Tmprss15 (NM_178855) Mouse Tagged ORF Clone

CAT#: MG222784

Tmprss15 (tGFP-tagged) - Mouse transmembrane protease serine 15 (Tmprss15) transcript variant 2, (10ug)

Product Images

Circular map for MG222784

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tmprss15
• Synonyms: Entk; P; Prss7
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222784 representing NM_178855
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATCCAGCAGAGACGAGGCTGTAGGACATCACTCTATCAGCTCTTTTGAAGTGATGCTCTCAGCTC
  TCTTCATTATGCTCATGGTCTTCTCTATTGGATTAATTGCAGTGTCCTGGCTTGCAGTCAAGGAATCTGA
  AGGAGATGCAGCCCTGGGGAAAAGTCATGAAGTCAGAGGGACATTTAAAATAACATCTGGAGTGACATAT
  AATCCTAATTTACAAGACAAACACTCTGTGGATTTCAAAGTTCTTGCTTTTGACCTTCAGCAAATGATAG
  ACGAGATCTTTGAATCAAGTAGTCTGAAGAATGAATATGAAAAATCGAAGGTTTTTCAATTTGAAAAAGG
  CAGCGTTATTGTCCTCTTTGACCTCTTTTTTGCTCAGTGGGTGTCAGATAAAAATGTAAAAGAAGAACTG
  ATTCAGGGCATTGAAGCAAATATATCCAGCCAACTGGTCACTCTCCATATTGATTTGAACAGCATTGATA
  TCACAGCATCCTTGAGTGACTTCACAACAGCAGTCCCTGTAACAACTTCAGATAAGCTAACAACCAGCAG
  TCCTATGACAACTTCAGCATCACTGGGTAACTTATCAACAACAGTGGCTGCAACAACTTCAGGCCATGTC
  TCAATAGAGTGCCAGCCTGGTTCACGGCCCTGTGCTCATGCTTGGAACTGTGTAGCAACTGATTTGTTTT
  GTGATGGAGAAGTGAACTGTCCAGATGGTTCAGATGAAGACACTGGGCTATGTGCCACAGCTTGTGATGG
  AAGATTTTTGTTGACTGGAGATTCTGGGGTTTTCCAAGCTGATCGTTATCCAAGGCCAGATGAATCAGGT
  GTTGTTTGCCGATGGATCATCCGTGTAAACCAAGGACTTTCCATTAGAATGAACTTTGGATCTTTTATTC
  CACATTATACAGATGTGTTAGATATCTATGAAGGTATAGGACCTAGCAAAATTTTAAGAGGCTCCTTTTG
  GGAAACTGATCCTGGAACAATTCGAATTTTTTCCAACCTAGTTACTGTCACCTTTCTGATAAAATCAGAT
  GAATATGATTACATTGGTTTTAATGCAACGTACAGCACTTTCAACAACAGCGAACTTAACAATTATGAGA
  AAATCGACTGTACATTTGATGATGGATTTTGCTTCTGGACCCAGGATCTTGATGATGATAATGAGTGGGA
  AAGAATCCAAGTGACCACGTTTCCTTGCTACACTGGCCCACGTTTTGACCACACATATGGGAATGGCTCA
  GGATTTTACATTTCTACACCAACTGAACAGGGATGGAGAAGCGAAAGAGTAGGCCTTTCAAGCCTCTCTT
  TAGACCTCACTTCAGAGCCTGTCTGCCTTCATTTTTGGTATTACATGTGCTGTGAAAATGTCTACAATTT
  AAACATTCATATCAGCAGTGCTGAAACCACAGACAAGATAGTTTTTCAAAGGAAAGGAAACTATGGGAGG
  AACTGGAATTATGGTCAAGTCACATTAAATGAAACAGGAGAATTTAAGGTTGTGTTCAATGCTTTCAGAA
  ACCGAGGTTGCAGTACAATAGCCCTGGATGATATAAGCCTAACAAATGGAATTTGTAGTCAGAGTCCTTA
  TCCAGAACCAACCTTGGTTCCCACCCCTCCACCAGAGCTTCCAACTGACTGCGGAGGACCTTTTGAACTA
  TGGGAGCCAAATTCAACCTTCAGTTCTCCGAACTTTCCAGACAAATATCCCAATCAAGCTTCCTGTATAT
  GGAATTTAAATGCACAGAGAGGGAAAAATATTCAACTTCATTTTCAAGAATTCGATTTAGAAAACATCAA
  TGATGTGGTGGAAGTTAGAGATGGGGGAGAATTTGACTCCCTGCTCCTAGCTGTGTACACAGGACCTGGT
  CCTGTGAAGGACTTGTTCTCTACCACCAATAGGATGACTGTGATTTTCACCACAAACATGGAGACCAGAC
  GCAAGGGCTTCAAAGCAAATTTCACTTCCGGCTATTACTTGGGGATTCCAGAGCCCTGCCAAGATGACGA
  ATTTCAGTGCAAAGATGGAAACTGCATTCCTCTGGGGAATCTCTGTGATAGCTACCCGCACTGTAGAGAT
  GGCTCAGATGAAGCAAGCTGTGTGCGTTTTCTTAATGGCACAAGAAGCAACAATGGGTTGGTACAGTTCA
  ATATCCATAGCATATGGCATATAGCTTGTGCTGAGAACTGGACCACACAAATTTCAAATGAAGTTTGTCA
  TCTTCTGGGACTAGGGAGTGCAAACTCATCAATGCCAATATCATCTACCGGAGGTGGACCATTTGTGAGA
  GTAAACCAAGCCCCTAATGGAAGCTTGATACTAACTCCTAGTCTACAATGCTCCCAAGATTCCTTGATTC
  TATTGCAATGCAACCATAAATCATGTGGAGAAAAAAAGGTGACTCAGAAAGTCAGCCCAAAGATCGTTGG
  TGGAAGTGACGCCCAGGCAGGGGCCTGGCCCTGGGTTGTGGCTTTGTATCACAGAGACAGGTCTACAGAC
  CGTCTGCTCTGCGGTGCATCTCTTGTCAGCAGTGACTGGCTGGTGTCAGCTGCACACTGTGTGTATAGAA
  GAAATCTAGATCCAACCAGATGGACAGCAGTCCTGGGCCTGCATATGCAATCAAATCTGACTTCTCCTCA
  AGTAGTAAGACGGGTGGTTGATCAAATTGTCATAAACCCTCATTATGACAGACGCAGAAAGGTCAACGAC
  ATCGCTATGATGCACCTTGAGTTTAAAGTAAATTACACAGATTATATACAGCCTATTTGTTTACCAGAAG
  AGAATCAAATATTTATTCCAGGAAGAACTTGTTCGATTGCTGGCTGGGGATATGATAAAATAAATGCAGG
  TTCTACTGTTGATGTGCTGAAAGAAGCTGATGTTCCTCTTATATCCAATGAAAAGTGCCAGCAACAGCTG
  CCAGAATACAACATCACTGAAAGTATGATATGTGCAGGATATGAAGAAGGAGGAATAGATTCTTGTCAGG
  GTGATTCTGGAGGGCCATTAATGTGCCAAGAAAACAACAGATGGTTTCTTGTTGGTGTGACATCATTTGG
  AGTCCAGTGTGCACTGCCTAATCACCCTGGGGTATATGTCCGAGTCTCACAGTTTATAGAATGGATCCAT
  AGCTTTCTACAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222784 representing NM_178855
  Red=Cloning site Green=Tags(s)
  
  MKSSRDEAVGHHSISSFEVMLSALFIMLMVFSIGLIAVSWLAVKESEGDAALGKSHEVRGTFKITSGVTY
  NPNLQDKHSVDFKVLAFDLQQMIDEIFESSSLKNEYEKSKVFQFEKGSVIVLFDLFFAQWVSDKNVKEEL
  IQGIEANISSQLVTLHIDLNSIDITASLSDFTTAVPVTTSDKLTTSSPMTTSASLGNLSTTVAATTSGHV
  SIECQPGSRPCAHAWNCVATDLFCDGEVNCPDGSDEDTGLCATACDGRFLLTGDSGVFQADRYPRPDESG
  VVCRWIIRVNQGLSIRMNFGSFIPHYTDVLDIYEGIGPSKILRGSFWETDPGTIRIFSNLVTVTFLIKSD
  EYDYIGFNATYSTFNNSELNNYEKIDCTFDDGFCFWTQDLDDDNEWERIQVTTFPCYTGPRFDHTYGNGS
  GFYISTPTEQGWRSERVGLSSLSLDLTSEPVCLHFWYYMCCENVYNLNIHISSAETTDKIVFQRKGNYGR
  NWNYGQVTLNETGEFKVVFNAFRNRGCSTIALDDISLTNGICSQSPYPEPTLVPTPPPELPTDCGGPFEL
  WEPNSTFSSPNFPDKYPNQASCIWNLNAQRGKNIQLHFQEFDLENINDVVEVRDGGEFDSLLLAVYTGPG
  PVKDLFSTTNRMTVIFTTNMETRRKGFKANFTSGYYLGIPEPCQDDEFQCKDGNCIPLGNLCDSYPHCRD
  GSDEASCVRFLNGTRSNNGLVQFNIHSIWHIACAENWTTQISNEVCHLLGLGSANSSMPISSTGGGPFVR
  VNQAPNGSLILTPSLQCSQDSLILLQCNHKSCGEKKVTQKVSPKIVGGSDAQAGAWPWVVALYHRDRSTD
  RLLCGASLVSSDWLVSAAHCVYRRNLDPTRWTAVLGLHMQSNLTSPQVVRRVVDQIVINPHYDRRRKVND
  IAMMHLEFKVNYTDYIQPICLPEENQIFIPGRTCSIAGWGYDKINAGSTVDVLKEADVPLISNEKCQQQL
  PEYNITESMICAGYEEGGIDSCQGDSGGPLMCQENNRWFLVGVTSFGVQCALPNHPGVYVRVSQFIEWIH
  SFLH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178855
• ORF Size: 3162 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178855.4, NP_849186.2
• RefSeq Size: 3681 bp
• RefSeq ORF: 3165 bp
• Locus ID: 19146
• UniProt ID: P97435
• Cytogenetics: 16 C3.1-C3.2
• Gene Summary: This gene encodes an enzyme that proteolytically activates the pancreatic proenzyme trypsinogen, converting it into trypsin. The encoded protein is cleaved into two chains that form a heterodimer linked by a disulfide bond. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]