Ccng1 (NM_009831) Mouse Tagged ORF Clone

CAT#: MG222139

Ccng1 (tGFP-tagged) - Mouse cyclin G1 (Ccng1), (10ug)

Product Images

Circular map for MG222139

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ccng1
• Synonyms: AI314029
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222139 representing NM_009831
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAGAAGTACTGACAACTGACTCTCAGAAACTGCTACACCAGCTGAACACCCTGTTGGAACAGGAGT
  CTAGATGTCAGCCAAAGGTCTGCGGCTTGAAACTAATTGAGTCGGCCCATGATAATGGCCTCAGAATGAC
  TGCAAGATTACGGGACTTTGAAGTAAAAGATCTACTTAGTCTAACTCAGTTCTTTGGCTTTGACACGGAG
  ACATTTTCCCTAGCTGTGAATTTACTGGACAGATTCTTGTCTAAAATGAAGGTACAGGCGAAGCATCTTG
  GGTGTGTTGGACTGAGCTGCTTTTATTTGGCTGTGAAAGCGACTGAAGAGGAAAGGAATGTCCCACTGGC
  GACTGATTTGATCCGAATAAGTCAGTATAGGTTCACGGTTTCAGACCTGATGAGAATGGAGAAGATTGTG
  TTGGAGAAAGTGTGTTGGAAAGTCAAAGCTACTACTGCCTTTCAATTTCTGCAGCTCTATTATTCACTCG
  TTCACGACACCTTGCCATTTGAGAGGAGAAACGATCTGAATTTTGAAAGACTAGAAGCCCAACTTAAGGC
  CTGCCACTGCAGGATCATATTTTCTAAGGCAAAGCCTTCTGTGCTGGCGCTATCTATCCTTGCGTTGGAG
  ATCCAAGCACTGAAATACGTAGAGTTAACAGAAGGAGTAGAATGTATTCAGAAACATTCCAAGATAAGTG
  GCCGAGATTTGACCTTCTGGCAAGAGCTTGTTTCCAAGTGTTTAACTGAATATTCATCAAACAAGTGCTC
  CAAACCTAACGGTCAGAAGTTGAAATGGATTGTGTCTGGACGCACTGCACGGCAACTGAAGCACAGTTAT
  TATAGAATAACTCACCTCCCAACGATTCCTGAGACCATTTGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222139 representing NM_009831
  Red=Cloning site Green=Tags(s)
  
  MIEVLTTDSQKLLHQLNTLLEQESRCQPKVCGLKLIESAHDNGLRMTARLRDFEVKDLLSLTQFFGFDTE
  TFSLAVNLLDRFLSKMKVQAKHLGCVGLSCFYLAVKATEEERNVPLATDLIRISQYRFTVSDLMRMEKIV
  LEKVCWKVKATTAFQFLQLYYSLVHDTLPFERRNDLNFERLEAQLKACHCRIIFSKAKPSVLALSILALE
  IQALKYVELTEGVECIQKHSKISGRDLTFWQELVSKCLTEYSSNKCSKPNGQKLKWIVSGRTARQLKHSY
  YRITHLPTIPETIC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009831
• ORF Size: 882 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009831.2, NP_033961.1
• RefSeq Size: 3501 bp
• RefSeq ORF: 885 bp
• Locus ID: 12450
• UniProt ID: P51945
• Cytogenetics: 11 A5
• Gene Summary: May play a role in growth regulation. Is associated with G2/M phase arrest in response to DNA damage. May be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation.[UniProtKB/Swiss-Prot Function]