Slc9a9 (NM_177909) Mouse Tagged ORF Clone

CAT#: MG220838

Slc9a9 (tGFP-tagged) - Mouse solute carrier family 9 (sodium/hydrogen exchanger) member 9 (Slc9a9), (10ug)

Product Images

Circular map for MG220838

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Slc9a9
• Synonyms: 5730527A11Rik; 9930105B05; AI854429; Nhe9
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220838 representing NM_177909
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGGCAGCTTCGGTTTACGTCAGGGAAGGATGAAGATCATTTTCAACACCAGGGAGCAGTGGAGC
  TACTCGCGTTTAATTTTTTGCTCATCCTTACCATTTTAACAATCTGGTTATTTAAAAATCACCGATTCCG
  CTTCTTGCATGAGACTGGAGGGGCGATGGTGTATGGCCTCATAATGGGACTAATTTTGCGATATGCCACA
  GCGCCGACTGATATTGATAGTGGAACTGTCTACAACTGTGGGAACTTGTTCTTCAGCCCATCAACTCTAC
  TAGTCAACATCACCGACCAAGTTTATGAGTATAAGTACCAGAGAGAGATCAACCAGCACAATATCAGCCC
  CCACCAAGGCAACGCCATACTGGAGAAGATGACGTTTGACCCAGAAATCTTCTTCAACGTTTTGCTGCCT
  CCCATCATATTTCATGCAGGATACAGTCTGAGGAAGAGACACTTTTTTCAAAACTTGGGATCCATTTTAA
  CGTATGCCTTCTTGGGAACAGCCATCTCCTGTGTGGTCATAGGGTTAATTATGTACGGCTTTGTGAAAGC
  CATGGTGCATGCTGGCCAGCTGAAGAGCGGAGACTTCCACTTCACTGACTGTTTATTTTTTGGTTCACTG
  ATGTCTGCTACAGATCCAGTGACAGTGCTGGCCATTTTCCATGAACTCCACGTGGACCCAGACCTGTACA
  CACTGCTGTTTGGAGAGAGTGTACTGAATGATGCGGTGGCCATAGTCCTCACATACTCTATATCCATCTA
  TAGTCCCAAGGAGAATCCCAACGCGTTTGACACGGCAGCGTTCTTCCAATCCGTGGGCAATTTCCTAGGG
  ATCTTTGCCGGCTCCTTTGCAATGGGCTCCGCATATGCAGTTGTTACCGCACTGTTGACCAAATTTACCA
  AGCTCCGTGAGTTCCCGATGCTGGAGACAGGTCTGTTTTTCCTCCTTTCTTGGAGTGCCTTCCTGTCTGC
  AGAGGCCGCCGGCTTAACAGGCATAGTTGCTGTTCTCTTCTGTGGAGTCACACAGGCACATTACACCTAC
  AACAATCTGTCATCGGATTCCAAACTGAGGACCAAACAGCTGTTTGAATTTATGAACTTCCTGGCTGAAA
  ACGTCATCTTCTGCTACATGGGCCTGGCTCTGTTCACCTTCCAGAATCACATTTTCAATGCTCTTTTTAT
  ACTCGGAGCCTTTCTAGCAATTTTTGTTGCCAGAGCCTGCAACATTTACCCCCTCTCCTTCCTCCTGAAT
  CTGGGCAGAAAGCAGAAGATTCCCTGGAACTTCCAGCACATGATGATGTTTTCAGGTTTGCGAGGTGCCA
  TTGCCTTCGCCCTGGCCATCCGCAACACGGAATCTCAGCCCAAACAGATGATGTTCACCACCACACTGCT
  CCTTGTGTTCTTCACTGTCTGGGTGTTTGGAGGAGGAACGACCCCCATGCTGACGTGGCTTCAGATCAGA
  GTTGGTGTGGACCTGGATGAAAGTCTGAAGGAAGAGCCCTCCTCACAGCAGGAAGCAAATAAGTTGGACA
  AAAACATGACGAAGACAGAGAGTGCACAGCTCTTCAGAATGTGGTATGGATTTGACCACAAATACCTGAA
  ACCCATCCTAACACACTCAGGCCCTCCACTGACCACCACCTTGCCAGCGTGGTGCGGCCCAGTCTCCAGG
  CTGCTCACCAGTCCGCAGGCCTATGGGGAACAACTGAAAGAGGATGACGTGGAATGTATCGTCAACCAAG
  ATGAACTGGCCATGAATTACCAGGAGCAGAGCCCTTCACCCAGCAGTCCCACCACCAAGCTAGCTCTGGA
  CCAGAAGTCTTCAGGCCAGACTCCAGGCAAGGAGAATATCTACGAGGGAGATCTCGGCCTGGGAGGCTAT
  GATCTCAAACTGGAGCAGACTCGGGGTCAACCCCAGATGGAC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220838 representing NM_177909
  Red=Cloning site Green=Tags(s)
  
  MAGQLRFTSGKDEDHFQHQGAVELLAFNFLLILTILTIWLFKNHRFRFLHETGGAMVYGLIMGLILRYAT
  APTDIDSGTVYNCGNLFFSPSTLLVNITDQVYEYKYQREINQHNISPHQGNAILEKMTFDPEIFFNVLLP
  PIIFHAGYSLRKRHFFQNLGSILTYAFLGTAISCVVIGLIMYGFVKAMVHAGQLKSGDFHFTDCLFFGSL
  MSATDPVTVLAIFHELHVDPDLYTLLFGESVLNDAVAIVLTYSISIYSPKENPNAFDTAAFFQSVGNFLG
  IFAGSFAMGSAYAVVTALLTKFTKLREFPMLETGLFFLLSWSAFLSAEAAGLTGIVAVLFCGVTQAHYTY
  NNLSSDSKLRTKQLFEFMNFLAENVIFCYMGLALFTFQNHIFNALFILGAFLAIFVARACNIYPLSFLLN
  LGRKQKIPWNFQHMMMFSGLRGAIAFALAIRNTESQPKQMMFTTTLLLVFFTVWVFGGGTTPMLTWLQIR
  VGVDLDESLKEEPSSQQEANKLDKNMTKTESAQLFRMWYGFDHKYLKPILTHSGPPLTTTLPAWCGPVSR
  LLTSPQAYGEQLKEDDVECIVNQDELAMNYQEQSPSPSSPTTKLALDQKSSGQTPGKENIYEGDLGLGGY
  DLKLEQTRGQPQMD
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177909
• ORF Size: 1932 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177909.3
• RefSeq Size: 3492 bp
• RefSeq ORF: 1935 bp
• Locus ID: 331004
• UniProt ID: Q8BZ00
• Cytogenetics: 9 E3.3
• Gene Summary: May act in electroneutral exchange of protons for Na(+) across membranes. Involved in the effusion of Golgi luminal H(+) in exchange for cytosolic cations. Involved in organelle ion homeostasis by contributing to the maintenance of the unique acidic pH values of the Golgi and post-Golgi compartments in the cell (By similarity).[UniProtKB/Swiss-Prot Function]