Ltk (NM_206941) Mouse Untagged Clone

CAT#: MC219975

Ltk (untagged) - Mouse leukocyte tyrosine kinase (Ltk), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ltk
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219975 representing NM_206941
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  CTGGAGACCCGCGCGGCGGCGCCGGGCAGCGGCGGGAGAGGAGGCGCGGCAGGTGGAGGGAGCGGCTGGA
  CGTCGCGAGCCCACTCTCCGCAGGCCGGACGCTCGCCGCGGGAAGGGGCCGAGGGCGGCGAGGGCTGCGC
  GGAGGCCTGGGCTGCGCTCCGCTGGGCTGCGGCGGGAGGCTTCGGGGGCGGCGGCGGGGCCTGCGCGGCG
  GGCGGCGGTGGCGGCGGCTACCGGGGCGGTGATACTTCTGAGTCTGACCTCCTCTGGGCTGATGGGGAAG
  ATGGCACATCCTTTGTCCACCCCAGTGGTGAGCTCTACCTACAGCCTCTGGCAGTCACAGAGGGCCATGG
  GGAGGTGGAGATCCGAAAGCATCCCAACTGCAGTCACTGCCCTTTCAAAGACTGCCAGTGGCAGGCAGAG
  CTCTGGACGGCCGAATGCACGTGCCCAGAGGGCACGGAGCTAGCTGTGGATAATGTCACTTGCATGGACC
  TGCCAACCACCGCAAGCCCTCTGATCCTGATGGGAGCTGTAGTGGCAGCCTTGGCACTGAGTCTCCTAAT
  GATGTGTGCAGTCCTGATTCTAGTGAACCAGAAGTGTCAGGGCCTGTGGGGGACCAGGCTGCCAGGCCCT
  GAGCTTGAGCTAAGCAAGCTTCGATCCTCTGCCATCAGGACAGCACCCAACCCTTACTATTGTCAGGTGG
  GACTCAGTCCTGCCCAGCCCTGGCCTTTGCCCCCAGGGCTCACTGAGGTTTCACCAGCCAATGTCACTCT
  ACTCAGAGCCCTTGGCCATGGTGCCTTTGGGGAAGTGTACGAGGGACTAGTGACTGGTCTTCCTGGGGAC
  TCCAGTCCTCTTCCAGTGGCTATTAAGACTCTGCCAGAGCTCTGCTCCCATCAGGATGAGCTGGATTTTC
  TCATGGAGGCTCTGATCATCAGCAAGTTCAGCCATCAGAACATTGTACGCTGTGTGGGGCTCAGCTTTCG
  GTCTGCCCCGCGCCTCATTCTGCTGGAGCTGATGTCTGGTGGGGACATGAAGAGCTTTTTGAGGCACAGC
  AGACCACACCCAGGACAACTGGCACCTCTGACCATGCAGGACCTATTGCAGCTGGCCCAGGATATAGCCC
  AGGGCTGCCACTACCTGGAGGAAAATCACTTCATTCACAGAGACATTGCTGCCCGTAACTGTCTGCTTAG
  CTGCAGTGGAGCCAGCCGAGTGGCCAAGATTGGAGATTTTGGAATGGCAAGAGATATCTACCAGGCCAGT
  TATTATCGCAAGGGTGGCCGGACCTTGCTCCCAGTCAAGTGGATGCCGCCAGAAGCTCTCCTGGAGGGCC
  TTTTCACATCCAAGACAGACTCCTGGTCTTTTGGGGTCCTGCTCTGGGAGATCTTCTCACTGGGGTATAT
  GCCCTACCCTGGACATACCAACCAGGAGGTTCTAGACTTCATTGCCACAGGGAACAGGATGGACCCTCCT
  AGGAACTGTCCTGGGCCAGTGTACCGAATCATGACCCAGTGTTGGCAGCATCAGCCGGAGCTCCGCCCTG
  ACTTTGGCAGCATCTTGGAACGGATTCAGTACTGCACTCAGGACCCTGATGTGCTGAACTCACCCCTGCC
  CGTGGAACCTGGGCCCATTCTAGAGGAGGAAGAGGCCTCCAGGCTGGGAAACAGGTCACTGGAGGGTCTT
  AGATCCCCAAAGCCCCTAGAGCTGAGTTCTCAGAACTTGAAGAGCTGGGGAGGAGGCCTTCTTGGCTCTT
  GGCTGCCCTCTGGCCTCAAGACCCTCAAACCCAGGTGCCTCCAACCTCAGAACATTTGGAACCCCACCTA
  TGGCTCCTGGACCCCAAGGGGCCCCCAGGGTGAAGATACAGGCATTGAACACTGCAATGGCTCCTCCTCA
  AGTTCCATTCCAGGCATCCAGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_206941
• Insert Size: 1914 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_206941.1, NP_996824.1
• RefSeq Size: 2452 bp
• RefSeq ORF: 1914 bp
• Locus ID: 17005
• UniProt ID: P08923
• Cytogenetics: 2 59.97 cM
• Gene Summary: The protein encoded by this gene is a member of the ros/insulin receptor family of tyrosine kinases. Tyrosine-specific phosphorylation of proteins is a key to the control of diverse pathways leading to cell growth and differentiation. Four alternatively spliced transcript variants encoding different isoforms have been described for this gene. These transcripts are expressed in a tissue-specific manner in lymphocytes, brain and neuroblastoma cells, and the encoded isoforms exhibit different subcellular localization. The lymphocyte and brain specific variants initiate translation at non-AUG (CUG) start codons. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) is missing several exons from the 5' end, and has a different 5' end exon compared to transcript variant 4. This results in a shorter isoform (B) with a different N-terminus compared to isoform D. This variant is specifically expressed in the brain and initiates translation from a non-AUG (CUG) start codon.