Rpgr (NM_001177951) Mouse Tagged ORF Clone

CAT#: MG222537

Rpgr (tGFP-tagged) - Mouse retinitis pigmentosa GTPase regulator (Rpgr) transcript variant 2, (10ug)

Product Images

Circular map for MG222537

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rpgr
• Synonyms: Rd9; Rp3h
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222537 representing NM_001177951
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAAGAGGGTCGCGATGGGGGTCCCAGGGGGTAGGGCAGCACCTTAGGCTCAATCGAGTCGCCCCTG
  CTATCTTTCCGAAGCAGGCACAGATTCCGTTCGCAGGCTTCGGCATGGCGGAATCTGAGTCACTGGTGCC
  CGATACAGGTGCTGTGTTTACGTTTGGAAAAACTAAATTTGCCGAAAATATTCCTAGCAAATTCTGGTTT
  AAAAATGACATACCCATATGTCTTTCATGTGGAGATGAACATACTGCTATTGTTACAGGAAATAATAAAT
  TGTACATGTTCGGCAGTAACAACTGGGGTCAGTTAGGATTAGGATCAAAAGCTGCTATCATCAAGCCAAC
  ATGTATCAAAGCTCTTAAGCCTGAGAAGGTGAAACTTGCTGCCTGTGGAAGGAACCACACCTTAGTTTCA
  ACAGATACTGGTGGCGTATATGCAGCTGGTGGAAATAATGAAGGTCAACTGGGGCTTGGTGACACTGACG
  ATAGAGACACCTTTCATCAAATTGTCTTCTTTACACCTGCTGATACCATTAAACAGCTCTCTGCTGGCGC
  CAATACATCCGCTGCTCTTACTGAGGATGGAAAACTTTTTATGTGGGGTGACAATTCTGAAGGGCAGATT
  GGTCTAGAAGATAAAAGTAATGTATGTATCCCTCATGAAGTGACTGTTGGAAAGCCAATTTCCTGGATCT
  CTTGTGGATATTACCATTCAGCTTTTGTAACAATGGATGGGGAGCTCTACACATTTGGAGAACCCGAGAA
  TGGGAAGTTGGGCCTTCCCAATGAGCTGCTGATGAATCACAGATCACCCCAGCGTGTGCTGGGCATTCCT
  GAGAGGGTCATTCAAGTGGCCTGTGGTGGAGGGCACACTGTGGTTCTCACAGAGAAAGTTGTGTATGCCT
  TTGGGCTGGGGCAGTTTGGACAACTGGGCCTTGGCACTTTTCTCTTTGAAACATCAGAACCCAAAATTAT
  TGAGCGTATTAAGGATCAGAAAATATGTCATATTTCCTGTGGAGAAAACCATACAGCTTTGATGACAGAA
  CTAGGCCTCCTGTATACTTTTGGAGACGGCCGACATGGAAAGTTAGGACTTGGGATGGAGAATTTCACCA
  ATCAGTTCTTTCCTACCTTGTGCTCTAACTTTTTGAGATTTGCAGTTCAATTGATTGCCTGTGGTGGATG
  TCATATGCTAGTTTTTGCCACTCCACGACTTGGTACAATAGATGAACCTAAATTTGAAGACGTATATGAG
  CCTTATATAAGTACAGGTTCTTTTTCCATCAATGACCTCTCCCCAAGAAGTTCACTGAATAGATCTTTAT
  CAGCACGTCTGCGGCGAAGAGAGCGGGAGAGACCCCCATGCTCAGCTTCAATGGTGGGAACACTGCCTCC
  ATTAGAGGGGACTTCTGCCTCCACTTCAGCTTATTTTTACCCCAGTTCACCCCCCTTCCATTTGTCTGTG
  AATAACTACCCAGAGAAAAGCCCCTCTGAATCAATGGAGCCACTGGACTCAGATTATTTTGAAGATAAAA
  TGAACAAAGACACAGAGACAGAAAATTCTTCAGCAGTGGATTCAGAAAACTTTGGTGAAACTAATGATAT
  CTTAAATATGACACATATGATGACTACGAGTTCCAATGAGAAGTTATTAGATTTTTCACCAATTCAAAAA
  CAACAGAATCAAGACACATTTGAGAAAGTGATGGAAAGTACACCGTGCACTGAAAATGAGGATAGTTATG
  AATATGAAGAAATGTCAAAAATAAAAGAAGTGACAGTGTACAAACAATATTTAGCAAAAGGGATCTACAT
  GATACGACCAGCCGAGATTCTGGAAGCATTTTCAGATGAGGAAGTGGGTAATGGCCTAGACCAGGTGGAG
  GAGCCGCGGGTCTTCACTGATGGAAAGGGCTTACAAAGCAAGCAAGTAGGAAAGGAAAGTGATGAAGAAA
  TAGTCTCTGAGAAGAAAACTGAGGTGATGGAAGTGGCAGATGTGAAAAAGATTAGAGAAAGTGAAGAGAA
  CTCAAAATCAGATTCACTTTTTGATGACTTACCAGATAAAACCATGAATTCTGAGAGTGAAGACAATAAA
  GATATTGCTGAGGAAAGGAGAAGCAGTGAGCAGAATATGACCTTTGACAGTGAAACAGAATTGGTAGAAG
  AACCAGACAGTTACATGGAATGTGAGAGGCACAGTGAGCAAGACAGTGCTGAGGAGTTGGAGCAGCCCAA
  GCTAGTAGAATATAGTAGCGAAGAGAAAGATGAGAAAGATGAGAAAGATGACGATGAAGTGGAGACTGAG
  AACTTATGGTACGACAGGAACTGTACTGAGCAAGAAACTGAGAATGTATTCAGAGCAACCAGATTTTTCC
  CAAAGTTCGATCTTAAGCATGATCATTTGTCAGGGATACCAGAGGAGCAGGAAGGACCAGAGGATTCAGA
  AGGAAATGTAGTAGTGGAGCAAGTAGTACAGGCACAAAAGGAAAATTTGGAATTTGAAGGAGACAGAAAG
  GAGGCAAAAGCAGAAGCCCCATCAGATGTCATTACAGAAAAAGAGGCTCCTCAACTTTCAGAAACTGTGA
  AACCAGAAGAAGGGGAAATGGATGAGGAAATTAGTATCCTGAATGTAGAAGACACTGTAGAAGAAGAAAG
  GAAGGAAGGAGAGAAGGAGATTGTAGAAGAAGGAAGTATACCTGAAACAGAAGGCTCAGAAACTATTGAT
  ATTACTGATGAGAAGCTAGATGAAGTCCTTAAAGAGGAAGACAGTGCCAGCCTTCTGCAACGGGCCCTTC
  GTGAGTACAATGAAAATCCAAAAGGACACATGTATGATCGTGTAAAGAGCAGCTCTTCAGAAATTCTGGG
  AGGTAATGATCCAACAAGTAAAGACATAAAAAAAGCTAAAAAATCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222537 representing NM_001177951
  Red=Cloning site Green=Tags(s)
  
  MPRGSRWGSQGVGQHLRLNRVAPAIFPKQAQIPFAGFGMAESESLVPDTGAVFTFGKTKFAENIPSKFWF
  KNDIPICLSCGDEHTAIVTGNNKLYMFGSNNWGQLGLGSKAAIIKPTCIKALKPEKVKLAACGRNHTLVS
  TDTGGVYAAGGNNEGQLGLGDTDDRDTFHQIVFFTPADTIKQLSAGANTSAALTEDGKLFMWGDNSEGQI
  GLEDKSNVCIPHEVTVGKPISWISCGYYHSAFVTMDGELYTFGEPENGKLGLPNELLMNHRSPQRVLGIP
  ERVIQVACGGGHTVVLTEKVVYAFGLGQFGQLGLGTFLFETSEPKIIERIKDQKICHISCGENHTALMTE
  LGLLYTFGDGRHGKLGLGMENFTNQFFPTLCSNFLRFAVQLIACGGCHMLVFATPRLGTIDEPKFEDVYE
  PYISTGSFSINDLSPRSSLNRSLSARLRRRERERPPCSASMVGTLPPLEGTSASTSAYFYPSSPPFHLSV
  NNYPEKSPSESMEPLDSDYFEDKMNKDTETENSSAVDSENFGETNDILNMTHMMTTSSNEKLLDFSPIQK
  QQNQDTFEKVMESTPCTENEDSYEYEEMSKIKEVTVYKQYLAKGIYMIRPAEILEAFSDEEVGNGLDQVE
  EPRVFTDGKGLQSKQVGKESDEEIVSEKKTEVMEVADVKKIRESEENSKSDSLFDDLPDKTMNSESEDNK
  DIAEERRSSEQNMTFDSETELVEEPDSYMECERHSEQDSAEELEQPKLVEYSSEEKDEKDEKDDDEVETE
  NLWYDRNCTEQETENVFRATRFFPKFDLKHDHLSGIPEEQEGPEDSEGNVVVEQVVQAQKENLEFEGDRK
  EAKAEAPSDVITEKEAPQLSETVKPEEGEMDEEISILNVEDTVEEERKEGEKEIVEEGSIPETEGSETID
  ITDEKLDEVLKEEDSASLLQRALREYNENPKGHMYDRVKSSSSEILGGNDPTSKDIKKAKKS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001177951
• ORF Size: 2916 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177951.1, NP_001171422.1
• RefSeq Size: 3187 bp
• RefSeq ORF: 2919 bp
• Locus ID: 19893
• Cytogenetics: X A1.1
• Gene Summary: Could be a guanine-nucleotide releasing factor (By similarity). Plays a role in ciliogenesis (By similarity). Probably regulates cilia formation by regulating actin stress filaments and cell contractility (By similarity). May be involved in microtubule organization and regulation of transport in primary cilia (By similarity). Plays an important role in photoreceptor integrity. Isoform 5 may play a critical role in spermatogenesis and in intraflagellar transport processes.[UniProtKB/Swiss-Prot Function]