Dcun1d4 (NM_178896) Mouse Tagged ORF Clone
CAT#: MG222373
- TrueORF®
Dcun1d4 (tGFP-tagged) - Mouse DCN1 defective in cullin neddylation 1 domain containing 4 (S. cerevisiae) (Dcun1d4) transcript variant B, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_178896" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Dcun1d4 |
Synonyms | AI836376 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG222373 representing NM_178896
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTCGGATGCTGCAGCTGTCAACTTTCAGCTGAACTCTCATCTCTCAACACTGGCAAGCATTCATA AGATCTACCATACCCTTAACAAGCTGAATCTGACAGAAGACGTTGGCCAAGACGATCACCAAACAGGAAG CCTGCGCTCTTGCAGTTCTTCGGACTGCTTTAGCAAAGTGATGCCACCCAGGAAAAAGAGAAGACCTGCC TCTGGAGACGACTTATCTGCAAAAAAAAGCAGGCATGACAGCATGTATAGGAAATACGAGTCAACTAGAA TAAAGACTGAAGAAGAGGCTTTCTCCAGTAAGCGGTGCCTGGAGTGGTTTTATGAATATGCAGGAACTGA GGATGCCGTAGGTCCCGAAGGCATGGAGAAGTTCTGTGAAGACATTGGTGTTGAACCAGAAAACGTAGTT ATGCTTGTCCTAGCTTGGAAATTGGATGCACAAAACATGGGCTATTTTACTCTACAGGAGTGGCTGAAAG GAATGACTTCCCTACAATGTGATACAACAGAAAAACTCAGAACTACTTTGGATTACTTAAGATCATTATT AAACGATACAACAAACTTTAAGCTCATTTACAGATACGCGTTTGACTTTGCACGGGAAAAGGACCAGCGC AGCCTAGACATAAACACTGCCAAGTGCATGTTGGGACTGTTATTAGGAAAAATCTGGCCCCTTTTTCCAG TTTTTCACCAATTCTTAGAGCAATCAAAATACAAAGTGATAAACAAAGACCAGTGGTGCAATGTTCTGGA GTTCAGCAGAACGATTAGTCTCGACCTCAGCAACTACGATGAAGACGGAGCATGGCCAGTTTTGTTGGAC GAATTTGTGGAATGGTATAAAGACAAGCAGATGTCC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG222373 representing NM_178896
Red=Cloning site Green=Tags(s) MHSDAAAVNFQLNSHLSTLASIHKIYHTLNKLNLTEDVGQDDHQTGSLRSCSSSDCFSKVMPPRKKRRPA SGDDLSAKKSRHDSMYRKYESTRIKTEEEAFSSKRCLEWFYEYAGTEDAVGPEGMEKFCEDIGVEPENVV MLVLAWKLDAQNMGYFTLQEWLKGMTSLQCDTTEKLRTTLDYLRSLLNDTTNFKLIYRYAFDFAREKDQR SLDINTAKCMLGLLLGKIWPLFPVFHQFLEQSKYKVINKDQWCNVLEFSRTISLDLSNYDEDGAWPVLLD EFVEWYKDKQMS SGPTRTRRLE - GFP Tag - V |
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_178896 |
ORF Size | 876 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_178896.5, NP_849227.1 |
RefSeq Size | 4143 bp |
RefSeq ORF | 879 bp |
Locus ID | 100737 |
UniProt ID | Q8CCA0 |
Cytogenetics | 5 C3.3 |
Gene Summary | Contributes to the neddylation of all cullins by transfering NEDD8 from N-terminally acetylated NEDD8-conjugating E2s enzyme to different cullin C-terminal domain-RBX complexes which are necessary for the activation of cullin-RING E3 ubiquitin ligases (CRLs).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212061 | Dcun1d4 (untagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 4 (S. cerevisiae) (Dcun1d4), transcript variant B, (10ug) |
USD 330.00 |
|
MR222373 | Dcun1d4 (Myc-DDK-tagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 4 (S. cerevisiae) (Dcun1d4), transcript variant B |
USD 330.00 |
|
MR222373L3 | Lenti ORF clone of Dcun1d4 (Myc-DDK-tagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 4 (S. cerevisiae) (Dcun1d4), transcript variant B |
USD 630.00 |
|
MR222373L4 | Lenti ORF clone of Dcun1d4 (mGFP-tagged) - Mouse DCN1, defective in cullin neddylation 1, domain containing 4 (S. cerevisiae) (Dcun1d4), transcript variant B |
USD 630.00 |
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