Adgrg2 (NM_001079848) Mouse Tagged ORF Clone

CAT#: MG222104

Gpr64 (tGFP-tagged) - Mouse G protein-coupled receptor 64 (Gpr64) transcript variant 4, (10ug)

Product Images

Circular map for MG222104

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adgrg2
• Synonyms: AW212196; B830041D06Rik; Gpr64; Me6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222104 representing NM_001079848
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTTCTCTGGTGGGCAGTACAGCCCTGTTGGCAGACCTGAAGAGGTTTTACTGATATACAAGATAT
  TCCTTGTCATCATTTGTTTTCATGTCATTCTCGTTACATCCCTGAAAGATGCTGCTTCAGAAGTTTTGTC
  GACTTTAAACAAAACAGAAAAATCTAAAATCACTATAGTAAAAACCTTCAATGCATCAGGAGTCAAATCC
  CAGAGAAATATCTGCAATTTGTCATCTCTTTGCAATGACTCAGTATTTTTTAGAGGTGAGATAGTGTTTC
  AACATGATGAAGACCACAATGTTACCCAGAATCAAGATACAGCTAATGGCACCTTCGCTGGAGTCCTGTC
  TCTAAGTGAACTGAAGCGATCAGAGCTCAACAAAACTCTACAGACCTTAAGTGAGACTTACTTTATAGTG
  TGTGCTACAGCAGAGGCCCAAAGCACGGTAAACTGTACATTCACAGTAAAACTCAATGAGACCATGAATG
  TGTGTGCCATGATGGTTACTTTCCAAACTGTACAGATTCGGCCAATGGAACAGTGCTGCTGTTCCCCGAG
  GACTCCCTGCCCTTCCTCACCAGAAGAGTTAGAAAAACTACAGTGTGAACTGCAGGATCCCATTGTCTGT
  CTTGCTGATCAACCGCATGGCCCACCGTTATCGTCTTCCAGCAAGCCTGTTGTACCTCAGGCCACCATTA
  TTTCCCATGTTGCTAGTGACTTCTCTTTGGCTGAACCCCTTGATCATGCCCTTATGACCCCAAGCACACC
  CTCTCTGACACAAGAAAGTAACCTTCCATCTCCTCAGCCTACGATCCCCCTGGCTTCCAGTCCTGCCACT
  GACTTGCCAGTTCAATCTGTAGTGGTCTCTTCTTTGCCTCAAACTGATCTTTCCCACACCCTGTCACCGG
  TGCAGTCCTCCATTCCCTCTCCTACCACACCAGCCCCATCTGTCCCTACAGAACTGGTCACCATCAGCAC
  ACCTCCTGGTGAGACAGTTGTCAACACTAGCACTGTTTCTGATCTGGAAGCCCAAGTATCCCAGATGGAG
  AAAGCTTTGTCTTTGGGTAGCTTAGAGCCTAATCTTGCAGGCGAAATGGTAAACCGAGTCAGCAAACTCC
  TTCACTCTCCACCTGCCTTGCTAGCCCCTCTAGCTCAAAGGTTGCTAAAAGTGGTAGATGCCATTGGCTT
  ACAGCTGAATTTTTCATCTACAACTATCAGTCTAACTTCACCTTCTTTGGCTCTTGCTGTGATCAGAGTG
  AATGCCAGTAATTTCAATACCACGACTTTTGCAGCCCAAGACCCAACAAATCTCCAGGTTTCTCTGGAAA
  CCCCACCTCCTGAGAATAGTATTGGTGCCATTACTCTGCCCTCATCACTGATGAATAATTTGCCAGCTAA
  TGATGTAGAATTGGCTTCAAGGATTCAGTTCAATTTCTTTGAAACACCCGCCCTGTTTCAGGATCCTTCC
  CTGGAGAACCTCACTCTGATAAGCTATGTCATATCATCAAGTGTCACAAACATGACCATCAAGAACTTGA
  CAAGAAACGTGACAGTCGCACTGAAACACATCAACCCAAGTCCGGATGACTTAACTGTGAAATGTGTATT
  CTGGGACTTGGGCAGAAATGGTGGCAAAGGAGGTTGGTCATCTGATGGCTGTTCCGTCAAAGACAAGAGA
  ATGAATGAAACCATCTGTACCTGTAGCCATCTTACAAGTTTTGGCATCCTATTGGACCTATCTCGAACAT
  CCTTACCACCAAGTCAAATGATGGCTCTGACATTTATCACGTATATTGGCTGTGGGCTTTCATCAATTTT
  TCTGTCAGTTACTCTTGTAACCTACATCGCCTTTGAAAAGATCCGGAGGGATTACCCCTCCAAAATCCTC
  ATCCAGCTGTGTGCTGCCCTGCTTCTGCTCAACCTGATCTTCCTCCTAGACTCCTGGATTGCGCTGTATA
  ATACCCGAGGTTTCTGCATTGCCGTGGCTGTATTTCTTCACTATTTTCTCTTGGTCTCATTCACATGGAT
  GGGATTAGAAGCATTCCACATGTACCTAGCACTGGTCAAGGTGTTTAATACTTACATCCGAAAGTACATC
  CTTAAATTCTGCATTGTTGGCTGGGGCATACCAGCTGTGGTTGTGTCCATCGTCCTGACTATATCCCCAG
  ATAACTATGGGATTGGATCCTATGGAAAATTCCCCAATGGCACACCAGATGACTTTTGCTGGATCAACAG
  CAATGTGGTGTTCTATATCACGGTTGTGGGATATTTCTGTGTGATATTTCTACTGAACGTCAGCATGTTC
  ATCGTGGTCTTGGTTCAGCTCTGTCGAATTAAAAAGAAGAAGCAGCTGGGAGCCCAGCGCAAAACTAGTA
  TTCAAGACCTCAGGAGTATCGCTGGCCTCACATTTTTACTGGGAATTACTTGGGGCTTTGCCTTCTTTGC
  CTGGGGACCAGTTAATGTCACCTTCATGTATCTCTTTGCCATCTTTAACACCTTACAAGGGTTTTTCATA
  TTCATTTTTTACTGTGCAGCAAAAGAGAATGTCAGAAAACAGTGGAGGCGGTATCTTTGTTGTGGAAAAT
  TACGGCTGGCTGAAAATTCTGACTGGAGTAAAACTGCTACTAATGGTTTAAAGAAGCAGACTGTAAACCA
  AGGAGTATCCAGCTCTTCAAATTCCTTACAGTCAAGCTGTAACTCCACTAACTCCACCACACTCCTAGTG
  AATAGTGATTGCTCAGTGCACGCAAGCGGGAATGGCAATGCATCTACCGAGAGGAATGGGGTTTCTTTCA
  GTGTTCAGAATGGAGACGTGTGCCTTCATGATCTCACTGGCAAACAGCATATGTTTAGTGACAAAGAGGA
  CTCCTGCAATGGTAAAAGCCGGATAGCGCTCAGAAGGACTTCGAAGCGGGGAAGCTTACACTTTATCGAG
  CAAATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222104 representing NM_001079848
  Red=Cloning site Green=Tags(s)
  
  MLFSGGQYSPVGRPEEVLLIYKIFLVIICFHVILVTSLKDAASEVLSTLNKTEKSKITIVKTFNASGVKS
  QRNICNLSSLCNDSVFFRGEIVFQHDEDHNVTQNQDTANGTFAGVLSLSELKRSELNKTLQTLSETYFIV
  CATAEAQSTVNCTFTVKLNETMNVCAMMVTFQTVQIRPMEQCCCSPRTPCPSSPEELEKLQCELQDPIVC
  LADQPHGPPLSSSSKPVVPQATIISHVASDFSLAEPLDHALMTPSTPSLTQESNLPSPQPTIPLASSPAT
  DLPVQSVVVSSLPQTDLSHTLSPVQSSIPSPTTPAPSVPTELVTISTPPGETVVNTSTVSDLEAQVSQME
  KALSLGSLEPNLAGEMVNRVSKLLHSPPALLAPLAQRLLKVVDAIGLQLNFSSTTISLTSPSLALAVIRV
  NASNFNTTTFAAQDPTNLQVSLETPPPENSIGAITLPSSLMNNLPANDVELASRIQFNFFETPALFQDPS
  LENLTLISYVISSSVTNMTIKNLTRNVTVALKHINPSPDDLTVKCVFWDLGRNGGKGGWSSDGCSVKDKR
  MNETICTCSHLTSFGILLDLSRTSLPPSQMMALTFITYIGCGLSSIFLSVTLVTYIAFEKIRRDYPSKIL
  IQLCAALLLLNLIFLLDSWIALYNTRGFCIAVAVFLHYFLLVSFTWMGLEAFHMYLALVKVFNTYIRKYI
  LKFCIVGWGIPAVVVSIVLTISPDNYGIGSYGKFPNGTPDDFCWINSNVVFYITVVGYFCVIFLLNVSMF
  IVVLVQLCRIKKKKQLGAQRKTSIQDLRSIAGLTFLLGITWGFAFFAWGPVNVTFMYLFAIFNTLQGFFI
  FIFYCAAKENVRKQWRRYLCCGKLRLAENSDWSKTATNGLKKQTVNQGVSSSSNSLQSSCNSTNSTTLLV
  NSDCSVHASGNGNASTERNGVSFSVQNGDVCLHDLTGKQHMFSDKEDSCNGKSRIALRRTSKRGSLHFIE
  QM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079848
• ORF Size: 2946 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079848.2, NP_001073317.1
• RefSeq Size: 4646 bp
• RefSeq ORF: 2949 bp
• Locus ID: 237175
• UniProt ID: Q8CJ12
• Cytogenetics: X F4
• Gene Summary: Orphan receptor. Could be involved in a signal transduction pathway controlling epididymal function and male fertility. May regulate fluid exchange within epididymis.[UniProtKB/Swiss-Prot Function]