Unc93a (NM_199252) Mouse Tagged ORF Clone

CAT#: MG219586

Unc93a (tGFP-tagged) - Mouse unc-93 homolog A (C. elegans) (Unc93a), (10ug)

Product Images

Circular map for MG219586

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Unc93a
• Synonyms: unc-93A; Unc93l
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219586 representing NM_199252
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGGAGTCTGAAGAATGTTCTGGTGGTTTCTTGTGGCTTCCTCCTGCTCTTCACTGCCTACGGGG
  GCCTGCAGAATCTGCAGAGCAGCCTCTACAGTGAGCAGGGCCTGGGGGTGGCGACACTCAGCACACTCTA
  TGCCTCTGTGCTGCTGTCCTCCATGTTCCTCCCACCTATCCTCATCAAGAAGTGTGGCTGCAAGTGGACC
  ATCGTGGGCTCCATGTGCTGCTATGTGGTCTTCTCCCTGGGCAACTTCCATGCCAACTGGTACACCCTGA
  TCCCTACCTCCATCTTGCTGGGCCTGGGTGCGGCCCCTCTGTGGTCGGCTCAGGGTACCTACCTCACCAC
  CATGGGAAATCTGCAAGCGGAAAAGGTGGGGAAGCTTGGCAAAGATGTGGTGAACCAGTATTTTGGCATC
  TTTTTCCTGGTATTCCAATCTTCTGGAGTGTGGGGCAACCTGATTTCCTCACTGGTGTTCGGCAAGATGT
  CCATGCAAGAGGCCATCCCAGAGGAACAGCTCATGTCCTGTGGAGCCAAAGACTGCCTGATGGGTCCAGC
  GGCCACCAACAGTACTCATCACCCCTCACAGCAGCTGATCTACACACTGCTGGGCATCTACACCGGCTGT
  GGTGTCCTGGCCATCCTGCTGGTAGCTGTGTTTCTGGAGTCCTTGGAAGATAAGCTTGAGAATGAAGGAG
  AGAGGAGGCCCAGACCACCACCTCTTTGGTCCACATTACTGTCAACCTTCATGCTGTTTAGAGACAAGCG
  TTTGTGTCTCCTGATGTTCCTGCCACTGTACAGTGGGTTTCAACAAGAGTTCCTCTCTGGAGAGTACACA
  AAGTCTTATGTGACCTGTGCCCTGGGCATCCACTTTGTGGGCTACGTGATGATCTGCTTCTCAGCCATGA
  CTGCACTGTGCTCCCTGCTGTATGGGAAGATCTCCAAGTACACGGGCAGGGCAGCGCTCTATGTGCTGGG
  AGCAGCCATTCACTTCTCTTGCATTGTGGTCTTCCTGCTGTGGCACCCAAACACCAACCAACTGCCTGTC
  TTCTTCGTCCTCTCTGGCCTGTGGGGAATGTCTGATGCTGTCTGGCAGACACAGAACAATGCTCTTTTCG
  GTGTCCTGTTTGAGGAGAACAAAGAACCTGCTTTTGCCAACTACCGCCTTGGAGAAGCCATAGGGTTTGT
  CATTGCCTTTGGCTACAGCTCATTTCTGTGTGTGAGTACCAAGCTCTACATCCTCTTGGGTGTCTTGAGT
  CTGGCCATGGTTGGTTACGGGACAGTTGAGTACCTGGAAGTCAAGGCTGCTTCCAAGGTCCTTGGTGCAG
  AAAAGAAAAACCAGGCAGAAGAGGAAGAAATGAAAACAAAAATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219586 representing NM_199252
  Red=Cloning site Green=Tags(s)
  
  MERSLKNVLVVSCGFLLLFTAYGGLQNLQSSLYSEQGLGVATLSTLYASVLLSSMFLPPILIKKCGCKWT
  IVGSMCCYVVFSLGNFHANWYTLIPTSILLGLGAAPLWSAQGTYLTTMGNLQAEKVGKLGKDVVNQYFGI
  FFLVFQSSGVWGNLISSLVFGKMSMQEAIPEEQLMSCGAKDCLMGPAATNSTHHPSQQLIYTLLGIYTGC
  GVLAILLVAVFLESLEDKLENEGERRPRPPPLWSTLLSTFMLFRDKRLCLLMFLPLYSGFQQEFLSGEYT
  KSYVTCALGIHFVGYVMICFSAMTALCSLLYGKISKYTGRAALYVLGAAIHFSCIVVFLLWHPNTNQLPV
  FFVLSGLWGMSDAVWQTQNNALFGVLFEENKEPAFANYRLGEAIGFVIAFGYSSFLCVSTKLYILLGVLS
  LAMVGYGTVEYLEVKAASKVLGAEKKNQAEEEEMKTKI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199252
• ORF Size: 1374 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199252.2, NP_954860.1
• RefSeq Size: 2666 bp
• RefSeq ORF: 1377 bp
• Locus ID: 381058
• UniProt ID: Q710D3
• Cytogenetics: 17 8.78 cM