Actbl2 (NM_175497) Mouse Tagged ORF Clone

CAT#: MG219887

Actbl2 (tGFP-tagged) - Mouse actin beta-like 2 (Actbl2), (10ug)

Product Images

Circular map for MG219887

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Actbl2
• Synonyms: 4732495G21Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219887 representing NM_175497
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTGACGATGAGCTGACTGCTTTGGTAGTAGATAATGGGTCAGGGATGTGCAAGGCAGGTTTTGGGG
  GTGACGATGCCCCCCGTGCTGTGTTCCCCTCCATGGTTGGTCGTCCACGACACCAGGGGGTCATGGTAGG
  CATGGGCCAGAAGGACTGTTATGTGGGAGATGAGGCCCAGAGCAAGCGAGGCATCTTGACCTTGAAGTAT
  CCCATTGAACACGGAGTCGTCACCAACTGGGATGATATGGAGAAGATCTGGTACCACACTTTCTACAATG
  AGCTCCGGGTAGCACCGGATGAACATCCTATTCTTCTCACCGAGGCACCCCTTAACCCCAAAATCAACCG
  GGAAAAGATGACTCAGATAATGTTCGAGGCTTTCAACACACCTGCCATGTATGTAGCAATCCAAGCCGTG
  CTATCCCTCTATGCTTCCGGAAGGACTACAGGTATCGTGATGGATTCTGGGGATGGGGTCACTCACACTG
  TGCCTATCTATGAAGGTTACGCTCTGCCTCATGCCATTCTACGACTAGATCTGGCAGGTAGGGACCTGAC
  TGATTACCTCATGAAGATTCTGACAGAACGAGGCTATAACTTCACCACCACAGCAGAGAGGGAGATCGTG
  CGGGATGTCAAAGAAAAGTTGTGCTACGTGGCCCTGGACTTTGAGCAGGAGATGGTCACTGCAGCCGCTT
  CATCCTCACTAGAGAGAAGCTATGAGCTCCCTGATGGGCAGGTGATCACCATTGGAAATGAACGCTTCCG
  GTGCCCAGAAGCCATTTTCCAGCCTTCCTTTCTAGGCATTGAATCCAGAGGCATCCATGAGACAACCTTC
  AACTCGATCATGAAGTGTGATGTAGATATTCGTAAGGATTTATTTGCCAACACTGTGCTGTCTGGAGGCA
  GTACCATGTACCCAGGCATTGCCGACAGGATGCAGAAGGAAATTGTAACCTTGGCACCAAGTACCATGAA
  AATTAAGATCATAGCTCCCCCAGAACGCAAGTATTCGGTTTGGATTGGGGGCTCCATCCTCGCCAGCCTG
  TCCACATTCCAGCAGATGTGGATCAGTAAGCAGGAATATGACGAAGCTGGACCTCCCATTGTTCATAGAA
  AATGCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219887 representing NM_175497
  Red=Cloning site Green=Tags(s)
  
  MVDDELTALVVDNGSGMCKAGFGGDDAPRAVFPSMVGRPRHQGVMVGMGQKDCYVGDEAQSKRGILTLKY
  PIEHGVVTNWDDMEKIWYHTFYNELRVAPDEHPILLTEAPLNPKINREKMTQIMFEAFNTPAMYVAIQAV
  LSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYNFTTTAEREIV
  RDVKEKLCYVALDFEQEMVTAAASSSLERSYELPDGQVITIGNERFRCPEAIFQPSFLGIESRGIHETTF
  NSIMKCDVDIRKDLFANTVLSGGSTMYPGIADRMQKEIVTLAPSTMKIKIIAPPERKYSVWIGGSILASL
  STFQQMWISKQEYDEAGPPIVHRKCF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175497
• ORF Size: 1128 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175497.3, NP_780706.1
• RefSeq Size: 2737 bp
• RefSeq ORF: 1131 bp
• Locus ID: 238880
• UniProt ID: Q8BFZ3
• Cytogenetics: 13 D2.2
• Gene Summary: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.[UniProtKB/Swiss-Prot Function]