Prss48 (NM_001001650) Mouse Tagged ORF Clone

CAT#: MG222044

Prss48 (tGFP-tagged) - Mouse protease serine 48 (Prss48), (10ug)

Product Images

Circular map for MG222044

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Prss48
• Synonyms: Esspl; Gm1019
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG222044 representing NM_001001650
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCTGCTGGCCTTAAGGTTCTGCTGCTGCTTTTTCTGGGAGCTTTCCAGGGCTCTTTTACCAAAA
  AGAAAAACTTGCAATCAGTATGTGGACGACCTGTACACACTGGACGCATTGTGGGTGGCCAAGATGCTGC
  TTTGGGGCGCTGGCCCTGGCAGGTGAGCCTGCGTTTTGACTACACCCATTCTTGTGGAGGTTCTCTCATC
  AGTGACCACTGGGTGCTGACGGCAGCACACTGCATAAAAAAGACCTGGTACAGTTTTCTATACAGTGTGT
  GGCTTGGATCCATCGATAGAGAGTACTCAAGCACAGGCAAAGAATACTATGTGTCCAGAATCGCCATCCC
  CGACAAACACCGCCACACAGAAGCAGACATCGCCCTGCTGAAGCTGTCTTCCCGGGTCACCTTCTCATCG
  GTCATTCTGCCCATCTGCTTGCCCAATATCTCAAAGCAGCTGACAGTTCCAGCTTCTTGCTGGGTGACTG
  GATGGGGACAAAATCAGGAAGGTCACTACCCCTCTACCCTCCAAGAACTTGAAGTGCCTGTCATTTCCAG
  TGAGGCGTGTGAACAACTCTACAACCCAATTGGTGTCTTCCTCCCGGATCTGGAGCGGGTCATCAAGGAA
  GACATGTTCTGTGCTGGTGAGAGGCAGTCGAGGAAGGATAGCTGCAAGGGCGATTCTGGAGGGCCTCTGT
  CCTGTCACATTGATGGTGTCTGGAGGCTGATGGGTGTGGTAAGCTGGGGTTTGGAATGCGGTAAGGATCT
  TCCGGGAGTCTACACCAACGTGACCTACTACCAAAAGTGGATCAGTGCTATTATCTCGAGAGCTCCTCCG
  GGCTGGGGTGGTGACAGCACCCACATGACTTCCTGTTCCCTACTATGTTACTTTCTCTGGCTCTCCTGGG
  ATCCTCCCGAGCCTTTGGCCCTAGCG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG222044 representing NM_001001650
  Red=Cloning site Green=Tags(s)
  
  MGPAGLKVLLLLFLGAFQGSFTKKKNLQSVCGRPVHTGRIVGGQDAALGRWPWQVSLRFDYTHSCGGSLI
  SDHWVLTAAHCIKKTWYSFLYSVWLGSIDREYSSTGKEYYVSRIAIPDKHRHTEADIALLKLSSRVTFSS
  VILPICLPNISKQLTVPASCWVTGWGQNQEGHYPSTLQELEVPVISSEACEQLYNPIGVFLPDLERVIKE
  DMFCAGERQSRKDSCKGDSGGPLSCHIDGVWRLMGVVSWGLECGKDLPGVYTNVTYYQKWISAIISRAPP
  GWGGDSTHMTSCSLLCYFLWLSWDPPEPLALA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001650
• ORF Size: 936 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001650.2
• RefSeq Size: 949 bp
• RefSeq ORF: 939 bp
• Locus ID: 368202
• UniProt ID: Q14B25
• Cytogenetics: 3 F1