQGPPPTGPQPRPTQGPPPTGGPQQRPPQGPPPPGGPQPRPPQGPPPPTGP
  QPRPTQGPHPTGGPQQTPPLAGNPQGPPQGRPQGPQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031499
• ORF Size: 948 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031499.2, NP_113687.2
• RefSeq Size: 951 bp
• RefSeq ORF: 951 bp
• Locus ID: 83380
• Cytogenetics: 6 G1
• Gene Summary: This gene encodes a member of the heterogeneous family of proline-rich salivary glycoproteins. The expression of this gene is dramatically induced in the parotid and submandibular glands of mice by beta-adrenergic stimulation. The encoded protein serves an important dental function by protecting mice against toxic dietary polyphenols such as tannins and influence the visco-elastic properties of the mucus. This gene is located in a cluster of closely related salivary proline-rich proteins on chromosome 6. [provided by RefSeq, Nov 2015]