Ccdc110 (NM_001033246) Mouse Tagged ORF Clone

CAT#: MG220321

Ccdc110 (tGFP-tagged) - Mouse coiled-coil domain containing 110 (Ccdc110), (10ug)

Product Images

Circular map for MG220321

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ccdc110
• Synonyms: Gm172
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG220321 representing NM_001033246
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCCCGGCTACCTTCCAGCTCCAAAAGCTGTGCTCGGGTCTGTTCCAGAGAAGCACCTCGCGGAAG
  AAGATGAAGTTGACTCCATTCTCCTTTCAGCATCCAAGATCCTGAATTCCTCTGAGGGGGTAAAGGAAAG
  CGGGGGCAATGAACCAGAATATGGCTGTGCGTCAGAACCAGAAAATCAAATCCAACCACAATCAGCACTG
  AAAGTCCTACAACATCAGCTGGAGTCGTTTCAGGCTCTGCGGATGCAGACTCTACAGAACGTCAGCATGG
  TACAGTCTGAAATCAGTGAAATACTGAACAAAAGTATTGTTGAAGTAGAAACCCCACAATTTAACTCGGA
  AAAAAGTCTAGTATTCAGCATGCATCCTGAAAAGGATTTGCCTAACGAGACACAAGAAGAAATACCTTCC
  ACCAAGACTCTCCATTCGATGGGAGAGACCTTCAGTAGTAACAGTGACACTGGTCTCCCTCAGGGCACAG
  ATATTCCACCCCAGATTCAGGTCAAGGACATGCTAGCTCTGCAGGGCCTGAGAACTACCGCTGATAACTC
  ACCTCCCAAGAAAGCTATGAACACTTCGGAGCAGCCCAGCGCCACAAAGAGTTTCAGCCTCTATCAGTTT
  CTACCTCAGGGTCCTCAAACAGCGGTGCCCCAAGCTGCTCCCGTGATTCTGGATAAATCCACCATCACCA
  CCCCTTTCCCAAAGCATGGGTTCTGTGCAAACTTAGACGACATCTGCCACTCGATCAAGCACATGAAAGA
  GGAGCTTCAGAAGTCACACGACAAGGAGCTGGCACTCACAAGTGAACTCCACACTTTCCAGGCAGACGCG
  AGTACTCAAGGTCACCACAAACATGAGCCGTTTCCCATGCATAGCTCAAAACTTAATTTTATCCAGCAAG
  AAAACATGGAAGGTAACTTAAGTGAAGACATGAAATCGAAGAGAATTTCCGAATTAGAGGCCTTAGTGAG
  CAAACTGCTCCCACTCAGGGACACGGTGTCAAAACTGCATGTGAACTTTTGTAGGAAATGTAAAAAGTTA
  TCTAAAAGTGAAGTATACCGTGGGAAGAAGAATGAGAAAAATAAAGACATCCCTATCACCAGCAAGAACA
  TTATGGATTTAAAGTTCCACTCCCGAGCTCCAAGGTACACTCTGTCGGTCCTGGACCTAGGGAAACACAA
  AATAAAAGACAAAGAAGGCCAAGCGTTCATAGTCAGCCAAGGACCCGCAACGCTTGAAAACGAGAAGTTA
  CCCAAAGGCAAGTTCATCCCAGAGCAGTGCGTCCCCAAGCAGAGCGTCGCCAAAATTCATTACCTACAGA
  ATTACCTAAAAGAATCCTTGCAGAATCAGAAAAAGATGACCGAACTGGAGAACGAAAACCTAGCCCTTAA
  GACCAAAATGAAGCCCCTTGTGTTCACCGCACAATCCCTGATCCAGAAGGTGGAAGCCCACGAGAAGCAA
  CTGAAGTCTCTGGCTGAAGAAAAGAGTGCTCTTCAGTCCAAGTTAAGCAAAGCAGAGGAAGAGAACAAAG
  ACTGCCTCAGGGAACTTAAGAAAATCGTCAGCACTTACAACGTCCTCGGAGGCCAACACAAAATGCTAGA
  GGAGAAAAATAGTCAGCTCTCTCTGGAAAAGCAACAGATGCTGGAAACGATAGATCACCTGAAGAGCAAG
  GAACACAAGTCCCAAAGTGACATGGCCGTCCTCCAAAACGAAAACAGTAGAATGAACATAGAAATAGAAG
  CCATGAAAACAAGCATGCTGTTGGTACAAGACGAAAGGGAAATGCTAGAGAAAGAGACGTACCAGCTCCT
  GAAAGACAAGAGCACGCTCGAGAGTGACCTGAAAGAGAGCAAGCTGGAGATCCTGCAGCTGAAAGAAAAG
  GAGAGACTGATTAAAGCGGAGCAGGAGTCGCTTCTTCACAGTCTAGACACAGCTAAAGCGGAGAAGCTGA
  GTCTGGAGGCGACACTGCAGGAGTCCACTTCCACCAGACAGAAGTTGGAAAGGAAGCTCGTGGATATTCA
  AGCCTACCAGTCAGCCGCGGAGGAGAAGTTCCTGAAAGAAATCAAGAGTGCAAAGTCGGAGACAAGCATC
  TACAAAAACAACTTGGCCGAAATCAGCAAGGAGTGTGAGATCTTGTCCAAAATGGTCATGGAGATTAAGG
  CGGATAACCAGATTCTGAAAGAAGAACTCAAGAAACACAGTCAAGAAAACACCAAGTTCGAAAATAGCAT
  CAGTCGGCTCACCGAAGACAAGATACTTCTGGAAAACTACGTGAGAAGCATAGAAAATGAGAAAGACACC
  CTGGAATTTGAGATGCGGAATCTTCAGCGAGACTACTTGAGTTTAAGTGATAAAGTCTCCAGTCAGAACA
  ACAGCGCATCAAAATCAACCTATATTTCAAGACGAGAAAAACTCTACTTCGATAACTACGATGCCTATGA
  AGATGCTTCCAGTCTTAGGAATCGGCCTGTGGCTTCTGACTTGAAAGGAATACCACATAAACTGTATCAA
  CGGCTTCCATCCAAGATATGTAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG220321 representing NM_001033246
  Red=Cloning site Green=Tags(s)
  
  MSPGYLPAPKAVLGSVPEKHLAEEDEVDSILLSASKILNSSEGVKESGGNEPEYGCASEPENQIQPQSAL
  KVLQHQLESFQALRMQTLQNVSMVQSEISEILNKSIVEVETPQFNSEKSLVFSMHPEKDLPNETQEEIPS
  TKTLHSMGETFSSNSDTGLPQGTDIPPQIQVKDMLALQGLRTTADNSPPKKAMNTSEQPSATKSFSLYQF
  LPQGPQTAVPQAAPVILDKSTITTPFPKHGFCANLDDICHSIKHMKEELQKSHDKELALTSELHTFQADA
  STQGHHKHEPFPMHSSKLNFIQQENMEGNLSEDMKSKRISELEALVSKLLPLRDTVSKLHVNFCRKCKKL
  SKSEVYRGKKNEKNKDIPITSKNIMDLKFHSRAPRYTLSVLDLGKHKIKDKEGQAFIVSQGPATLENEKL
  PKGKFIPEQCVPKQSVAKIHYLQNYLKESLQNQKKMTELENENLALKTKMKPLVFTAQSLIQKVEAHEKQ
  LKSLAEEKSALQSKLSKAEEENKDCLRELKKIVSTYNVLGGQHKMLEEKNSQLSLEKQQMLETIDHLKSK
  EHKSQSDMAVLQNENSRMNIEIEAMKTSMLLVQDEREMLEKETYQLLKDKSTLESDLKESKLEILQLKEK
  ERLIKAEQESLLHSLDTAKAEKLSLEATLQESTSTRQKLERKLVDIQAYQSAAEEKFLKEIKSAKSETSI
  YKNNLAEISKECEILSKMVMEIKADNQILKEELKKHSQENTKFENSISRLTEDKILLENYVRSIENEKDT
  LEFEMRNLQRDYLSLSDKVSSQNNSASKSTYISRREKLYFDNYDAYEDASSLRNRPVASDLKGIPHKLYQ
  RLPSKICK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033246
• ORF Size: 2544 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033246.3
• RefSeq Size: 2626 bp
• RefSeq ORF: 2547 bp
• Locus ID: 212392
• UniProt ID: Q3V125
• Cytogenetics: 8 B1.1