Hpse (NM_152803) Mouse Tagged ORF Clone

CAT#: MG221855

Hpse (tGFP-tagged) - Mouse heparanase (Hpse), (10ug)

Product Images

Circular map for MG221855

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hpse
• Synonyms: H; Hpa; Hpr1; HSE1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221855 representing NM_152803
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGGCTGCTGCTGCTGTGGCTCTGGGGGCCGCTCGGTGCCCTGGCCCAGGGCGCCCCCGCGGGGA
  CCGCGCCGACCGACGACGTGGTAGACTTGGAGTTTTACACCAAGCGGCCGCTCCGAAGCGTGAGTCCCTC
  GTTCCTGTCCATCACCATCGACGCCAGCCTGGCCACCGACCCGCGCTTCCTCACCTTCCTGGGCTCTCCA
  AGGCTCCGTGCTCTGGCTAGAGGCTTATCTCCTGCATACTTGAGATTTGGCGGCACAAAGACTGACTTCC
  TTATTTTTGATCCGGACAAGGAACCGACTTCCGAAGAAAGAAGTTACTGGAAATCTCAAGTCAACCATGA
  TATTTGCAGGTCTGAGCCGGTCTCTGCTGCGGTGTTGAGGAAACTCCAGGTGGAATGGCCCTTCCAGGAG
  CTGTTGCTGCTCCGAGAGCAGTACCAAAAGGAGTTCAAGAACAGCACCTACTCAAGAAGCTCAGTGGACA
  TGCTCTACAGTTTTGCCAAGTGCTCGGGGTTAGACCTGATCTTTGGTCTAAATGCGTTACTACGAACCCC
  AGACTTACGGTGGAACAGCTCCAACGCCCAGCTTCTCCTTGACTACTGCTCTTCCAAGGGTTATAACATC
  TCCTGGGAACTGGGCAATGAGCCCAACAGTTTCTGGAAGAAAGCTCACATTCTCATCGATGGGTTGCAGT
  TAGGAGAAGACTTTGTGGAGTTGCATAAACTTCTACAAAGGTCAGCTTTCCAAAATGCAAAACTCTATGG
  TCCTGACATCGGTCAGCCTCGAGGGAAGACAGTTAAACTGCTGAGGAGTTTCCTGAAGGCTGGCGGAGAA
  GTGATCGACTCTCTTACATGGCATCACTATTACTTGAATGGACGCATCGCTACCAAAGAAGATTTTCTGA
  GCTCTGATGTGCTGGACACTTTTATTCTCTCTGTGCAAAAAATTCTGAAGGTCACTAAAGAGATCACACC
  TGGCAAGAAGGTCTGGTTGGGAGAGACGAGCTCAGCTTACGGTGGCGGTGCACCCTTGCTGTCCAACACC
  TTTGCAGCTGGCTTTATGTGGCTGGATAAATTGGGCCTGTCAGCCCAGATGGGCATAGAAGTCGTGATGA
  GGCAGGTGTTCTTCGGAGCAGGCAACTACCACTTAGTGGATGAAAACTTTGAGCCTTTACCTGATTACTG
  GCTCTCTCTTCTGTTCAAGAAACTGGTAGGTCCCAGGGTGTTACTGTCAAGAGTGAAAGGCCCAGACAGG
  AGCAAACTCCGAGTGTATCTCCACTGCACTAACGTCTATCACCCACGATATCAGGAAGGAGATCTAACTC
  TGTATGTCCTGAACCTCCATAATGTCACCAAGCACTTGAAGGTACCGCCTCCGTTGTTCAGGAAACCAGT
  GGATACGTACCTTCTGAAGCCTTCGGGGCCGGATGGATTACTTTCCAAATCTGTCCAACTGAACGGTCAA
  ATTCTGAAGATGGTGGATGAGCAGACCCTGCCAGCTTTGACAGAAAAACCTCTCCCCGCAGGAAGTGCAC
  TAAGCCTGCCTGCCTTTTCCTATGGTTTTTTTGTCATAAGAAATGCCAAAATCGCTGCTTGTATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221855 representing NM_152803
  Red=Cloning site Green=Tags(s)
  
  MLRLLLLWLWGPLGALAQGAPAGTAPTDDVVDLEFYTKRPLRSVSPSFLSITIDASLATDPRFLTFLGSP
  RLRALARGLSPAYLRFGGTKTDFLIFDPDKEPTSEERSYWKSQVNHDICRSEPVSAAVLRKLQVEWPFQE
  LLLLREQYQKEFKNSTYSRSSVDMLYSFAKCSGLDLIFGLNALLRTPDLRWNSSNAQLLLDYCSSKGYNI
  SWELGNEPNSFWKKAHILIDGLQLGEDFVELHKLLQRSAFQNAKLYGPDIGQPRGKTVKLLRSFLKAGGE
  VIDSLTWHHYYLNGRIATKEDFLSSDVLDTFILSVQKILKVTKEITPGKKVWLGETSSAYGGGAPLLSNT
  FAAGFMWLDKLGLSAQMGIEVVMRQVFFGAGNYHLVDENFEPLPDYWLSLLFKKLVGPRVLLSRVKGPDR
  SKLRVYLHCTNVYHPRYQEGDLTLYVLNLHNVTKHLKVPPPLFRKPVDTYLLKPSGPDGLLSKSVQLNGQ
  ILKMVDEQTLPALTEKPLPAGSALSLPAFSYGFFVIRNAKIAACI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152803
• ORF Size: 1605 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152803.5
• RefSeq Size: 3082 bp
• RefSeq ORF: 1608 bp
• Locus ID: 15442
• UniProt ID: Q6YGZ1
• Cytogenetics: 5 E4
• Gene Summary: This gene encodes an endoglucuronidase enzyme that plays an important role in tumor invasion and metastasis. The encoded preproprotein undergoes proteolytic processing to generate an active heterodimeric enzyme that cleaves the heparan sulfate proteoglycans associated with the cell surface and extracellular matrix. Mice lacking the encoded protein do not show any prominent pathological alterations under normal conditions but fail to develop albuminuria and renal damage in response to drug-induced diabetes. [provided by RefSeq, Aug 2016]