Tsks (NM_011651) Mouse Tagged ORF Clone

CAT#: MG221043

Tsks (tGFP-tagged) - Mouse testis-specific serine kinase substrate (Tsks) transcript variant 1, (10ug)

Product Images

Circular map for MG221043

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tsks
• Synonyms: Stk22; Stk22s1; Ts; Tss; Tssks1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG221043 representing NM_011651
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAGCGTGGTGGTGAAGACAATCTGGCAATCCAAAGAGATCCACGAAGCGGGGGACCCACCTGCGG
  GGGTAGAAAGCCGTGCCCAGCTGGTCCCCGAGGCTCCCGGGGGGGTGACCAGCCCTGCCAAAGGGATAAC
  GAAAAAAAAGAAGGCTGTGTCCTTCCATGGGGTGGAGCCCCGGATGTCCCACGAGCCGATGCACTGGTGC
  CTGAACCTCAAGCGGTCCTCTGCCTGCACCAACGTGTCCTTGCTCAACCTGGCTGCCGTGGAGCCCGACT
  CCTCGGGCACAGACTCGACCACAGAGGACAGTGGTCCACTGGCACTGCCAGGGCCACCTGCTTCCCCTAC
  AACACCCTGGGCTCCAGAGGACCCTGACATCACAGAACTACTGAGTGGGGTCAACAGTGGATTGGTACGT
  GCCAAAGACTCCATCACCAGCTTGAAGGAAAAGACCACGCGGGTTAATCAGCACGTTCAGACTCTGCAGA
  GCGAGTGCTCTGTGCTGAGTGAGAATCTGGAAAGAAGACGGCAGGAGGCAGAAGAGTTGGAGGGGTACTG
  CAGTCAGTTGAAGGGCCCCCGCCCTGATGTCCTGACCCAGGAGAACTGCCGCAAGGTGACCCGTTCAGTG
  GAAGACGCTGAAATCAAAACCAATGTCCTGAAGCAGAACTCTGCCTTGCTGGAGGAGAAGCTAAGATACC
  TCCAGCAGCAACTGCAGGATGAGACGCCCCGGAGACAGGAGGCCGAGTTGCAGGAGTTGGAGCAAAAACT
  GGAGGCTGGCCTCTCCCGACATGGTCTGAGCCCTGCCACTCCCATTCAGGGCTGCTCGGGTCCTCCTGGC
  AGCCCCGAGGAACCCCCGCGGCAGCGAGGCCTGTCCTCCAGTGGCTGGGGCATGGCAGTCCGCACAGGGG
  AGGGACCCTCGCTGAGCGAGCAGGAGTTGCAGAAGGTCTCCACCGGCCTGGAGGAGCTGAGGAGGGAGGT
  GTCCTCGCTGGCAGCCCGGTGGCATCAGGAGGAGGGGGCAGTGCAGGAGGCCCTGAGGTTGCTGGGAGGC
  CTTGGCGGCCGTCTGGATGGCTTCCTGGGCCAGTGGGAGCGGGCGCAGCGGGAACAGGCACAGTCCGCAA
  GGGGCTTGCAGGAGCTGCGAGGACGAGCAGATGAGTTGTGCACTATGGTGGAGAGGTCAGCAGTGTCTGT
  CGCCTCACTGAGGAGTGAACTGGAGGCACTGGGCCCAGTAAAACCCATTCTGGAGGAGCTGGGACGGCAA
  CTTCAGAACTCCCGGAGGGGAGCTGACCATGTCTTGAACTTGGATCGGTCTGCCCAAGGCCCCTGTGCGC
  GCTGTGCCAGCCAGGGGCAGCAGTTATCCACGGAGTCCCTGCAGCAGCTGCTGGAACGTGCGCTGACCCC
  GCTGGTGGATGAGGTGAAGCAGAAGGGTCTGGCTCCTGCCTGCCCCAGTTGCCAGAGGCTACACAAGAAG
  ATTCTGGAGCTGGAGCGCCAGGCCTTGGCCAAACACGTCAGGGCAGAGGCCCTGAGCTCCACCCTTCGGT
  TGGCCCAAGACGAGGCCGTTCGGGCCAAGAACCTACTGCTGACGGACAAGATGAAGCCGGAGGAGAAGGT
  GGCCACTTTGGACTATATGCATTTGAAGATGTGCTCCCTCCACGACCAACTCAGCCACCTGCCACTTGAG
  GGGTCCACGGGGGCAATGGGGGGAGGAAGCAATGGAGGGGCTCCCCCTAAACGTGGGAGTCCAGGCTCTG
  AACAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG221043 representing NM_011651
  Red=Cloning site Green=Tags(s)
  
  MASVVVKTIWQSKEIHEAGDPPAGVESRAQLVPEAPGGVTSPAKGITKKKKAVSFHGVEPRMSHEPMHWC
  LNLKRSSACTNVSLLNLAAVEPDSSGTDSTTEDSGPLALPGPPASPTTPWAPEDPDITELLSGVNSGLVR
  AKDSITSLKEKTTRVNQHVQTLQSECSVLSENLERRRQEAEELEGYCSQLKGPRPDVLTQENCRKVTRSV
  EDAEIKTNVLKQNSALLEEKLRYLQQQLQDETPRRQEAELQELEQKLEAGLSRHGLSPATPIQGCSGPPG
  SPEEPPRQRGLSSSGWGMAVRTGEGPSLSEQELQKVSTGLEELRREVSSLAARWHQEEGAVQEALRLLGG
  LGGRLDGFLGQWERAQREQAQSARGLQELRGRADELCTMVERSAVSVASLRSELEALGPVKPILEELGRQ
  LQNSRRGADHVLNLDRSAQGPCARCASQGQQLSTESLQQLLERALTPLVDEVKQKGLAPACPSCQRLHKK
  ILELERQALAKHVRAEALSSTLRLAQDEAVRAKNLLLTDKMKPEEKVATLDYMHLKMCSLHDQLSHLPLE
  GSTGAMGGGSNGGAPPKRGSPGSEQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011651
• ORF Size: 1755 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011651.2, NP_035781.2
• RefSeq Size: 1828 bp
• RefSeq ORF: 1758 bp
• Locus ID: 22116
• UniProt ID: O54887
• Cytogenetics: 7 B3
• Gene Summary: This gene is a member of a small family of testis-specific serine/threonine kinases. This gene may play a role in testicular physiology or spermiogenesis. Its expression is restricted to the testis during the last stages of spermatid maturation. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2008]