Cnksr3 (NM_172546) Mouse Tagged ORF Clone

CAT#: MG219948

Cnksr3 (tGFP-tagged) - Mouse Cnksr family member 3 (Cnksr3), (10ug)

Product Images

Circular map for MG219948

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cnksr3
• Synonyms: 6820402C05; BC024086; Magi1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219948 representing NM_172546
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGTGACCAAGTGGAGCCCCAAACAAGTGGTGGACTGGACTAGAGGGTTGGATGACTGCCTGC
  AGCCATATGTGCACAAGTTTGAGCGGGAGAAGATAGACGGGGAGCAGTTGCTGAAAATCTCCCACCAGGA
  TCTCGAGGAACTGGGGGTCACACGGATCGGCCACCAAGAGCTGGTGTTGGAGGCTGTGGATCTTCTCTGT
  GCACTGAATTATGGCCTTGAAACTGATACTATGAAGAACTTGGTTCTGAAACTGCGGGCATCTTCCCACA
  ACTTGCAGAATTACATCAGTAGCCGCCGGAAGAGTCCAGCCTACGATGGGAATACGTCCCGTAAGCCCCC
  CAACGAGTTCCTGACTTCTGTGGTGGAACTCATTGGTGCTGCCAAGGCCCTGCTGGCTTGGCTGGACCGG
  GCTCCATTCACAGGGATCACAGATCTCTCTGTGACAAAGAACAAAATCATCCAACTGTGCCTGGACCTGA
  CCACAGCAGTCCAGAAGGATTGCCTCATAGCAGAAATGGAGGATAAAGTTTTAAATGTGGTCAAGGTTTT
  AAATGGCATCTGTGACAAAACAATGCGCTCTACTACAGACCCTGTTATGAGCCAGTGTGCCTGCCTGGAG
  GAAGTTCACTTACCAAATGTTAGACCCGGGGAAGGCCTGGGCATGTACATCAAGTCAACCTACGACGGAC
  TGCATGTGATTACTGGAACCACAGAAAATTCTCCTGCAGACAGATCTCAAAAGATTCACGCTGGCGATGA
  GGTCATTCAAGTCAATCGGCAGACAGTGGTTGGGTGGCAGTTGAAAAATCTGGTGAGAAAGTTGAGAGAG
  AACCCCACGGGCGTGGTGCTGCTGCTGAAGAAGAGGCCCACGGGCTCCTTCAGCTTCACTCCGGCCCCAC
  TGAAGAACCTGCGGTGGAAGCCACCGCTCGTGCAGACCTCACCCCCGCCCACCACCACCCAGTCCCCAGA
  GAGTACCATGGATGCCTCCCTGAAGAAGGAGAAGCCAGCCATCCTGGATCTATACATCCCTCCCCCACCT
  GCTGTCCCCTACTCTCCCCGGGATGAGAATGTGAGTTTCGGCTACAGAGGACACAGTAAGAGTAAGCAGC
  CGTTGCCTGTGCGCAAGGGTTCCGAGTCCCCTAACTCCTTCCTGGACCAGGAGAGCCAAAGACGCAGATT
  TACCATTGCAGATTCCGATCAGCTGCCCGGGTATTCGGTGGAAACCAATGTTCTGCCCACAAAAATGAGA
  GGGAAGACACCATCCTACGGCAAACCCCGGCCTCTGTCCATGCCTGCCGATGGGAACTGGATGGGGATCG
  TGGACCCTTTTGCTAAACCTCGAGGAAATGGGAGGAAAGGGGAAGATGCCCTCTGCCGGTACTTCAGCAA
  TGAGCGGATCACACCCATCACTGAAGAAAGTGCCTCTCCTATGTACCGGTTCTCTAGACCCCTGACTGAG
  CGGCACCTGGTCCGAGGTGCGGACTACATCCGAGGGAGCAGGTGCTACATCAACTCCGACCTACACAGCA
  GTGCCACCATTCCATTCCAGGAGGAAGGTTCCAAGAAGAAATCAGCCTCCTCATCGGCCAAGGCGTCCTC
  TGGGGAGCCGTCGCTGCTGGTCAGCTGGCTGACTCGCCTCAAGCTGTTGACTCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219948 representing NM_172546
  Red=Cloning site Green=Tags(s)
  
  MEPVTKWSPKQVVDWTRGLDDCLQPYVHKFEREKIDGEQLLKISHQDLEELGVTRIGHQELVLEAVDLLC
  ALNYGLETDTMKNLVLKLRASSHNLQNYISSRRKSPAYDGNTSRKPPNEFLTSVVELIGAAKALLAWLDR
  APFTGITDLSVTKNKIIQLCLDLTTAVQKDCLIAEMEDKVLNVVKVLNGICDKTMRSTTDPVMSQCACLE
  EVHLPNVRPGEGLGMYIKSTYDGLHVITGTTENSPADRSQKIHAGDEVIQVNRQTVVGWQLKNLVRKLRE
  NPTGVVLLLKKRPTGSFSFTPAPLKNLRWKPPLVQTSPPPTTTQSPESTMDASLKKEKPAILDLYIPPPP
  AVPYSPRDENVSFGYRGHSKSKQPLPVRKGSESPNSFLDQESQRRRFTIADSDQLPGYSVETNVLPTKMR
  GKTPSYGKPRPLSMPADGNWMGIVDPFAKPRGNGRKGEDALCRYFSNERITPITEESASPMYRFSRPLTE
  RHLVRGADYIRGSRCYINSDLHSSATIPFQEEGSKKKSASSSAKASSGEPSLLVSWLTRLKLLTH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172546
• ORF Size: 1665 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172546.2, NP_766134.1
• RefSeq Size: 3448 bp
• RefSeq ORF: 1668 bp
• Locus ID: 215748
• UniProt ID: Q8BMA3
• Cytogenetics: 10 A1
• Gene Summary: Involved in transepithelial sodium transport. Regulates aldosterone-induced and epithelial sodium channel (ENaC)-mediated sodium transport through regulation of ENaC cell surface expression. Acts as a scaffold protein coordinating the assembly of an ENaC-regulatory complex (ERC).[UniProtKB/Swiss-Prot Function]