Ltk (NM_206941) Mouse Tagged ORF Clone

CAT#: MG222631

Ltk (tGFP-tagged) - Mouse leukocyte tyrosine kinase (Ltk) transcript variant 2, (10ug)

Product Images

Circular map for MG222631

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ltk
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222631 representing NM_206941
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  CTGGAGACCCGCGCGGCGGCGCCGGGCAGCGGCGGGAGAGGAGGCGCGGCAGGTGGAGGGAGCGGCTGGA
  CGTCGCGAGCCCACTCTCCGCAGGCCGGACGCTCGCCGCGGGAAGGGGCCGAGGGCGGCGAGGGCTGCGC
  GGAGGCCTGGGCTGCGCTCCGCTGGGCTGCGGCGGGAGGCTTCGGGGGCGGCGGCGGGGCCTGCGCGGCG
  GGCGGCGGTGGCGGCGGCTACCGGGGCGGTGATACTTCTGAGTCTGACCTCCTCTGGGCTGATGGGGAAG
  ATGGCACATCCTTTGTCCACCCCAGTGGTGAGCTCTACCTACAGCCTCTGGCAGTCACAGAGGGCCATGG
  GGAGGTGGAGATCCGAAAGCATCCCAACTGCAGTCACTGCCCTTTCAAAGACTGCCAGTGGCAGGCAGAG
  CTCTGGACGGCCGAATGCACGTGCCCAGAGGGCACGGAGCTAGCTGTGGATAATGTCACTTGCATGGACC
  TGCCAACCACCGCAAGCCCTCTGATCCTGATGGGAGCTGTAGTGGCAGCCTTGGCACTGAGTCTCCTAAT
  GATGTGTGCAGTCCTGATTCTAGTGAACCAGAAGTGTCAGGGCCTGTGGGGGACCAGGCTGCCAGGCCCT
  GAGCTTGAGCTAAGCAAGCTTCGATCCTCTGCCATCAGGACAGCACCCAACCCTTACTATTGTCAGGTGG
  GACTCAGTCCTGCCCAGCCCTGGCCTTTGCCCCCAGGGCTCACTGAGGTTTCACCAGCCAATGTCACTCT
  ACTCAGAGCCCTTGGCCATGGTGCCTTTGGGGAAGTGTACGAGGGACTAGTGACTGGTCTTCCTGGGGAC
  TCCAGTCCTCTTCCAGTGGCTATTAAGACTCTGCCAGAGCTCTGCTCCCATCAGGATGAGCTGGATTTTC
  TCATGGAGGCTCTGATCATCAGCAAGTTCAGCCATCAGAACATTGTACGCTGTGTGGGGCTCAGCTTTCG
  GTCTGCCCCGCGCCTCATTCTGCTGGAGCTGATGTCTGGTGGGGACATGAAGAGCTTTTTGAGGCACAGC
  AGACCACACCCAGGACAACTGGCACCTCTGACCATGCAGGACCTATTGCAGCTGGCCCAGGATATAGCCC
  AGGGCTGCCACTACCTGGAGGAAAATCACTTCATTCACAGAGACATTGCTGCCCGTAACTGTCTGCTTAG
  CTGCAGTGGAGCCAGCCGAGTGGCCAAGATTGGAGATTTTGGAATGGCAAGAGATATCTACCAGGCCAGT
  TATTATCGCAAGGGTGGCCGGACCTTGCTCCCAGTCAAGTGGATGCCGCCAGAAGCTCTCCTGGAGGGCC
  TTTTCACATCCAAGACAGACTCCTGGTCTTTTGGGGTCCTGCTCTGGGAGATCTTCTCACTGGGGTATAT
  GCCCTACCCTGGACATACCAACCAGGAGGTTCTAGACTTCATTGCCACAGGGAACAGGATGGACCCTCCT
  AGGAACTGTCCTGGGCCAGTGTACCGAATCATGACCCAGTGTTGGCAGCATCAGCCGGAGCTCCGCCCTG
  ACTTTGGCAGCATCTTGGAACGGATTCAGTACTGCACTCAGGACCCTGATGTGCTGAACTCACCCCTGCC
  CGTGGAACCTGGGCCCATTCTAGAGGAGGAAGAGGCCTCCAGGCTGGGAAACAGGTCACTGGAGGGTCTT
  AGATCCCCAAAGCCCCTAGAGCTGAGTTCTCAGAACTTGAAGAGCTGGGGAGGAGGCCTTCTTGGCTCTT
  GGCTGCCCTCTGGCCTCAAGACCCTCAAACCCAGGTGCCTCCAACCTCAGAACATTTGGAACCCCACCTA
  TGGCTCCTGGACCCCAAGGGGCCCCCAGGGTGAAGATACAGGCATTGAACACTGCAATGGCTCCTCCTCA
  AGTTCCATTCCAGGCATCCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222631 representing NM_206941
  Red=Cloning site Green=Tags(s)
  
  LETRAAAPGSGGRGGAAGGGSGWTSRAHSPQAGRSPREGAEGGEGCAEAWAALRWAAAGGFGGGGGACAA
  GGGGGGYRGGDTSESDLLWADGEDGTSFVHPSGELYLQPLAVTEGHGEVEIRKHPNCSHCPFKDCQWQAE
  LWTAECTCPEGTELAVDNVTCMDLPTTASPLILMGAVVAALALSLLMMCAVLILVNQKCQGLWGTRLPGP
  ELELSKLRSSAIRTAPNPYYCQVGLSPAQPWPLPPGLTEVSPANVTLLRALGHGAFGEVYEGLVTGLPGD
  SSPLPVAIKTLPELCSHQDELDFLMEALIISKFSHQNIVRCVGLSFRSAPRLILLELMSGGDMKSFLRHS
  RPHPGQLAPLTMQDLLQLAQDIAQGCHYLEENHFIHRDIAARNCLLSCSGASRVAKIGDFGMARDIYQAS
  YYRKGGRTLLPVKWMPPEALLEGLFTSKTDSWSFGVLLWEIFSLGYMPYPGHTNQEVLDFIATGNRMDPP
  RNCPGPVYRIMTQCWQHQPELRPDFGSILERIQYCTQDPDVLNSPLPVEPGPILEEEEASRLGNRSLEGL
  RSPKPLELSSQNLKSWGGGLLGSWLPSGLKTLKPRCLQPQNIWNPTYGSWTPRGPQGEDTGIEHCNGSSS
  SSIPGIQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_206941
• ORF Size: 1911 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_206941.1, NP_996824.1
• RefSeq Size: 2452 bp
• RefSeq ORF: 1914 bp
• Locus ID: 17005
• UniProt ID: P08923
• Cytogenetics: 2 59.97 cM
• Gene Summary: The protein encoded by this gene is a member of the ros/insulin receptor family of tyrosine kinases. Tyrosine-specific phosphorylation of proteins is a key to the control of diverse pathways leading to cell growth and differentiation. Four alternatively spliced transcript variants encoding different isoforms have been described for this gene. These transcripts are expressed in a tissue-specific manner in lymphocytes, brain and neuroblastoma cells, and the encoded isoforms exhibit different subcellular localization. The lymphocyte and brain specific variants initiate translation at non-AUG (CUG) start codons. [provided by RefSeq, Jul 2008]