Mapk8ip3 (NM_001163450) Mouse Tagged ORF Clone

CAT#: MG222727

Mapk8ip3 (tGFP-tagged) - Mouse mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3) transcript variant 5, (10ug)

Product Images

Circular map for MG222727

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mapk8ip3
• Synonyms: BB120594; D17Wsu15e; JIP-3; Jip3; JSAP1; JSAP1a; JSAP1b; JSAP1c; JSAP1d; mKIAA1066; Syd2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222727 representing NM_001163450
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGAGATCCAGATGGACGAGGGAGGAGGAGTGGTGGTGTACCAAGACGACTACTGCTCGGGCTCGG
  TCATGTCGGAGCGTGTGTCGGGCCTGGCGGGCTCCATCTACCGCGAGTTCGAGCGCCTCATTCACTGCTA
  TGACGAGGAGGTGGTCAAGGAGCTCATGCCGCTGGTGGTGAACGTGCTGGAGAACCTTGACTCGGTGCTG
  AGCGAGAACCAGGAGCACGAGGTGGAGCTGGAGCTCCTACGCGAGGACAACGAGCAGCTGCTCACGCAAT
  ACGAGCGCGAGAAGGCGCTGCGCAAACAGGCCGAGGAGAAATTCATCGAATTTGAAGATGCCTTGGAACA
  AGAGAAGAAAGAACTCCAGATCCAGGTAGAACATTATGAGTTTCAGACACGCCAGCTGGAGCTAAAGGCC
  AAAAACTATGCAGATCAGATTTCCCGACTGGAGGAACGAGAATCGGAGATGAAGAAGGAATACAATGCCC
  TGCACCAGCGGCACACAGAGATGATCCAGACCTATGTGGAACACATTGAAAGATCCAAGATGCAGCAAGT
  TGGGGGTAGCGGCCAAACAGAAAGCAGCCTGCCCGGGCGGAGCAGGAAGGAGCGTCCCACCTCTCTGAAT
  GTCTTCCCCCTGGCTGATGGCATGTGTCCAAATGATGAGATGTCTGAGTCAGGCCAGTCCTCAGCAGCTG
  CAACACCCAGTACCACAGGTACCAAGTCCAACACACCCACGTCCTCCGTGCCCTCAGCAGCAGTCACGCC
  ACTCAACGAGAGCCTACAGCCCCTGGGGGACTATGTCAGTGTCACAAAGAACAACAAGCAGGCCCGAGAG
  AAGCGCAATAGCCGTAACATGGAGGTCCAGGTCACCCAAGAGATGCGGAACGTCAGTATCGGCATGGGCA
  GCAGTGACGAGTGGTCCGATGTTCAGGACATTATCGACTCCACCCCAGAGCTGGATGTGTGTCCTGAAAC
  CCGTCTGGAGCGCACAGGAAGCAGCCCAACCCAGGGAATTGTAAACAAAGCTTTTGGAATCAACACTGAC
  TCCTTGTATCACGAACTCTCCACGGCGGGATCTGAGGTCATCGGGGATGTGGACGAGGGAGCTGATCTCC
  TAGGGGAGTTTTCAGTGCGCGATGATTTTTTTGGAATGGGCAAAGAAGTGGGGAACCTGCTGCTGGAGAA
  CTCACAGCTTCTAGAGACAAAAAATGCTTTAAATGTAGTGAAGAATGACCTCATTGCTAAGGTTGACCAA
  CTGTCAGGAGAACAGGAGGTCCTGAAGGGTGAGCTGGAAGCAGCCAAGCAAGCCAAAGTCAAGCTGGAGA
  ACCGAATCAAAGAGCTTGAAGAAGAACTGAAGAGAGTCAAGTCAGAGGCAGTAACTGCCCGCCGTGAGCC
  CAGAGAAGAGGTGGAGGATGTAAGCAGCTATCTCTGTACAGAATTGGACAAAATCCCCATGGCCCAGCGC
  CGACGCTTCACACGGGTGGAGATGGCCCGAGTGCTCATGGAACGCAACCAGTACAAGGAACGCCTCATGG
  AGCTGCAGGAGGCTGTGAGGTGGACTGAAATGATCAGAGCATCAAGGGAACACCCATCTGTCCAGGAGAA
  GAAGAAGTCCACCATCTGGCAGTTCTTTAGTCGCCTCTTCAGCTCCTCATCTAGCCCCCCTCCGGCCAAA
  CGATCCTACCCATCTGTGAACATTCACTACAAGTCACCCACTGCAGCTGGCTTTAGCCAGCGTCGCAGCC
  ATGCTTTGTGCCAGATCTCAGCCGGCAGCAGGCCCCTGGAGTTCTTCCCTGATGATGACTGCACCTCTTC
  TGCCCGGCGGGAGCAGAAGCGGGAGCAGTACCGCCAGGTTCGTGAACACGTGCGCAATGATGACGGGAGG
  CTGCAGGCCTGTGGGTGGAGCCTGCCTGCCAAGTACAAGCAGCTGAGCCCCAATGGAGGCCAGGAAGACA
  CCCGGATGAAAAATGTGCCTGTCCCTGTGTACTGCCGCCCTCTGGTGGAGAAGGACCCTTCGACAAAGCT
  GTGGTGTGCTGCTGGTGTCAACCTGAGTGGGTGGAAGCCACATGAAGAGGACTCTAGCAATGGACCCAAG
  CCTGTACCAGGTCGAGACCCTCTGACCTGTGACCGGGAAGGAGAAGGCGAACCCAAGAGCACACACCCAT
  CACCTGAGAAGAAGAAGGCAAAGGAAACCCCTGAGGCAGATGCTACCTCCAGTCGGGTATGGATCCTCAC
  CAGCACCCTGACAACCAGCAAGGTGGTGATCATTGATGCCAACCAGCCAGGCACAATTGTGGATCAGTTC
  ACAGTCTGCAATGCCCACGTCCTGTGTATCTCCAGCATTCCTGCGGCCAGTGACAGTGACTATCCCCCTG
  GGGAGATGTTCCTAGACAGTGATGTGAACCCTGAAGATTCAGGTGCTGATGGTGTGCTGGCTGGCATCAC
  CCTGGTGGGGTGTGCTACCCGCTGCAATGTTCCACGTAGCAACTGTTCCTCACGAGGAGACACCCCAGTA
  CTGGACAAGGGGCAGGGGGATGTGGCGACCACTGCCAATGGGAAGGTCAACCCGTCCCAATCCACAGAAG
  AAGCCACAGAAGCCACGGAGGTGCCAGACCCTGGTCCCAGCGAGTCAGAAGCAACGACAGTCCGGCCCGG
  GCCTCTCACAGAGCATGTCTTTACTGACCCAGCACCCACCCCATCCTCCAGCACCCAGCCTGCCAGTGAG
  AATGGGTCAGAGTCCAATGGCACCATTGTACAGCCTCAGGTGGAGCCCAGTGGGGAACTCTCAACAACAA
  CCAGTAGCGCTGCACCCACTATGTGGCTAGGAGCCCAGAATGGCTGGCTCTATGTGCATTCAGCGGTAGC
  CAACTGGAAGAAGTGTCTGCACTCCATCAAGCTAAAAGACTCTGTGCTGAGCCTGGTGCATGTCAAAGGC
  CGAGTGCTGGTAGCTCTTGCAGATGGGACCCTGGCTATCTTCCATCGTGGAGAGGATGGCCAGTGGGACC
  TGAGCAACTACCACCTAATGGACCTGGGCCACCCACACCACTCCATCCGCTGCATGGCTGTTGTGAATGA
  CCGAGTTTGGTGTGGCTACAAGAACAAGGTGCATGTTATCCAGCCCAAGACAATGCAGATTGAGAAATCA
  TTTGATGCCCACCCAAGGCGGGAAAGCCAGGTACGTCAGCTGGCCTGGATCGGTGATGGAGTGTGGGTCT
  CTATTCGCTTGGATTCTACCCTTCGGCTCTACCATGCTCACACCCACCAGCACCTGCAGGATGTGGACAT
  TGAGCCCTATGTTAGCAAGATGCTAGGAACCGGCAAGCTGGGCTTCTCCTTCGTGCGCATCACAGCCTTA
  CTCATTGCAGGCAACCGTCTGTGGGTGGGCACTGGCAATGGGGTTGTCATCTCCATCCCCTTGACTGAGA
  CTGTGGTCCTGCATCGAGGCCAGCTCCTAGGGCTCCGAGCCAACAAGACATCCCCAACATCTGGGGAGGG
  GACCCGCCCAGGGGGCATCATCCATGTGTATGGGGACGACAGCAGTGACAAGGCCGCCAGTAGTTTCATC
  CCCTACTGCTCCATGGCACAGGCTCAGCTTTGCTTCCATGGGCACCGTGATGCTGTCAAATTCTTTGTCT
  CTGTGCCAGGAAATGTGCTGGCCACTCTCAATGGCAGTGTGCTAGACAGCCCATCAGAGGGCCCTGGGCC
  TGCTGCACCCGCTGCAGATGCTGAGGGCCAGAAGTTGAAGAATGCACTGGTGCTGAGTGGTGGTGAAGGT
  TACATTGACTTCCGTATCGGAGACGGAGAGGATGATGAAACTGAGGAATGTGCCGGGGACGTGAACCAGA
  CAAAGCCCTCGTTGTCCAAGGCTGAGCGCAGCCACATCATCGTGTGGCAGGTGTCCTACACCCCTGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222727 representing NM_001163450
  Red=Cloning site Green=Tags(s)
  
  MMEIQMDEGGGVVVYQDDYCSGSVMSERVSGLAGSIYREFERLIHCYDEEVVKELMPLVVNVLENLDSVL
  SENQEHEVELELLREDNEQLLTQYEREKALRKQAEEKFIEFEDALEQEKKELQIQVEHYEFQTRQLELKA
  KNYADQISRLEERESEMKKEYNALHQRHTEMIQTYVEHIERSKMQQVGGSGQTESSLPGRSRKERPTSLN
  VFPLADGMCPNDEMSESGQSSAAATPSTTGTKSNTPTSSVPSAAVTPLNESLQPLGDYVSVTKNNKQARE
  KRNSRNMEVQVTQEMRNVSIGMGSSDEWSDVQDIIDSTPELDVCPETRLERTGSSPTQGIVNKAFGINTD
  SLYHELSTAGSEVIGDVDEGADLLGEFSVRDDFFGMGKEVGNLLLENSQLLETKNALNVVKNDLIAKVDQ
  LSGEQEVLKGELEAAKQAKVKLENRIKELEEELKRVKSEAVTARREPREEVEDVSSYLCTELDKIPMAQR
  RRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASREHPSVQEKKKSTIWQFFSRLFSSSSSPPPAK
  RSYPSVNIHYKSPTAAGFSQRRSHALCQISAGSRPLEFFPDDDCTSSARREQKREQYRQVREHVRNDDGR
  LQACGWSLPAKYKQLSPNGGQEDTRMKNVPVPVYCRPLVEKDPSTKLWCAAGVNLSGWKPHEEDSSNGPK
  PVPGRDPLTCDREGEGEPKSTHPSPEKKKAKETPEADATSSRVWILTSTLTTSKVVIIDANQPGTIVDQF
  TVCNAHVLCISSIPAASDSDYPPGEMFLDSDVNPEDSGADGVLAGITLVGCATRCNVPRSNCSSRGDTPV
  LDKGQGDVATTANGKVNPSQSTEEATEATEVPDPGPSESEATTVRPGPLTEHVFTDPAPTPSSSTQPASE
  NGSESNGTIVQPQVEPSGELSTTTSSAAPTMWLGAQNGWLYVHSAVANWKKCLHSIKLKDSVLSLVHVKG
  RVLVALADGTLAIFHRGEDGQWDLSNYHLMDLGHPHHSIRCMAVVNDRVWCGYKNKVHVIQPKTMQIEKS
  FDAHPRRESQVRQLAWIGDGVWVSIRLDSTLRLYHAHTHQHLQDVDIEPYVSKMLGTGKLGFSFVRITAL
  LIAGNRLWVGTGNGVVISIPLTETVVLHRGQLLGLRANKTSPTSGEGTRPGGIIHVYGDDSSDKAASSFI
  PYCSMAQAQLCFHGHRDAVKFFVSVPGNVLATLNGSVLDSPSEGPGPAAPAADAEGQKLKNALVLSGGEG
  YIDFRIGDGEDDETEECAGDVNQTKPSLSKAERSHIIVWQVSYTPE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163450
• ORF Size: 3918 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163450.1, NP_001156922.1
• RefSeq Size: 5486 bp
• RefSeq ORF: 3921 bp
• Locus ID: 30957
• Cytogenetics: 17 12.53 cM
• Gene Summary: The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (PubMed:10523642, PubMed:10629060). Promotes neuronal axon elongation in a kinesin- and JNK-dependent manner (PubMed:23576431, PubMed:25944905, PubMed:28259553). Activates cofilin at axon tips via local activation of JNK, thereby regulating filopodial dynamics and enhancing axon elongation (PubMed:23576431, PubMed:25944905, PubMed:28259553). Its binding to kinesin heavy chains (KHC), promotes kinesin-1 motility along microtubules and is essential for axon elongation and regeneration (PubMed:23576431, PubMed:25944905, PubMed:28259553). Regulates cortical neuronal migration by mediating NTRK2/TRKB anterograde axonal transport during brain development (PubMed:23576431, PubMed:25944905, PubMed:28259553). Acts as an adapter that bridges the interaction between NTRK2/TRKB and KLC1 and drives NTRK2/TRKB axonal but not dendritic anterograde transport, which is essential for subsequent BDNF-triggered signaling and filopodia formation (PubMed:23576431, PubMed:25944905, PubMed:28259553).[UniProtKB/Swiss-Prot Function]