Chordc1 (NM_025844) Mouse Tagged ORF Clone

CAT#: MG220787

Chordc1 (tGFP-tagged) - Mouse cysteine and histidine-rich domain (CHORD)-containing zinc-binding protein 1 (Chordc1), (10ug)

Product Images

Circular map for MG220787

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Chordc1
• Synonyms: 1110001O09Rik; AA409036; Chp-1; morgana
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220787 representing NM_025844
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTCTTGTGTTACAACCGCGGCTGTGGCCAGCGTTTCGACCCAGAGGCCAACTCGGACGATGCTT
  GCACATACCACCCAGGTGTTCCTGTCTTTCATGATGCCCTAAAGGGTTGGTCTTGCTGTAAAAGAAGGAC
  AACTGATTTCTCTGACTTCTTAAGCATTGTAGGCTGTACAAAAGGTAGGCATAATAGTGAGAAGCCACCT
  GAACCGGTCAAGCCTGAAGTCAAGACTACTGAGAAGAAGGAACTATCTGAATTAAAACCCAAATTTCAGG
  AGCACATCATTCAAGCCCCTAAGCCAGTAGAAGCAATAAAAAGGCCAAGCCCAGATGAGCCAATGACAAA
  CTTGGAGTTAAAAATATCTGCTTCCCTAAAACAAGCACTTGATAAACTTAAACTGTCATCTGGGAGTGAA
  GAAGATAAAAAAGAAGAAGACAGTGATGAAATTAAGATTGGGACTTCCTGTAAAAATGGAGGGTGTTCAA
  AGACATACCAGGGTCTACAGAGTCTAGAAGAAGTCTGTGTATATCATTCTGGAGTACCTATTTTCCACGA
  GGGGATGAAATATTGGAGCTGTTGTAGAAGAAAAACTTCAGATTTTAATACATTCTTAGCCCAAGAGGGC
  TGTACAAGAGGGAAGCATGTATGGACTAAAAAAGATGCAGGGAAAAAAGTTGTTCCGTGTAGACACGACT
  GGCATCAGACTGGGGGTGAAGTTACCATCTCAGTGTATGCTAAAAATTCCCTGCCAGAACTCAGTCAAGT
  AGAAGCCAACAGTACTTTGTTAAATGTACACATTGTATTTGAAGGAGAGAAAGAATTTCATCAAAATGTG
  AAACTGTGGGGTGTAATTGATGTTAAACGAAGTTATGTAACTATGACTGCAACAAAGATTGAGATCACTA
  TGAGAAAGGCGGAACCCATGCAGTGGGCAAGTCTTGAACTACCTACAACCAAAAAGCAGGAGAAACAAAA
  AGATATAGCTGAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220787 representing NM_025844
  Red=Cloning site Green=Tags(s)
  
  MALLCYNRGCGQRFDPEANSDDACTYHPGVPVFHDALKGWSCCKRRTTDFSDFLSIVGCTKGRHNSEKPP
  EPVKPEVKTTEKKELSELKPKFQEHIIQAPKPVEAIKRPSPDEPMTNLELKISASLKQALDKLKLSSGSE
  EDKKEEDSDEIKIGTSCKNGGCSKTYQGLQSLEEVCVYHSGVPIFHEGMKYWSCCRRKTSDFNTFLAQEG
  CTRGKHVWTKKDAGKKVVPCRHDWHQTGGEVTISVYAKNSLPELSQVEANSTLLNVHIVFEGEKEFHQNV
  KLWGVIDVKRSYVTMTATKIEITMRKAEPMQWASLELPTTKKQEKQKDIAD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025844
• ORF Size: 993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025844.2, NP_080120.1
• RefSeq Size: 2205 bp
• RefSeq ORF: 996 bp
• Locus ID: 66917
• UniProt ID: Q9D1P4
• Cytogenetics: 9 A2
• Gene Summary: Regulates centrosome duplication, probably by inhibiting the kinase activity of ROCK2. Proposed to act as co-chaperone for HSP90. May play a role in the regulation of NOD1 via a HSP90 chaperone complex. In vitro, has intrinsic chaperone activity. This function may be achieved by inhibiting association of ROCK2 with NPM1. Involved in stress response. Prevents tumorigenesis (By similarity).[UniProtKB/Swiss-Prot Function]