Ksr2 (NM_001114545) Mouse Tagged ORF Clone

CAT#: MG222324

Ksr2 (tGFP-tagged) - Mouse kinase suppressor of ras 2 (Ksr2) transcript variant 1, (10ug)

Product Images

Circular map for MG222324

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ksr2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222324 representing NM_001114545
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAGGAAAACATGACGAAAAGCGAAGAGCAGCAACCTCTGAGTTTGCAAAAAGCCTTGCAGCAGT
  GCGAGTTGGTTCAGAACATGATCGACCTGAGCATCTCCAATCTGGAAGGGCTTAGGACCAAGTGTGCAGC
  TTCCAACGACCTCACGCAGAAGGAGATCAGGACCCTGGAGAGCAAGCTGGTGAAGTACTTCAGCCGACAG
  CTGTCCTGCAAGAAGAAGGTGGCTCTGCAGGAACGCAATGCTGAACTGGACGGCTTCCCCCAGCTCCGCC
  ACTGGTTCCGGATCGTGGATGTCCGAAAGGAAGTCCTGGAGGAGATCTCCCCTGACCAGCTGAGCTTGGA
  GGACCTCCTGGAGATGACCGACGAGCAGGTGTGTGAGACCGTGGAGAAGTATGGAGCCAACCAGGAAGAG
  TGCGCCCGGCTCAACGCCTCACTCTCCTGCCTCAGGAATGTGCACAAATCAGGAGGGAATCTCTCCAAAC
  AAGATTGGATCATCCAGTGGCCCACCACGGAGCCAGGGCAGGAGAGCAACCCTGTGTGCCCCCCGGAGCC
  CAGCCCGTGGATCCGAACCCACCTCTCCCAGAGCCCCAGGGTCCAGACCAAGTGTCCCCAGCACTTCTGT
  CCTACCAGCCCTACGCCTGGGACCCCTGTGTACACCCAAGTGGACAGACTCACTGTAGATGCCTACCCGA
  ACTTGTGCCCGCCGCCGCCACCCCTGGAATCAGGCCACCGCTCTCTGCCCCCGTCTCCCCGGCAGCGGCA
  CGTAGTCCGCACCCCACCTCGCACCCCAAATATTGTCACCACCGTGACCCCACCTGGCACGCCACCCATG
  AGGAGAAAAAACAAGCTGAAGCCTCCCGGGACGCCACCCCCCTCCTCTCGGAAGCTCATCCATCTGATCC
  CTGGCTTCACAGCGCTGCACAGGAGCAAATCCCACGAGTTCCAGCTCGGGAACCGTGTAGACGAGGCCAA
  CACACCCAAAGCCAAGAAGAAGAGCAAACCCTTGAACCTCAAGATCCACAGCGGCGTGGGCAGCTGCGAG
  AACATCCCCGCCCAGCAACGCTCTCCGCTCCTGTCCGAGCGCTCCTTGCGGTCCTTCTTTGTGGGCCACG
  GGCCCTTCCTGCCCTCCACCCCACCTGTCCACACTGAAGCCAATTTCTCTGCAAACACACTCTCTGTACC
  TCGCTGGTCACCACAGATCCCACGCAGAGACCTTGGCAATTCCATCAAGCACAGGTTTTCCACCAAGTAC
  TGGATGTCTCAGACGTGCACGGTCTGCGGGAAAGGGATGCTTTTTGGCCTCAAGTGTAAAAACTGCAAGT
  TAAAGTGCCACAATAAATGCACCAAAGAAGCCCCGCCCTGTCACCTCCTGATTATCCACCGAGGAGACTC
  TCTCTGTTGTTTTTACCCTACGGACCCAGCAAGGTTAGTGCGAACGGAGTCGGTGCCCTGTGACATCAAC
  AACCCGGTCCGGAAGCCTGCCCGATATTCAGATCTCCACATCAGTCAGACTCTCCCCAAAACCAACAAAA
  TCAACAAGGACCACATCCCTGTCCCTTACCAGCCAGACTCCAGCAGCAATCCCTCCTCCACGACGTCCTC
  CACGCCCTCCTCACCGGCACCCCCCCTCCCTCCCAGCGCCACGCCGCCTTCTCCCCTGCACCCCTCCCCG
  CAGTGCCCGAGACAGAAAAAGAACTTCAACCTGCCAGCTTCCCACTACTACAAATACAAGCAACAGTTCA
  TCTTCCCAGATGTGGTGCCTGTACCAGAGACACCCACCCGGGCACCCCAAGTCATCCTGCATCCTGTCAC
  CTCAAATACAATCTTGGAAGGAAACCCATTACTTCAAATTGAAGTGGAACCAACCTCCGAGAACGAAGAG
  AGCCACAATGAAGCAGAGGAGTCCGAGGACGAGTTTGAAGAGATGAACCTATCCCTCCTCTCAGCCCGGA
  GCTTCCCACGCAAAGCCAGCCAGACCAGCATCTTCCTTCAGGAGTGGGACATCCCGTTTGAACAGCTAGA
  GATTGGCGAGCTCATCGGGAAGGGTCGCTTCGGGCAGGTTTACCACGGACGCTGGCACGGCGAGGTGGCC
  ATCCGGTTGATTGACATAGAGAGGGACAATGAGGACCAGCTTAAGGCCTTCAAGCGGGAAGTGATGGCCT
  ACAGGCAGACGAGGCACGAGAACGTGGTGCTCTTCATGGGAGCCTGCATGAGCCCACCCCACTTGGCCAT
  CATCACCAGCCTCTGTAAGGGACGGACACTCTACTCTGTGGTGAGGGACGCCAAGATTGTCTTGGATGTC
  AACAAAACCAGGCAGATCGCGCAAGAGATTGTAAAGGGCATGGGCTACCTCCACGCCAAGGGGATCCTTC
  ATAAGGACCTCAAATCGAAGAACGTCTTCTATGACAACGGCAAAGTGGTCATCACAGACTTTGGGCTCTT
  CAGCATTTCCGGGGTGCTGCAGGCTGGCCGGCGGGACGACAAACTGCGGATCCAGAACGGCTGGCTGTGT
  CACCTGGCCCCAGAAATCATCCGCCAGCTGTCCCCTGACACAGAAGAGGACAAGCTCCCATTCTCCAAGC
  ACTCGGATGTCTTTGCGCTTGGCACCATCTGGTATGAGCTCCACGCCAGGGAGTGGCCATTCAAGACCCA
  ACCAGCAGAGGCAATCATTTGGCAGATGGGCACGGGAATGAAGCCCAACCTCAGCCAGATTGGCATGGGA
  AAAGAAATCTCGGACATACTGCTCTTCTGCTGGGCCTTCGAGCAGGAAGAGCGGCCCACCTTTACCAAGC
  TCATGGACATGCTAGAGAAACTGCCAAAGCGAAACCGCCGCCTGTCGCACCCCGGACATTTCTGGAAGTC
  TGCAGAGCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222324 representing NM_001114545
  Red=Cloning site Green=Tags(s)
  
  MDEENMTKSEEQQPLSLQKALQQCELVQNMIDLSISNLEGLRTKCAASNDLTQKEIRTLESKLVKYFSRQ
  LSCKKKVALQERNAELDGFPQLRHWFRIVDVRKEVLEEISPDQLSLEDLLEMTDEQVCETVEKYGANQEE
  CARLNASLSCLRNVHKSGGNLSKQDWIIQWPTTEPGQESNPVCPPEPSPWIRTHLSQSPRVQTKCPQHFC
  PTSPTPGTPVYTQVDRLTVDAYPNLCPPPPPLESGHRSLPPSPRQRHVVRTPPRTPNIVTTVTPPGTPPM
  RRKNKLKPPGTPPPSSRKLIHLIPGFTALHRSKSHEFQLGNRVDEANTPKAKKKSKPLNLKIHSGVGSCE
  NIPAQQRSPLLSERSLRSFFVGHGPFLPSTPPVHTEANFSANTLSVPRWSPQIPRRDLGNSIKHRFSTKY
  WMSQTCTVCGKGMLFGLKCKNCKLKCHNKCTKEAPPCHLLIIHRGDSLCCFYPTDPARLVRTESVPCDIN
  NPVRKPARYSDLHISQTLPKTNKINKDHIPVPYQPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSP
  QCPRQKKNFNLPASHYYKYKQQFIFPDVVPVPETPTRAPQVILHPVTSNTILEGNPLLQIEVEPTSENEE
  SHNEAEESEDEFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVA
  IRLIDIERDNEDQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDV
  NKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRRDDKLRIQNGWLC
  HLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMG
  KEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAEL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001114545
• ORF Size: 2880 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001114545.2, NP_001108017.2
• RefSeq Size: 2856 bp
• RefSeq ORF: 2883 bp
• Locus ID: 333050
• Cytogenetics: 5 F
• Gene Summary: Location-regulated scaffold connecting MEK to RAF. Has very low protein kinase activity and can phosphorylate MAP2K1 at several Ser and Thr residues with very low efficiency (in vitro). Interaction with BRAF enhances KSR2-mediated phosphorylation of MAP2K1 (in vitro). Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production (By similarity).[UniProtKB/Swiss-Prot Function]