Nr6a1 (NM_001159548) Mouse Untagged Clone

CAT#: MC215789

Nr6a1 (untagged) - Mouse nuclear receptor subfamily 6, group A, member 1 (Nr6a1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nr6a1
• Synonyms: 1700113M01Rik; Gcnf; NCNF; RTR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215789 representing NM_001159548
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACATGGGAAGTTTCCGTCCCAGATGATCGAGCTGAACAACGAACCTGTCTCATCTGTGGGGACC
  GCGCTACGGGCTTGCACTATGGGATCATCTCCTGCGAGGGCTGCAAGGGGTTTTTCAAGAGGAGCATTTG
  CAACAAACGGGTGTATCGGTGCAGTCGTGACAAGAACTGTGTCATGTCCCGGAAGCAGAGGAACAGATGT
  CAGTACTGCCGCCTGCTCAAGTGTCTCCAGATGGGCATGAACAGGAAGGCTATCAGAGAAGATGGCATGC
  CTGGAGGCCGGAACAAGAGCATTGGACCAGTCCAGATATCAGAAGAAGAAATTGAAAGAATCATGTCTGG
  ACAGGAGTTTGAGGAAGAAGCCAATCACTGGAGCAACCATGGTGACAGCGACCACAGTTCCCCTGGGAAC
  AGGGCTTCAGAGAGCAACCAGCCCTCACCAGGCTCCACACTATCATCCAGTAGGTCTGTGGAACTAAATG
  GATTCATGGCATTCAGGGATCAGTACATGGGGATGTCAGTGCCTCCACATTATCAATACATACCACACCT
  TTTTAGCTATTCTGGCCACTCACCACTTTTGCCCCCACAAGCTCGAAGCCTGGACCCTCAGTCCTACAGT
  CTGATTCATCAGCTGATGTCAGCCGAAGACCTGGAGCCATTGGGCACACCTATGTTGATTGAAGATGGGT
  ATGCTGTGACACAGGCAGAACTGTTTGCTCTGCTTTGCCGCCTGGCCGACGAGTTGCTCTTTAGGCAGAT
  TGCCTGGATCAAGAAGCTGCCTTTCTTCTGCGAGCTCTCAATCAAGGATTACACGTGCCTCTTGAGCTCT
  ACGTGGCAGGAGTTAATCCTGCTCTCCTCCCTCACAGTGTACAGCAAGCAGATCTTTGGGGAGCTGGCTG
  ATGTCACAGCCAAGTACTCACCCTCTGATGAAGAACTCCACAGATTTAGTGATGAAGGGATGGAGGTGAT
  TGAACGACTCATCTACCTATATCACAAGTTCCATCAGCTGAAGGTCAGCAACGAGGAGTACGCATGCATG
  AAAGCAATTAACTTCCTGAATCAAGATATCAGGGGTCTGACCAGTGCCTCACAGCTGGAACAACTGAACA
  AGCGGTATTGGTACATTTGTCAGGATTTCACTGAATATAAATACACACATCAGCCAAACCGCTTTCCTGA
  TCTTATGATGTGCTTGCCAGAGATCCGATACATCGCAGGCAAGATGGTGAATGTGCCCCTGGAGCAGCTG
  CCCCTCCTCTTTAAGGTGGTGCTGCACTCCTGCAAGACAAGTACGGTGAAGGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159548
• Insert Size: 1317 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159548.1, NP_001153020.1
• RefSeq Size: 5877 bp
• RefSeq ORF: 1317 bp
• Locus ID: 14536
• UniProt ID: Q64249
• Cytogenetics: 2 B
• Gene Summary: Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.