Nr6a1 (NM_001159548) Mouse Tagged ORF Clone

CAT#: MG222287

Nr6a1 (tGFP-tagged) - Mouse nuclear receptor subfamily 6 group A member 1 (Nr6a1) transcript variant 2, (10ug)

Product Images

Circular map for MG222287

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nr6a1
• Synonyms: 1700113M01Rik; Gcnf; NCNF; RTR
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222287 representing NM_001159548
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACATGGGAAGTTTCCGTCCCAGATGATCGAGCTGAACAACGAACCTGTCTCATCTGTGGGGACC
  GCGCTACGGGCTTGCACTATGGGATCATCTCCTGCGAGGGCTGCAAGGGGTTTTTCAAGAGGAGCATTTG
  CAACAAACGGGTGTATCGGTGCAGTCGTGACAAGAACTGTGTCATGTCCCGGAAGCAGAGGAACAGATGT
  CAGTACTGCCGCCTGCTCAAGTGTCTCCAGATGGGCATGAACAGGAAGGCTATCAGAGAAGATGGCATGC
  CTGGAGGCCGGAACAAGAGCATTGGACCAGTCCAGATATCAGAAGAAGAAATTGAAAGAATCATGTCTGG
  ACAGGAGTTTGAGGAAGAAGCCAATCACTGGAGCAACCATGGTGACAGCGACCACAGTTCCCCTGGGAAC
  AGGGCTTCAGAGAGCAACCAGCCCTCACCAGGCTCCACACTATCATCCAGTAGGTCTGTGGAACTAAATG
  GATTCATGGCATTCAGGGATCAGTACATGGGGATGTCAGTGCCTCCACATTATCAATACATACCACACCT
  TTTTAGCTATTCTGGCCACTCACCACTTTTGCCCCCACAAGCTCGAAGCCTGGACCCTCAGTCCTACAGT
  CTGATTCATCAGCTGATGTCAGCCGAAGACCTGGAGCCATTGGGCACACCTATGTTGATTGAAGATGGGT
  ATGCTGTGACACAGGCAGAACTGTTTGCTCTGCTTTGCCGCCTGGCCGACGAGTTGCTCTTTAGGCAGAT
  TGCCTGGATCAAGAAGCTGCCTTTCTTCTGCGAGCTCTCAATCAAGGATTACACGTGCCTCTTGAGCTCT
  ACGTGGCAGGAGTTAATCCTGCTCTCCTCCCTCACAGTGTACAGCAAGCAGATCTTTGGGGAGCTGGCTG
  ATGTCACAGCCAAGTACTCACCCTCTGATGAAGAACTCCACAGATTTAGTGATGAAGGGATGGAGGTGAT
  TGAACGACTCATCTACCTATATCACAAGTTCCATCAGCTGAAGGTCAGCAACGAGGAGTACGCATGCATG
  AAAGCAATTAACTTCCTGAATCAAGATATCAGGGGTCTGACCAGTGCCTCACAGCTGGAACAACTGAACA
  AGCGGTATTGGTACATTTGTCAGGATTTCACTGAATATAAATACACACATCAGCCAAACCGCTTTCCTGA
  TCTTATGATGTGCTTGCCAGAGATCCGATACATCGCAGGCAAGATGGTGAATGTGCCCCTGGAGCAGCTG
  CCCCTCCTCTTTAAGGTGGTGCTGCACTCCTGCAAGACAAGTACGGTGAAGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222287 representing NM_001159548
  Red=Cloning site Green=Tags(s)
  
  METWEVSVPDDRAEQRTCLICGDRATGLHYGIISCEGCKGFFKRSICNKRVYRCSRDKNCVMSRKQRNRC
  QYCRLLKCLQMGMNRKAIREDGMPGGRNKSIGPVQISEEEIERIMSGQEFEEEANHWSNHGDSDHSSPGN
  RASESNQPSPGSTLSSSRSVELNGFMAFRDQYMGMSVPPHYQYIPHLFSYSGHSPLLPPQARSLDPQSYS
  LIHQLMSAEDLEPLGTPMLIEDGYAVTQAELFALLCRLADELLFRQIAWIKKLPFFCELSIKDYTCLLSS
  TWQELILLSSLTVYSKQIFGELADVTAKYSPSDEELHRFSDEGMEVIERLIYLYHKFHQLKVSNEEYACM
  KAINFLNQDIRGLTSASQLEQLNKRYWYICQDFTEYKYTHQPNRFPDLMMCLPEIRYIAGKMVNVPLEQL
  PLLFKVVLHSCKTSTVKE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159548
• ORF Size: 1314 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159548.1, NP_001153020.1
• RefSeq Size: 5877 bp
• RefSeq ORF: 1317 bp
• Locus ID: 14536
• UniProt ID: Q64249
• Cytogenetics: 2 B
• Gene Summary: Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis.[UniProtKB/Swiss-Prot Function]