Anks1b (NM_001128086) Mouse Tagged ORF Clone

CAT#: MG219866

Anks1b (tGFP-tagged) - Mouse ankyrin repeat and sterile alpha motif domain containing 1B (Anks1b) transcript variant 1, (10ug)

Product Images

Circular map for MG219866

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Anks1b
• Synonyms: AIDA-1b; C030032C09Rik; E530015N03Rik; ENSMUSG00000078431; Gm1555; Gm10937
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219866 representing NM_001128086
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAGGACCAGGAGCTGCTGGAAGCTGCTCGTACTGGCAATGTGGCTCTGGTGGAGAAACTCCTAT
  CTGGCAGGAAAGGAGGGATCCTGGGTGGTGGATCCGGACCTCTGCCCCTGTCTAATCTGCTAAGCATCTG
  GCGAGGCCCCAATGTGAATTGCACAGACAGTTCGGGTTACACCGCTTTACACCACGCAGCCTTAAATGGA
  CATAAGGACATAGTTCTCAAACTGCTGCAGTACGAGGCATCCACCAATGTAGCAGACAACAAGGGTTACT
  TCCCCATTCACCTGGCTGCCTGGAAAGGAGACGTGGAGATTGTGAAGATCCTCATCCATCACGGACCTTC
  CCATTCCCGAGTCAATGAACAGAACAACGAGAACGAAACCGCCCTCCACTGTGCAGCGCAATACGGACAC
  TCGGAAGTAGTTGCGGTTCTCCTAGAGGAACTCACGGACCCCACGATTAGGAACAGCAAACTCGAAACAC
  CGCTGGACTTGGCAGCACTGTACGGGCGGCTGAGGGTGGTGAAGATGATCATCAGCGCACACCCAAACTT
  AATGAGCTGTAACACCCGGAAGCACACGCCGCTCCACCTCGCAGCCCGCAACGGTCACAAGGCCGTGGTC
  CAGGTGCTGCTGGAGGCGGGGATGGACGTGAGCTGTCAAACAGAAAAGGGGAGTGCTCTTCACGAAGCGG
  CTTTGTTTGGGAAGGTGGATGTGGTTCGAGTTCTGTTAGAAACAGGAATTGATGCCAACATAAAGGACAG
  TTTAGGTAGAACTGTCTTAGACATTCTAAAAGAGCACCCGTCTCAGAAGTCTCTCCAGATTGCAACGCTC
  TTGCAAGATTACTTGGAAGGCGCAGGAAGGTCAGCAGCAGTCCTGGAAGAGCATGCTCAGGAAGATACAG
  CACAAGAGACACACCTTTCGTCTCCTGCCGAGTCTCCTCAAAAGACTAAAAGTGAAACTGTCACTGGAGA
  ACTATCAAAACTCCTGGATGAAATAAAACTCTGCCAAGAAAAGGACTATTCCTTTGAAGATCTGTGCCAT
  ACGATCTCAGACCACTACTTAGATAACTTGAGCAAGATTTCAGAGGAAGAGCTTGGGAAAAATGGAAGCC
  AGAGTGTAAGAACTTCATCTACAATAAATTTGTCACCAGGAGAAGTGGAAGACGAAGAGGAGGATCCAAA
  CTCTTGTGGGCCCACAGGACTCTGGGAAGCGCTAACTCCTTGTAACGGATGTAGGAACCTTGGCTTTCCC
  ATGCTCGCACAGGAGTCTTATCCGAAGAAGAGGAATTTCCCCATGGAAATGGAACCATCTGCTTCTCTGG
  ACACATTCCCTTCGGAAAACGAGAACTTCCTTTGTGAACTCGTGGACACAGCTGTTACAAAGAAACCCTG
  CTCCTTAGAAATCGCAAGGGCACCTTCTCCGAGAACTGATAATGCCTCTGAGGTAGCAATTACTGCTCCA
  GGAACTAGTCACCATAGAAACAGCTCTACAGGCCCAACACCTGACTGCTCACCTCCATCCCCAGATACTG
  CCCTAAAAAATATCGTAAAAGTTATCCGACCTCAGCCAAAACAACGGACATCTATTGTGTCTTCTCTGGA
  TTTCCAGCGAATGAATCATAACCAAGAATACTTTGAAATCAGCACATCTACAGGGTGTACCAGCTTTACT
  TCCAGCCCTGCTGCTAGCCCTCCCACCTCTTCTGTGGAAACCACAGAAGTCAAGAACGAGGGAGCTGAAC
  ACGCAGATGATCTCTCTCAGCAGGAAGATGACGAACCCCCAAAAGAGTATGATGCTGGGCAATTCGCAGG
  CCTTCTCCATGGATCCTCTCCAGCCTGTGAGTCTCCTGAAAATCCGTTTCATCTGTATGGGAAAAGAAAT
  ACATGTGAAGATGGACCAGATGAAGCCAGTTTGGCAAACAGTCCTTTGCCTTTCAAGCAGACTCCAATAG
  AAAACAACCCGGAACCTTCGGTGAAGAAAGTTAAACCCAAAGTGGTCAGTAGAACAATTTTCCACAAAAG
  AAACCACCAACTTGAGAACCATACCATCGTTGGCACAAGAATGTCCAGGAGTGGATCCCGCAATGGTGAC
  CAATGGGGTGTGAATCCAGGGGGCTTTGTGGAGAGAGCATGTACTCTGGGGAGAATAAGGTCATTGCCTA
  AAGCCTTGATCGACATGCATTTATCCAAAAATGTCTCCAAGTCCGATTCTGATCTTATTGCCTACCCTTC
  CAAGGACAAAGCGAGAGTTAACTGGAGCAAATCGTCAACTGCGGAGCGCAGTTCCAAAGATAATTCTGAG
  AGAACACCTTCTTTCACATCTGAATGGGAAGAAATCGACAAAATAATGAATTCGATTGACGTTGGAATCA
  ACAGTGAACTTGAAGGGATGAATGGACCCAGATGCCCTGTCCAAACAGTGGGACAATGGCTGGAAAGCAT
  TGGGCTACCTCAGTACGAGAACCACCTGATGGCTAATGGATTTGACAGTGTACAGTTTATGGGAAGCAAT
  GTTATGGAAGATCAGGATTTGTTGGAAATTGGAATCCTTAACTCTGGCCACAGACAGAGAATTCTACAAG
  CGATCCAGCTCCTTCCAAAGATGAGGCCCATCGGCCATGATGGCTACCATCCCACCTCTGTAGCCGAGTG
  GCTGGATTCCATTGAGCTGGGTGATTACACCAAAGCCTTTCTCATCAATGGCTACACGTCCATGGACCTG
  TTGAAAAAGATCTGGGAGCTTGAGCTTATTAATGTTTTAAAAATCAGTTTGATTGGCCACAGGAAACGCA
  TTTTGGCATCTTTGGGAGATAGGCTGCACGACGACCCTCCACAGAAGCCCCCTCGGTCCATCACCCTCAG
  GGAACCCAGTGGTAATCACACTCCTCCTCAGTTGTCTCCATCACTTAGCCAAAGCACTTACACCACTGGT
  GGCTCCCTAGACGTTCCTCACATTATCATGCAGGGCGATGCAAGGAGGAGAAGAAATGAAAACTACTTTG
  ATGATATTCCCCGATCAAAGCTGGAGAGGCAGATGGCTCAGACAGGGGACTGGGGAGAACCTTCCATCAC
  CTTGCGACCTCCTAATGAAGCCACAGCCTCAACTCCCGTCCAGTACTGGCAGCATCATCCGGAAAAACTC
  ATCTTCCAGTCATGTGACTACAAAGCCTTTTATTTAGGTTCTATGCTGATAAAAGAGCTGAGGGGGACAG
  AATCAACACAGGATGCTTGTGCAAAGATGCGGGCTAACTGTCAGAAGTCTACAGAGCAAATGAAGAAAGT
  CCCCACTATCATCCTTTCCGTCTCATATAAAGGAGTAAAATTTATCGATGCGGCAAATAAGAATATAATC
  GCCGAACATGAAATTCGTAATATCTCCTGTGCTGCCCAGGACCCAGAAGACCTCTCAACATTTGCCTATA
  TCACAAAAGATTTGAAGTCCAACCACCACTACTGTCACGTGTTTACTGCCTTTGATGTGAATTTAGCCTA
  TGAAATCATCCTGACGCTGGGACAGGCATTTGAGGTCGCTTACCAACTAGCCCTACAAGCAAGAAAAGGG
  GGCCATTCCTCCACACTCCCAGAAAGCTTTGAGAACAAGCCCTCGAAACCCATCCCAAAGCCCCGAGTTA
  GCATTCGGAAGTCAGTGCAGATCGACCCTTCTGAGCAAAAGACTCTGGCCAATCTGCCGTGGATTGTGGA
  GCCGGGGCAAGAAGCCAAGAGGGGCATTAATACCAAGTATGAAACCACGATTTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219866 representing NM_001128086
  Red=Cloning site Green=Tags(s)
  
  MGKDQELLEAARTGNVALVEKLLSGRKGGILGGGSGPLPLSNLLSIWRGPNVNCTDSSGYTALHHAALNG
  HKDIVLKLLQYEASTNVADNKGYFPIHLAAWKGDVEIVKILIHHGPSHSRVNEQNNENETALHCAAQYGH
  SEVVAVLLEELTDPTIRNSKLETPLDLAALYGRLRVVKMIISAHPNLMSCNTRKHTPLHLAARNGHKAVV
  QVLLEAGMDVSCQTEKGSALHEAALFGKVDVVRVLLETGIDANIKDSLGRTVLDILKEHPSQKSLQIATL
  LQDYLEGAGRSAAVLEEHAQEDTAQETHLSSPAESPQKTKSETVTGELSKLLDEIKLCQEKDYSFEDLCH
  TISDHYLDNLSKISEEELGKNGSQSVRTSSTINLSPGEVEDEEEDPNSCGPTGLWEALTPCNGCRNLGFP
  MLAQESYPKKRNFPMEMEPSASLDTFPSENENFLCELVDTAVTKKPCSLEIARAPSPRTDNASEVAITAP
  GTSHHRNSSTGPTPDCSPPSPDTALKNIVKVIRPQPKQRTSIVSSLDFQRMNHNQEYFEISTSTGCTSFT
  SSPAASPPTSSVETTEVKNEGAEHADDLSQQEDDEPPKEYDAGQFAGLLHGSSPACESPENPFHLYGKRN
  TCEDGPDEASLANSPLPFKQTPIENNPEPSVKKVKPKVVSRTIFHKRNHQLENHTIVGTRMSRSGSRNGD
  QWGVNPGGFVERACTLGRIRSLPKALIDMHLSKNVSKSDSDLIAYPSKDKARVNWSKSSTAERSSKDNSE
  RTPSFTSEWEEIDKIMNSIDVGINSELEGMNGPRCPVQTVGQWLESIGLPQYENHLMANGFDSVQFMGSN
  VMEDQDLLEIGILNSGHRQRILQAIQLLPKMRPIGHDGYHPTSVAEWLDSIELGDYTKAFLINGYTSMDL
  LKKIWELELINVLKISLIGHRKRILASLGDRLHDDPPQKPPRSITLREPSGNHTPPQLSPSLSQSTYTTG
  GSLDVPHIIMQGDARRRRNENYFDDIPRSKLERQMAQTGDWGEPSITLRPPNEATASTPVQYWQHHPEKL
  IFQSCDYKAFYLGSMLIKELRGTESTQDACAKMRANCQKSTEQMKKVPTIILSVSYKGVKFIDAANKNII
  AEHEIRNISCAAQDPEDLSTFAYITKDLKSNHHYCHVFTAFDVNLAYEIILTLGQAFEVAYQLALQARKG
  GHSSTLPESFENKPSKPIPKPRVSIRKSVQIDPSEQKTLANLPWIVEPGQEAKRGINTKYETTIF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001128086
• ORF Size: 3765 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128086.3
• RefSeq Size: 7002 bp
• RefSeq ORF: 3768 bp
• Locus ID: 77531
• Cytogenetics: 10 C2
• Gene Summary: Isoform 2 may participate in the regulation of nucleoplasmic coilin protein interactions in neuronal and transformed cells.[UniProtKB/Swiss-Prot Function]