Tmprss15 (NM_178855) Mouse Untagged Clone

CAT#: MC223392

Tmprss15 (untagged) - Mouse transmembrane protease, serine 15 (Tmprss15), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tmprss15
• Synonyms: Entk; P; Prss7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC223392 representing NM_178855
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATCCAGCAGAGACGAGGCTGTAGGACATCACTCTATCAGCTCTTTTGAAGTGATGCTCTCAGCTC
  TCTTCATTATGCTCATGGTCTTCTCTATTGGATTAATTGCAGTGTCCTGGCTTGCAGTCAAGGAATCTGA
  AGGAGATGCAGCCCTGGGGAAAAGTCATGAAGTCAGAGGGACATTTAAAATAACATCTGGAGTGACATAT
  AATCCTAATTTACAAGACAAACACTCTGTGGATTTCAAAGTTCTTGCTTTTGACCTTCAGCAAATGATAG
  ACGAGATCTTTGAATCAAGTAGTCTGAAGAATGAATATGAAAAATCGAAGGTTTTTCAATTTGAAAAAGG
  CAGCGTTATTGTCCTCTTTGACCTCTTTTTTGCTCAGTGGGTGTCAGATAAAAATGTAAAAGAAGAACTG
  ATTCAGGGCATTGAAGCAAATATATCCAGCCAACTGGTCACTCTCCATATTGATTTGAACAGCATTGATA
  TCACAGCATCCTTGAGTGACTTCACAACAGCAGTCCCTGTAACAACTTCAGATAAGCTAACAACCAGCAG
  TCCTATGACAACTTCAGCATCACTGGGTAACTTATCAACAACAGTGGCTGCAACAACTTCAGGCCATGTC
  TCAATAGAGTGCCAGCCTGGTTCACGGCCCTGTGCTCATGCTTGGAACTGTGTAGCAACTGATTTGTTTT
  GTGATGGAGAAGTGAACTGTCCAGATGGTTCAGATGAAGACACTGGGCTATGTGCCACAGCTTGTGATGG
  AAGATTTTTGTTGACTGGAGATTCTGGGGTTTTCCAAGCTGATCGTTATCCAAGGCCAGATGAATCAGGT
  GTTGTTTGCCGATGGATCATCCGTGTAAACCAAGGACTTTCCATTAGAATGAACTTTGGATCTTTTATTC
  CACATTATACAGATGTGTTAGATATCTATGAAGGTATAGGACCTAGCAAAATTTTAAGAGGCTCCTTTTG
  GGAAACTGATCCTGGAACAATTCGAATTTTTTCCAACCTAGTTACTGTCACCTTTCTGATAAAATCAGAT
  GAATATGATTACATTGGTTTTAATGCAACGTACAGCACTTTCAACAACAGCGAACTTAACAATTATGAGA
  AAATCGACTGTACATTTGATGATGGATTTTGCTTCTGGACCCAGGATCTTGATGATGATAATGAGTGGGA
  AAGAATCCAAGTGACCACGTTTCCTTGCTACACTGGCCCACGTTTTGACCACACATATGGGAATGGCTCA
  GGATTTTACATTTCTACACCAACTGAACAGGGATGGAGAAGCGAAAGAGTAGGCCTTTCAAGCCTCTCTT
  TAGACCTCACTTCAGAGCCTGTCTGCCTTCATTTTTGGTATTACATGTGCTGTGAAAATGTCTACAATTT
  AAACATTCATATCAGCAGTGCTGAAACCACAGACAAGATAGTTTTTCAAAGGAAAGGAAACTATGGGAGG
  AACTGGAATTATGGTCAAGTCACATTAAATGAAACAGGAGAATTTAAGGTTGTGTTCAATGCTTTCAGAA
  ACCGAGGTTGCAGTACAATAGCCCTGGATGATATAAGCCTAACAAATGGAATTTGTAGTCAGAGTCCTTA
  TCCAGAACCAACCTTGGTTCCCACCCCTCCACCAGAGCTTCCAACTGACTGCGGAGGACCTTTTGAACTA
  TGGGAGCCAAATTCAACCTTCAGTTCTCCGAACTTTCCAGACAAATATCCCAATCAAGCTTCCTGTATAT
  GGAATTTAAATGCACAGAGAGGGAAAAATATTCAACTTCATTTTCAAGAATTCGATTTAGAAAACATCAA
  TGATGTGGTGGAAGTTAGAGATGGGGGAGAATTTGACTCCCTGCTCCTAGCTGTGTACACAGGACCTGGT
  CCTGTGAAGGACTTGTTCTCTACCACCAATAGGATGACTGTGATTTTCACCACAAACATGGAGACCAGAC
  GCAAGGGCTTCAAAGCAAATTTCACTTCCGGCTATTACTTGGGGATTCCAGAGCCCTGCCAAGATGACGA
  ATTTCAGTGCAAAGATGGAAACTGCATTCCTCTGGGGAATCTCTGTGATAGCTACCCGCACTGTAGAGAT
  GGCTCAGATGAAGCAAGCTGTGTGCGTTTTCTTAATGGCACAAGAAGCAACAATGGGTTGGTACAGTTCA
  ATATCCATAGCATATGGCATATAGCTTGTGCTGAGAACTGGACCACACAAATTTCAAATGAAGTTTGTCA
  TCTTCTGGGACTAGGGAGTGCAAACTCATCAATGCCAATATCATCTACCGGAGGTGGACCATTTGTGAGA
  GTAAACCAAGCCCCTAATGGAAGCTTGATACTAACTCCTAGTCTACAATGCTCCCAAGATTCCTTGATTC
  TATTGCAATGCAACCATAAATCATGTGGAGAAAAAAAGGTGACTCAGAAAGTCAGCCCAAAGATCGTTGG
  TGGAAGTGACGCCCAGGCAGGGGCCTGGCCCTGGGTTGTGGCTTTGTATCACAGAGACAGGTCTACAGAC
  CGTCTGCTCTGCGGTGCATCTCTTGTCAGCAGTGACTGGCTGGTGTCAGCTGCACACTGTGTGTATAGAA
  GAAATCTAGATCCAACCAGATGGACAGCAGTCCTGGGCCTGCATATGCAATCAAATCTGACTTCTCCTCA
  AGTAGTAAGACGGGTGGTTGATCAAATTGTCATAAACCCTCATTATGACAGACGCAGAAAGGTCAACGAC
  ATCGCTATGATGCACCTTGAGTTTAAAGTAAATTACACAGATTATATACAGCCTATTTGTTTACCAGAAG
  AGAATCAAATATTTATTCCAGGAAGAACTTGTTCGATTGCTGGCTGGGGATATGATAAAATAAATGCAGG
  TTCTACTGTTGATGTGCTGAAAGAAGCTGATGTTCCTCTTATATCCAATGAAAAGTGCCAGCAACAGCTG
  CCAGAATACAACATCACTGAAAGTATGATATGTGCAGGATATGAAGAAGGAGGAATAGATTCTTGTCAGG
  GTGATTCTGGAGGGCCATTAATGTGCCAAGAAAACAACAGATGGTTTCTTGTTGGTGTGACATCATTTGG
  AGTCCAGTGTGCACTGCCTAATCACCCTGGGGTATATGTCCGAGTCTCACAGTTTATAGAATGGATCCAT
  AGCTTTCTACATTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178855
• Insert Size: 3165 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178855.4, NP_849186.2
• RefSeq Size: 4149 bp
• RefSeq ORF: 3165 bp
• Locus ID: 19146
• UniProt ID: P97435
• Cytogenetics: 16 C3.1-C3.2
• Gene Summary: This gene encodes an enzyme that proteolytically activates the pancreatic proenzyme trypsinogen, converting it into trypsin. The encoded protein is cleaved into two chains that form a heterodimer linked by a disulfide bond. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.