Tgm2 (NM_009373) Mouse Tagged ORF Clone

CAT#: MG222936

Tgm2 (tGFP-tagged) - Mouse transglutaminase 2 C polypeptide (Tgm2), (10ug)

Product Images

Circular map for MG222936

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tgm2
• Synonyms: G[a]h; TG2; TGase2; tTG; tTGas
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222936 representing NM_009373
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGGAGCTGCTCCTGGAGAGGTGTGATTTGGAGATTCAGGCCAATGGCCGTGACCACCACACGG
  CCGACCTATGCCAAGAGAAACTGGTGCTGCGTCGTGGTCAGCGCTTCCGGCTGACTCTGTACTTCGAGGG
  CCGTGGCTACGAGGCCAGCGTGGACAGCCTCACGTTCGGTGCTGTGACCGGCCCAGATCCCAGTGAAGAG
  GCAGGGACCAAGGCCCGCTTCTCACTGTCTGACAATGTGGAGGAGGGATCTTGGTCAGCCTCAGTGCTGG
  ACCAACAGGACAATGTCCTCTCGCTACAGCTCTGCACCCCAGCCAATGCTCCTATTGGCCTGTACCGTCT
  CAGCCTAGAGGCTTCTACTGGCTACCAGGGCTCCAGCTTTGTGCTGGGCCACTTCATCCTGCTCTACAAT
  GCCTGGTGCCCAGCCGATGATGTGTACCTAGACTCAGAGGAGGAGCGACGGGAATATGTCCTTACGCAAC
  AGGGCTTCATCTACCAAGGCTCTGTCAAGTTCATCAAGAGTGTGCCTTGGAACTTTGGGCAGTTCGAGGA
  TGGAATCCTGGATACCTGCCTGATGCTCTTGGATATGAACCCCAAGTTCCTGAAGAACCGTAGTCGGGAC
  TGCTCACGCCGCAGCAGTCCCATCTATGTGGGCCGCGTGGTGAGCGCCATGGTCAACTGCAATGATGACC
  AGGGTGTGCTTCTGGGCCGCTGGGACAACAACTATGGGGATGGTATCAGTCCCATGGCCTGGATTGGCAG
  TGTGGACATTCTGCGGCGCTGGAAGGAACACGGCTGTCAGCAAGTGAAGTACGGGCAGTGCTGGGTGTTT
  GCAGCGGTGGCCTGCACAGTGCTGCGGTGCCTCGGCATCCCTACCAGAGTGGTGACCAACTACAACTCCG
  CCCACGACCAGAACAGCAACCTGCTCATCGAGTACTTCCGAAATGAGTTCGGGGAGCTGGAGAGCAACAA
  GAGCGAGATGATCTGGAACTTCCACTGCTGGGTGGAGTCCTGGATGACCAGGCCAGACCTACAGCCGGGC
  TATGAGGGGTGGCAGGCCATTGACCCCACACCACAGGAGAAGAGCGAAGGGACATACTGTTGTGGCCCAG
  TCTCAGTGCGAGCCATCAAGGAGGGAGACCTGAGTACCAAGTATGATGCACCCTTCGTGTTTGCCGAGGT
  CAACGCTGATGTGGTGGACTGGATCCGGCAGGAAGATGGGTCTGTGCTCAAATCCATCAACCGTTCCTTG
  GTCGTGGGGCAGAAGATCAGCACTAAGAGTGTGGGCCGTGATGACCGGGAGGACATCACCCATACCTACA
  AGTACCCAGAGGGGTCACCCGAGGAGAGGGAAGTCTTCACCAAGGCCAACCACCTGAACAAACTGGCAGA
  GAAAGAGGAGACAGGGGTGGCCATGCGCATCCGAGTGGGGGACAGTATGAGCATGGGCAACGACTTCGAC
  GTGTTTGCCCACATCGGCAACGACACCTCGGAGACTCGAGAGTGTCGTCTCCTGCTCTGTGCCCGCACTG
  TCAGCTACAACGGGGTGCTGGGGCCCGAGTGTGGCACTGAGGACATCAACCTGACCCTGGATCCCTACTC
  TGAGAACAGCATCCCACTTCGAATCCTCTACGAGAAGTACAGCGGGTGCCTGACAGAGTCAAACCTCATC
  AAGGTGCGGGGCCTTCTCATCGAACCAGCTGCCAACAGCTACCTGCTGGCTGAGAGAGATCTCTACCTGG
  AGAATCCCGAAATCAAGATCCGGGTCCTGGGAGAACCCAAGCAAAACCGCAAACTGGTGGCTGAGGTGTC
  CCTGAAGAACCCACTTTCCGATCCCCTGTATGACTGCATCTTCACTGTGGAGGGGGCTGGCCTGACCAAG
  GAGCAGAAGTCTGTGGAAGTCTCAGACCCGGTGCCAGCGGGCGATTTGGTCAAGGCACGGGTCGACCTGT
  TCCCGACTGATATTGGCCTCCACAAGCTGGTGGTGAACTTCCAGTGTGACAAGCTGAAGTCGGTGAAGGG
  TTACCGGAATGTTATCATCGGCCCGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222936 representing NM_009373
  Red=Cloning site Green=Tags(s)
  
  MAEELLLERCDLEIQANGRDHHTADLCQEKLVLRRGQRFRLTLYFEGRGYEASVDSLTFGAVTGPDPSEE
  AGTKARFSLSDNVEEGSWSASVLDQQDNVLSLQLCTPANAPIGLYRLSLEASTGYQGSSFVLGHFILLYN
  AWCPADDVYLDSEEERREYVLTQQGFIYQGSVKFIKSVPWNFGQFEDGILDTCLMLLDMNPKFLKNRSRD
  CSRRSSPIYVGRVVSAMVNCNDDQGVLLGRWDNNYGDGISPMAWIGSVDILRRWKEHGCQQVKYGQCWVF
  AAVACTVLRCLGIPTRVVTNYNSAHDQNSNLLIEYFRNEFGELESNKSEMIWNFHCWVESWMTRPDLQPG
  YEGWQAIDPTPQEKSEGTYCCGPVSVRAIKEGDLSTKYDAPFVFAEVNADVVDWIRQEDGSVLKSINRSL
  VVGQKISTKSVGRDDREDITHTYKYPEGSPEEREVFTKANHLNKLAEKEETGVAMRIRVGDSMSMGNDFD
  VFAHIGNDTSETRECRLLLCARTVSYNGVLGPECGTEDINLTLDPYSENSIPLRILYEKYSGCLTESNLI
  KVRGLLIEPAANSYLLAERDLYLENPEIKIRVLGEPKQNRKLVAEVSLKNPLSDPLYDCIFTVEGAGLTK
  EQKSVEVSDPVPAGDLVKARVDLFPTDIGLHKLVVNFQCDKLKSVKGYRNVIIGPA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009373
• ORF Size: 2058 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009373.3, NP_033399.1
• RefSeq Size: 3549 bp
• RefSeq ORF: 2061 bp
• Locus ID: 21817
• UniProt ID: P21981
• Cytogenetics: 2 78.72 cM
• Gene Summary: Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.[UniProtKB/Swiss-Prot Function]