Sorbs1 (NM_001034963) Mouse Tagged ORF Clone

CAT#: MG222282

Sorbs1 (tGFP-tagged) - Mouse sorbin and SH3 domain containing 1 (Sorbs1) transcript variant 4, (10ug)

Product Images

Circular map for MG222282

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sorbs1
• Synonyms: 2310065E01Rik; 9530001P15Rik; CAP; mKIAA1296; Sh3d5; SH3P12
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222282 representing NM_001034963
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCTGAATGTGATGTTGGAAGCTCTAAAGCTGTGGTGAATGGCTTGGCATCTGGCAACCATGGAC
  CAGACAAAGACATGGACCCTACCAAAATCTGCACTGGGAAAGGAACAGTGACTCTTCGGGCCTCGTCTTC
  CTACAGGGGAACCCCAAGCAGCAGCCCTGTGAGCCCCCAGGAATCTCCGAAGCATGAAAGCAAGTCAGAT
  GAATGGAAACTTTCTTCCAGTGCAGATACCAATGGCAACGCCCAGCCCTCCCCACTTGCTGCCAAGGGCT
  ATAGAAGTGTGCATCCCAGCCTTTCTGCTGACAAGCCCCAGGGCAGTCCTTTACTAAACGAAGTTTCTTC
  TTCCCACATTGAAACCGATTCCCAAGACTTCCCTCCAACAAGCAGACCTTCGTCTGCCTACCCCTCCACC
  ACCATCGTCAACCCTACCATTGTGCTCCTGCAGCACAATCGAGATCCTGCCTCAGAGAGAAGAGCGGGTG
  AGCAGGACCCAGTACCAACCCCAGCAGAACTCACTTCGCCCGGCAGGGCTTCTGAGAGAAGGGCAAAGGA
  TGCTAGCAGACGGGTGGTGAGGAGCACACAGGACCTGAGCGATGTGTCTACAGATGAAGTGGGCATCCCA
  CTCCGGAATACCGAGCGATCGAAAGACTGGTACAAAACTATGTTTAAACAGATCCACAAACTGAACAGAG
  ATGATGATTCTGATGTCCATTCCCCTCGATATTCCTTCTCTGACGACACAAAGTCTCCCCTTTCTGTGCC
  TCGCTCAAAAAGTGAGATGAACTACATCGAAGGGGAGAAAGTGGTTAAGAGGTCCGCCACACTCCCGCTC
  CCAGCCCGCTCCTCCTCACTCAAGTCCAGCCCGGAAAGAAACGACTGGGAGCCCCCAGATAAGAAAGTGG
  ATACGAGAAAATACCGAGCAGAGCCCAAAAGCATTTACGAATATCAGCCGGGCAAGTCTTCGGTCCTGAC
  CAATGAGAAGATGAGTCGGGATATAAGCCCAGAAGAGATAGATTTAAAGAATGAACCTTGGTATAAATTC
  TTTTCGGAATTGGAGTTTGGGAGACCGCCTCCCAAAAAGATATGGGACTATACTCCTGGAGACTGCTCTA
  TCCTTCCTAGAGAGGATAGAAAGAGCTCAGCAGTCAGCCCGACTCCAGACATTACGTCAGAGCCTCCTGG
  ATATATCTATTCTTCCAACTTCCATGCAGTGAAGAGAGAATCGGACGGGACCCCCGGGGGTCTCGCTAGC
  TTGGAGAATGAGAGGCAGATCTATAAGAGTGTCTTGGAAGGTGGCGACATCCCTCTTCAGGGCCTCAGTG
  GGCTCAAGCGACCTTCCAGCTCAGCTTCCACTAAAGATTCAGAGTCACCAAGACATTTTATACCAGCTGA
  TTACTTGGAGTCCACAGAAGAATTTATTCGGAGACGGCACGATGATAAAGAGATGAGACCTGCTCGAGCC
  AAATTTGACTTTAAAGCCCAGACCCTGAAGGAGCTGCCTCTGCAGAAGGGAGACGTTGTTTACATCTACA
  GACAGATTGACCAGAACTGGTATGAAGGTGAACACCATGGCCGGGTGGGAATCTTCCCACGCACCTATAT
  CGAGCTTCTTCCTCCAGCTGAGAAGGCTCAGCCCAGAAAGTTGGCACCCGTACAAGTTTTGGAATATGGA
  GAAGCCATTGCGAAGTTTAACTTTAATGGAGATACACAAGTAGAAATGTCTTTCCGAAAGGGGGAGAGGA
  TCACGCTGCTCCGACAGGTGGATGAGAACTGGTACGAAGGGAGGATTCCTGGGACATCTCGCCAAGGCAT
  TTTCCCTATCACCTATGTAGATGTGCTTAAGAGGCCATTGGTGAAAACCCCTGTGGATTACATCGACCTG
  CCTTATTCTTCTTCCCCAAGTCGCAGTGCCACTGTGAGCCCACAGCAACCTCAAGCCCAGCAGCGAAGAG
  TCACCCCAGACAGGAGTCAGCCCTCACTGGATTTGTGTAGCTACCAAGCGTTATATAGTTATGTGCCACA
  GAACGATGATGAGTTGGAACTCCGAGATGGAGATATTGTTGATGTCATGGAAAAATGTGACGATGGATGG
  TTTGTTGGCACTTCGAGAAGGACGAGGCAGTTTGGTACTTTTCCAGGCAACTATGTAAAACCTTTATATC
  TA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222282 representing NM_001034963
  Red=Cloning site Green=Tags(s)
  
  MSSECDVGSSKAVVNGLASGNHGPDKDMDPTKICTGKGTVTLRASSSYRGTPSSSPVSPQESPKHESKSD
  EWKLSSSADTNGNAQPSPLAAKGYRSVHPSLSADKPQGSPLLNEVSSSHIETDSQDFPPTSRPSSAYPST
  TIVNPTIVLLQHNRDPASERRAGEQDPVPTPAELTSPGRASERRAKDASRRVVRSTQDLSDVSTDEVGIP
  LRNTERSKDWYKTMFKQIHKLNRDDDSDVHSPRYSFSDDTKSPLSVPRSKSEMNYIEGEKVVKRSATLPL
  PARSSSLKSSPERNDWEPPDKKVDTRKYRAEPKSIYEYQPGKSSVLTNEKMSRDISPEEIDLKNEPWYKF
  FSELEFGRPPPKKIWDYTPGDCSILPREDRKSSAVSPTPDITSEPPGYIYSSNFHAVKRESDGTPGGLAS
  LENERQIYKSVLEGGDIPLQGLSGLKRPSSSASTKDSESPRHFIPADYLESTEEFIRRRHDDKEMRPARA
  KFDFKAQTLKELPLQKGDVVYIYRQIDQNWYEGEHHGRVGIFPRTYIELLPPAEKAQPRKLAPVQVLEYG
  EAIAKFNFNGDTQVEMSFRKGERITLLRQVDENWYEGRIPGTSRQGIFPITYVDVLKRPLVKTPVDYIDL
  PYSSSPSRSATVSPQQPQAQQRRVTPDRSQPSLDLCSYQALYSYVPQNDDELELRDGDIVDVMEKCDDGW
  FVGTSRRTRQFGTFPGNYVKPLYL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001034963
• ORF Size: 2172 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034963.1, NP_001030135.1
• RefSeq Size: 5312 bp
• RefSeq ORF: 2175 bp
• Locus ID: 20411
• UniProt ID: Q62417
• Cytogenetics: 19 34.25 cM
• Gene Summary: Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions.[UniProtKB/Swiss-Prot Function]