Mrgprd (NM_203490) Mouse Tagged ORF Clone

CAT#: MG223044

Mrgprd (tGFP-tagged) - Mouse MAS-related GPR member D (Mrgprd), (10ug)

Product Images

Circular map for MG223044

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mrgprd
• Synonyms: Gm499; Mgrd; MrgD; TGR7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG223044 representing NM_203490
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCCACTCTTGACAGCAGCCCAGCTCCAGGTCTGACCATCAGTCCCACCATGGACCTTGTGACCT
  GGATCTACTTTTCAGTGACATTCCTCGCCATGGCCACGTGTGTGGGGGGGATGGCAGGCAACTCATTGGT
  GATTTGGCTCCTGAGCTGCAACGGCATGCAGAGGTCTCCCTTCTGTGTCTATGTGCTCAACCTGGCGGTG
  GCTGACTTCCTCTTCTTATTCTGCATGGCCTCCATGCTCAGCCTGGAAACAGGGCCCCTGCTCATAGTCA
  ACATTTCTGCCAAAATCTATGAAGGGATGAGGAGAATCAAGTACTTTGCCTATACAGCAGGCCTGAGCCT
  GCTGACAGCCATCAGCACCCAGCGCTGCCTCTCCGTGCTTTTCCCCATCTGGTATAAGTGCCACCGGCCC
  CGGCACCTGTCATCAGTGGTATCTGGTGCACTCTGGGCACTGGCCTTCCTGATGAACTTCCTGGCTTCTT
  TCTTCTGCGTCCAATTCTGGCATCCCAACAAACACCAGTGCTTCAAGGTGGACATTGTTTTCAACAGTCT
  TATCCTGGGGATCTTCATGCCGGTCATGATCCTGACCAGCACCATCCTCTTCATCCGGGTGCGGAAGAAC
  AGCCTGATGCAGAGACGGCGGCCCCGGCGGCTGTACGTGGTCATCCTGACTTCCATCCTTGTCTTCCTCA
  CCTGTTCTCTGCCCTTGGGCATCAACTGGTTCTTACTCTACTGGGTGGATGTGAAACGGGATGTGAGGCT
  ACTTTATAGCTGCGTATCACGCTTCTCTTCATCTTTGAGCAGCAGTGCCAACCCGGTCATTTACTTCCTC
  GTGGGCAGCCAGAAGAGCCACCGGCTGCAGGAGTCCCTGGGTGCTGTGCTGGGGCGGGCACTGCGGGATG
  AGCCTGAGCCAGAGGGCAGAGAGACGCCATCCACGTGTACTAATGATGGGGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG223044 representing NM_203490
  Red=Cloning site Green=Tags(s)
  
  MNSTLDSSPAPGLTISPTMDLVTWIYFSVTFLAMATCVGGMAGNSLVIWLLSCNGMQRSPFCVYVLNLAV
  ADFLFLFCMASMLSLETGPLLIVNISAKIYEGMRRIKYFAYTAGLSLLTAISTQRCLSVLFPIWYKCHRP
  RHLSSVVSGALWALAFLMNFLASFFCVQFWHPNKHQCFKVDIVFNSLILGIFMPVMILTSTILFIRVRKN
  SLMQRRRPRRLYVVILTSILVFLTCSLPLGINWFLLYWVDVKRDVRLLYSCVSRFSSSLSSSANPVIYFL
  VGSQKSHRLQESLGAVLGRALRDEPEPEGRETPSTCTNDGV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_203490
• ORF Size: 963 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203490.3, NP_987075.1
• RefSeq Size: 3143 bp
• RefSeq ORF: 966 bp
• Locus ID: 211578
• UniProt ID: Q91ZB8
• Cytogenetics: 7 F5
• Gene Summary: May regulate nociceptor function and/or development, including the sensation or modulation of pain. Functions as a specific membrane receptor for beta-alanine. The receptor couples with G-protein G(q) and G(i) (By similarity).[UniProtKB/Swiss-Prot Function]