Fbxl5 (NM_178729) Mouse Tagged ORF Clone

CAT#: MG222589

Fbxl5 (tGFP-tagged) - Mouse F-box and leucine-rich repeat protein 5 (Fbxl5) transcript variant 2, (10ug)

Product Images

Circular map for MG222589

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fbxl5
• Synonyms: Fbl4; Fir4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222589 representing NM_178729
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCCTTCCCCGATGAAGTGGACGTCTTCACCGCCCCGCACTGGCGGATGAAGCAACTGGTGGGGC
  GCTACTGCGACAAGCTTTCTAAAACCAACTTTTCCAACAACAATGACTTCCGTGCTCTTCTCCAGTCTTT
  ATATGCTACTTTCAAGGAGTTTAAAATGCATGAACAAATTGAAAATGAATACATTATCGGTTTACTTCAG
  CAGCGCAGTCAGACCATATATAATGTACACTCTGACAATAAGCTCTCCGAGATGCTGAGTCTCTTTGAAA
  AGGGGCTGAAGAATGTCAAGAATGAGTACGAACAGTTAAATTATGCAAAACAGCTGAAAGAGAGATTGGA
  GGCTTTTACAAGAGATTTTCTTCCTCACATGAAAGAGGAAGAAGAGGTTTTTCAGCCCATGTTAATGGAA
  TATTTTACCTACGAGGAGCTTAAAGATATCAAAAAGAAAGTGATTGCACAGCACTGCTCACAGAAGGACA
  CGGCAGAGCTCCTCAGAGGGCTCAGTCTGTGGAACCAAGCTGAGGAGCGACAGAAAGTTCTCAAGTACTC
  TGTGGATGAGAAGGCAGATACAGAAGCGGAAGTGTCAGAACACTCCACAGGTATAACCCATCTTCCTCCT
  GAGGTAATGCTGTCCATTTTCAGTTACCTTAATCCTCAAGAATTGTGTCGGTGTAGTCAAGTCAGTACTA
  AGTGGTCTCAGCTGGCAAAAACAGGATCTTTGTGGAAACATCTTTACCCTGTTCATTGGGCAAGAGGTGA
  CTGGTATAGTGGTCCTGCCACTGAACTTGATACTGAGCCTGATGAGGAATGGGTGAGAAATAGGAAAGAT
  GAAAGTCGTGCTTTCCAGGAGTGGGATGAAGATGCCGATATAGATGAATCTGAAGAGTCTGCAGAGGAAT
  CGGTTGCTATCAGCATTGCACAAATGGAAAAACGTGTACTCCATGGCTTAATTCATAATGTTCTGCCATA
  TGTTGGTACCTCTGTAAAAACTTTAGTGTTAGCGTACAGCTCTGCAGTCTCCAGCAAAATGGTTAGGCAG
  ATTTTAGAGCTTTGTCCTAACCTGGAACATCTGGATCTTACCCAGACCGACATTTCTGATTCTGCATTTG
  ACAGTTGGTCTTGGCTCGGTTGCTGCCAGAGTCTTCGGCATCTTGATCTGTCTGGATGTGAAAAAATCAC
  AGACATGGCTCTAGAGAAGATTTCTAGAGCTCTTGGAGTTCTGACATCTCATCAGAGTGGCTTTCTGAAA
  AGTGCAGGCAAGGCTGCTTCAACTCCATGGACAAGTAAAGACATTACCATGCCATCCACCACGCAGTATG
  CCTGTTTGCACAATTTAACTGACAAAGGCATTGGTGAAGAAATAGACAACGAGCACTCTTGGACTGAACC
  TGTGTCTTCTGAAAGTTTGACTTCTCCTTATGTGTGGATGCTAGATGCTGAAGATTTGGCTGATATTGAA
  GATGCTGTAGAGTGGAGACACAGAAATGTTGAGAGTCTCTGTGTGATGGAAACAGCCTCCAACTTTGGTT
  GTTCCTCATCTGGTTGTTACAGTAAGGACATTGTGGGATTAAGGACTAGTGTCTGTTGGCAGCAGCATTG
  TGCTTCTCCAGCCTTTGCATATTGTGGTCATTCATTCTGTTGTACAGGGACAGCTTTAAGAACTATGACT
  ACACTCCCAGCGACTTCTGCAATGTGTAGAAAAGCATTAAGGACTACATTGCCCAGGGGGAAAGACTTAA
  TTTATTTTGGGAGTGAAAAGTCTGACCAAGAGACTGGACGAGTACTTTTGTTCCTCAGTCTGTCTGGATG
  TTACCAGATCACAGACCATGGTCTCAGGTGGGTGATCAGCCCTAGTTGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222589 representing NM_178729
  Red=Cloning site Green=Tags(s)
  
  MAPFPDEVDVFTAPHWRMKQLVGRYCDKLSKTNFSNNNDFRALLQSLYATFKEFKMHEQIENEYIIGLLQ
  QRSQTIYNVHSDNKLSEMLSLFEKGLKNVKNEYEQLNYAKQLKERLEAFTRDFLPHMKEEEEVFQPMLME
  YFTYEELKDIKKKVIAQHCSQKDTAELLRGLSLWNQAEERQKVLKYSVDEKADTEAEVSEHSTGITHLPP
  EVMLSIFSYLNPQELCRCSQVSTKWSQLAKTGSLWKHLYPVHWARGDWYSGPATELDTEPDEEWVRNRKD
  ESRAFQEWDEDADIDESEESAEESVAISIAQMEKRVLHGLIHNVLPYVGTSVKTLVLAYSSAVSSKMVRQ
  ILELCPNLEHLDLTQTDISDSAFDSWSWLGCCQSLRHLDLSGCEKITDMALEKISRALGVLTSHQSGFLK
  SAGKAASTPWTSKDITMPSTTQYACLHNLTDKGIGEEIDNEHSWTEPVSSESLTSPYVWMLDAEDLADIE
  DAVEWRHRNVESLCVMETASNFGCSSSGCYSKDIVGLRTSVCWQQHCASPAFAYCGHSFCCTGTALRTMT
  TLPATSAMCRKALRTTLPRGKDLIYFGSEKSDQETGRVLLFLSLSGCYQITDHGLRWVISPSC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178729
• ORF Size: 1869 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178729.4, NP_848844.1
• RefSeq Size: 2421 bp
• RefSeq ORF: 1872 bp
• Locus ID: 242960
• UniProt ID: Q8C2S5
• Cytogenetics: 5 B3
• Gene Summary: Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2. Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent degradation of DCTN1/p150-glued (By similarity).[UniProtKB/Swiss-Prot Function]