Krt18 (NM_010664) Mouse Tagged ORF Clone

CAT#: MG222836

Krt18 (tGFP-tagged) - Mouse keratin 18 (Krt18), (10ug)

Product Images

Circular map for MG222836

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Krt18
• Synonyms: CK18; K18; Krt1-18
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222836 representing NM_010664
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTCACAACTCGCTCCACCACCTTCTCCACCAACTACCGGTCCCTGGGCTCTGTGCGAACTCCCA
  GCCAGCGGGTCCGGCCTGCCAGCAGCGCAGCCAGCGTCTATGCAGGTGCTGGGGGCTCCGGGTCCCGGAT
  ATCCGTGTCCCGCTCTGTCTGGGGTGGCTCTGTGGGGTCCGCAGGCCTGGCGGGAATGGGTGGAATCCAG
  ACCGAGAAGGAGACCATGCAAGACCTGAACGATCGCCTGGCCAGCTACCTAGACAAGGTGAAGAGCCTGG
  AAACTGAGAACAGGAGACTGGAGAGCAAAATCCGGGAACATCTGGAGAAGAAGGGGCCCCAGGGCGTCAG
  AGACTGGGGCCACTACTTCAAGATCATCGAAGACCTGAGGGCTCAGATCTTTGCGAATTCTGTGGACAAT
  GCCCGCATCGTCTTGCAGATCGACAATGCCCGCCTTGCCGCCGATGACTTTAGAGTCAAGTATGAGACAG
  AACTAGCCATGCGCCAGTCTGTGGAGAGCGACATCCATGGACTCCGCAAGGTGGTAGATGACACCAACAT
  CACAAGGCTGCAGCTGGAGACAGAAATCGAGGCACTCAAGGAAGAACTTCTGTTCATGAAGAAGAATCAT
  GAAGAGGAAGTCCAAGGTCTGGAAGCCCAGATTGCCAGCTCTGGATTGACTGTGGAAGTGGATGCCCCCA
  AATCTCAGGACCTCAGCAAGATCATGGCGGACATCCGCGCCCAGTATGAAGCGCTGGCTCAGAAGAACCG
  CGAGGAACTGGACAAGTACTGGTCTCAGCAGATTGAGGAGAGTACCACAGTTGTCACCACCAAGTCTGCC
  GAAATCAGGGACGCTGAGACCACACTCACGGAGCTGAGACGCACCCTCCAGACCTTGGAGATTGACTTGG
  ACTCCATGAAAAACCAGAACATCAACTTGGAGAACAGCCTCGGGGATGTGGAGGCCCGATACAAGGCACA
  GATGGAGCAGCTCAATGGGGTCCTTCTGCATCTGGAGTCAGAGCTGGCACAAACTCGGGCAGAGGGCCAG
  CGCCAGGCCCAGGAATATGAAGCCCTCTTGAACATCAAGGTGAAGCTTGAGGCAGAGATTGCCACCTACC
  GCCGCTTGCTGGAGGATGGAGAAGATTTCAGTCTCAACGATGCCCTGGACTCCAGCAACTCCATGCAAAC
  TGTGCAGAAGACAACTACCCGTAAGATCGTGGATGGCAGAGTGGTGTCCGAGACTAATGACACCAGAGTT
  CTGAGGCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222836 representing NM_010664
  Red=Cloning site Green=Tags(s)
  
  MSFTTRSTTFSTNYRSLGSVRTPSQRVRPASSAASVYAGAGGSGSRISVSRSVWGGSVGSAGLAGMGGIQ
  TEKETMQDLNDRLASYLDKVKSLETENRRLESKIREHLEKKGPQGVRDWGHYFKIIEDLRAQIFANSVDN
  ARIVLQIDNARLAADDFRVKYETELAMRQSVESDIHGLRKVVDDTNITRLQLETEIEALKEELLFMKKNH
  EEEVQGLEAQIASSGLTVEVDAPKSQDLSKIMADIRAQYEALAQKNREELDKYWSQQIEESTTVVTTKSA
  EIRDAETTLTELRRTLQTLEIDLDSMKNQNINLENSLGDVEARYKAQMEQLNGVLLHLESELAQTRAEGQ
  RQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFSLNDALDSSNSMQTVQKTTTRKIVDGRVVSETNDTRV
  LRH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010664
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010664.2, NP_034794.2
• RefSeq Size: 1400 bp
• RefSeq ORF: 1272 bp
• Locus ID: 16668
• UniProt ID: P05784
• Cytogenetics: 15 57.22 cM
• Gene Summary: When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.[UniProtKB/Swiss-Prot Function]