Pik3c2g (NM_207683) Mouse Tagged ORF Clone

CAT#: MG219435

Pik3c2g (tGFP-tagged) - Mouse phosphatidylinositol 3-kinase C2 domain containing gamma polypeptide (Pik3c2g) transcript variant 1, (10ug)

Product Images

Circular map for MG219435

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pik3c2g
• Synonyms: C80387; PI3K-C2-gamma
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219435 representing NM_207683
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATACAGTTGGCAAACAGAGCCAAATCGCACTGAACCACAGGAAGATGGATCTGATACCCAACAAT
  TTCACCATACTAACCAGCACCTTTCTTCCAGGCAAGTCAGGTTGGGTTTTGATCAGCTGGTGGAAGAGAT
  CAATAATAAGACTCCACTGTCTGAGAGTGAAAAGGAAGAAGACACGTATTTTGTGCCAGATGCACCAAAC
  TTGGTTTCAAAATGGCCATCCATATATGAAACACACCCAAGACACTTCAGTGAATTCACTTCTCAGAGCC
  CTGACTCCTCCCAGCTTCGGTTTGGAAAACTATCAGCCATTGGTTTTAATCCTGCTGTACTACCAACTCA
  TCATCTCGTTCATGAGGGAGCCTCCTGGAGAAATCCCAGTGGAAAATATCATGGCATCGAGTATCCCAGA
  TTCGATGCTCTACCTCCATCATCCACTGGCCAGGGTGAGTGTAATCCACAAGGACAATCAGGAACCAAAC
  ATCATAACTACTGTGTAGAACATGAAGGCAATCTCCCCCATCATCATTCATCCTACTCAATGGACTCCAT
  ACCCAATAGAGAGAAAAGAAGGAGTGGAGATGTGGACCTTGTGGAACCTTCTCTGGAGTTCTCTAAAGAC
  TCCTTTCTACCCAGGACATCCGAGAACGTGTCAGTGGAAAGCACAGAGCCGATTGGTTGTCCCATTGAAA
  TAGTTGAGGTACCCCAAGGGAGTAACAAGAACCTGGCCTCCTTTTGTAACAAAGTAAAAAAGATAAGAGA
  ATCATACCATGCAAGTGACATTAATTCCAATTCTGGGAAGATCTGGGCCATCACCACAGCCTATCCATCT
  CGGCTCTTCGCTGACACCAAATTCAGAGTAAAAATTTCTATTGATAACTCAGCCCAACTCCTCCTTCTTA
  TGCCACACGCTAATTATCTTGTCAAAGACCTAATTGCTGAAATTCTACTTTTATGTGCAAATGAGCCGCT
  TTCCCCCAAAGAGTATCTTCTAAGTGTATGCGGTTCTGAGGAATTTTTACAGATGGATCACTCTCTAGGG
  GGCCACAAAATATTCCAGAAAAATAAATCTGTCATTCAACTTCATCTTCAGAAAAACAGGGACACTCCAG
  GAAAATTATCCCGGAAGAGTGAAGATGACCACAGTCCGTTTCATCTGAACCAACTTCTAGAATTTACACA
  TATCTGGAAAATATCCAGACAATGCCTCTCCACAGTAATGAAAAAATACAACCTCCACGTGGAACACCTG
  TTGAAACCCCAGAAAGACATGGAGGAGAAACATTTGTCATCCATGTTTTCTGGTAACCAACACACTTCTC
  AGCCACATGTGAATAATGTTCTTGAAGAAGTTAAAAACATATGCAGTGTTTTGGGGTGTATTGAAACCAA
  ACAAGTTTCAGATGCCGTGAAAGAACTAAATCTAATTCTGCAGAGACCATCACAGAATTTTCCTCAGAAT
  TCAGAGACTTCAAAAAAAGGCTTCATAGAGAGAGTGACAGCTGAACTATCAAGGTCCATCTATCAGCTAA
  TCGACGTGTACTGCAGTACCTTCTGTACAGATTTCCAGCCTGTGCACACACCCGGAGGTGTCTCCCGTGT
  CCACGCTGGGCTCCAGTCCCACCTGAGCTTCACGGTGTGTTCCCTGCACAATGTCCCAGAGACTTGGGCA
  CACAGCTACAAAGCATTTTCGTTTTCCTGCTGGCTCACATATGCTGGGAAGAAGCTGTGCCAAGTGAAAA
  GCTGCAGACCCCTGCCAGTCACAAAGTCATTCTCCCTTTTGGTCAACTGGAATGAAATAATCAATTTTCC
  TCTTGAGATAAAGTCACTTCCAAGAGAATCCATGCTCGTTATAAAACTGTTCGGGATTGACTCTGCCACC
  CACAGCACAAATCTGCTGGCCTGGACCTGCCTTCCACTGTTTCCGAGACAGGAGTCTGTGCTGGGGTCTA
  GGCTGTTCAGTGTGACGTTACAGAGTGAGCCTCCTATCGAAATGATAGCTCCAGGCGTATGGGATGGGAG
  CCAGCCTTCCCCACTGACCCTGCAGATAGATTTTCCAGACGCCGGGTGGGAGTATCTGAAACCTGAGTCT
  GAAGAGAACAGAACTGACCATGAAGAGCCGCCAAGAGAGTGTTTAAAACACATCGCCAAACTCTCCCAGA
  AGAAGTCTCCCTTGCTACTTTCTGAGGAAAAGAGGAGATATTTGTGGTTTTATCGTCTCTACTGCAACAA
  TGAGAACTCCTCCCTCCCTTTGGTCTTGGGCAGCGCCCCTGGTTGGGATGAAGAGACAGTTTCTGAAATG
  CATGCAATCTTGAGAAGGTGGACGTTTTCACACCCATGGGAAGCTCTTGGTCTTTTGACTTCCAGGTTTC
  CAGACCAAGACATTCGTGAAGTTGCCGTTCAACAATTAGACACCCTCTTGACTGACGAGCTGCTGGACTG
  CCTCCCACAGCTAGTGCAGGCTGTCAAGTTTGAGTGGAACCTCGAAAGTCCCTTGGTGGAACTCCTGCTT
  CGTCGCTCCTTGCAAAGCATCCGAGTGGCTCACCGTCTGTACTGGCTGCTACGGGATGCCCAAGGTGAAG
  CCTACTTTAAAAGCTGGTATCAGGAGCTTTTGGCCGCTCTCCAGTTCTGTGCAGGAGAAGCCCTGAATGA
  AGAGCTTTCCAAGGAGCAGAAACTTGTCAAACTTCTGGGTGATATTGGAGAAAAAGTGAAGTCAGCCAGT
  GACCCTCAGAGAAAGGATGTGCTAAAGAAAGAGATTGGCAGTCTAGAAGAATTCTTTAAAGATATAAAGA
  CTTGCCATCTTCCTCTGAACCCGGCACTGTGCATAAAAGGAATTGATCGGGATGCTTGCTCGTATTTCAC
  ATCCAACGCTTCGCCATTGAAGATTACTTTCATCAATGCTAATCCAATGGGCAAAAATATCAGTGTTATT
  TTTAAGGCCGGAGATGATCTTCGGCAGGATATGCTTGCTCTGCAGATTATTCAAGTGATGGACAACGCTT
  GGCTGCAGGAGGGCCTCGATATGCAAATGATCACTTATGGATGTCTATCCACAGGAAGAGCCCAAGGATT
  CATAGAGATGGTGCCTGATGCTGTAACACTGGCCAAGATCCATCTGCACTCTGGGCTGATCGGACCCCTG
  AAAGAAAACACCATCAAAAAGTGGTTCAGTCAGCACAACCACTTAAAGGAAGATTATGAAAAGGCCTTGA
  GGAACTTCTTTTACTCTTGTGCTGGCTGGTGTGTGGTGACATTCATCTTGGGAGTCTGTGACCGACATAA
  TGACAATATCATGCTGACAAAGTCAGGTCACATGTTTCATATTGACTTTGGAAAATTCTTGGGTCATGCG
  CAAACATTTGGCGGTATCAAAAGGGACAGAGCCCCTTTCATTTTTACTTCAGAGATGGAGTACTTTATTA
  CTGAGGGTGGAAAAAACACACAGCATTTTCAAGACTTCGTGGAGCTTTGCTGCAGAGCTTACAACATCGT
  CAGGAAACACAGCCAGTTGATCCTGAGTCTTCTAGAGATGATGCTGCATGCAGGACTGCCTGAGCTAAGG
  GGAATCGAAGACCTGAAGTATGTACATAACAATCTCCGCCCACAAGACACAGACCTGGAAGCCACAAGTC
  ATTTCACCAAGAAGATAAAGGAAAGTCTGGAGTGTTTCCCAGTGAAATTGAATAACCTGATCCACACACT
  TGCACAGATGCCAGCCTTAAGCCTTGCCAAACCTGCCCCTCAGACTCTTCTCCAGGAATCCTGCATCCTG
  AATAAGACCAGGACAATTCAGAGAGTCACAATTTTAGGGTTCAGCAAGACACACAGCAACCTGTACCTGA
  TGGAGGTGACATGCAGTGACAATAGGAGAAGCCTGACCAAGAAGTCCTTCGAGCAGTTTTACAGACTTCA
  CAGCCAAATGCAGAAGCAGTTCTCCTCCTTGGCTCTCCCAGAATTTCCTCACTGGTGGCATCTACCTTTC
  ACAGACTCAGATCACAAGAGAATCAGAGATCTGAGTCACTACGTGGAACAGGTGCTACGCGGATCTTATG
  AAGTTGCAAACAGTGATTGTGTACTTAGCTTTTTTCTCTCTGAACATATTCAACCAACACTTGAAGACTC
  TCCATTTGTGGACCCAGGTGAGAACTCTCTAGACAAGAGCCCTAAGGTGCAGTTACTGATGACGTATGAG
  GACTCGAGGCTCACCATCCTAGTGAAACACTTGAAAAACATCCATCTCCCAGATGGCTCTGTGCCCAGTG
  CACATGTTGAAATTTATCTTCTACCACATCCCAGTGAAGTTCGGAGAAAGAAAACAAAGTGTGTTCCAAA
  ATGCACTGACCCAACTTACAATGAAATTGTGGTATATGATGAAGTCTTGGGGCTCCAGGGCCATGTTTTA
  ATGCTTATCGTGAAGAGCAAAACTGTATTTGTGGGAGCAGTTAACATCCAGCTCTGCAGTGTACCCCTCA
  ATGAAGAAAAGTGGTACCCACTAGGAAACAGTATCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219435 representing NM_207683
  Red=Cloning site Green=Tags(s)
  
  MAYSWQTEPNRTEPQEDGSDTQQFHHTNQHLSSRQVRLGFDQLVEEINNKTPLSESEKEEDTYFVPDAPN
  LVSKWPSIYETHPRHFSEFTSQSPDSSQLRFGKLSAIGFNPAVLPTHHLVHEGASWRNPSGKYHGIEYPR
  FDALPPSSTGQGECNPQGQSGTKHHNYCVEHEGNLPHHHSSYSMDSIPNREKRRSGDVDLVEPSLEFSKD
  SFLPRTSENVSVESTEPIGCPIEIVEVPQGSNKNLASFCNKVKKIRESYHASDINSNSGKIWAITTAYPS
  RLFADTKFRVKISIDNSAQLLLLMPHANYLVKDLIAEILLLCANEPLSPKEYLLSVCGSEEFLQMDHSLG
  GHKIFQKNKSVIQLHLQKNRDTPGKLSRKSEDDHSPFHLNQLLEFTHIWKISRQCLSTVMKKYNLHVEHL
  LKPQKDMEEKHLSSMFSGNQHTSQPHVNNVLEEVKNICSVLGCIETKQVSDAVKELNLILQRPSQNFPQN
  SETSKKGFIERVTAELSRSIYQLIDVYCSTFCTDFQPVHTPGGVSRVHAGLQSHLSFTVCSLHNVPETWA
  HSYKAFSFSCWLTYAGKKLCQVKSCRPLPVTKSFSLLVNWNEIINFPLEIKSLPRESMLVIKLFGIDSAT
  HSTNLLAWTCLPLFPRQESVLGSRLFSVTLQSEPPIEMIAPGVWDGSQPSPLTLQIDFPDAGWEYLKPES
  EENRTDHEEPPRECLKHIAKLSQKKSPLLLSEEKRRYLWFYRLYCNNENSSLPLVLGSAPGWDEETVSEM
  HAILRRWTFSHPWEALGLLTSRFPDQDIREVAVQQLDTLLTDELLDCLPQLVQAVKFEWNLESPLVELLL
  RRSLQSIRVAHRLYWLLRDAQGEAYFKSWYQELLAALQFCAGEALNEELSKEQKLVKLLGDIGEKVKSAS
  DPQRKDVLKKEIGSLEEFFKDIKTCHLPLNPALCIKGIDRDACSYFTSNASPLKITFINANPMGKNISVI
  FKAGDDLRQDMLALQIIQVMDNAWLQEGLDMQMITYGCLSTGRAQGFIEMVPDAVTLAKIHLHSGLIGPL
  KENTIKKWFSQHNHLKEDYEKALRNFFYSCAGWCVVTFILGVCDRHNDNIMLTKSGHMFHIDFGKFLGHA
  QTFGGIKRDRAPFIFTSEMEYFITEGGKNTQHFQDFVELCCRAYNIVRKHSQLILSLLEMMLHAGLPELR
  GIEDLKYVHNNLRPQDTDLEATSHFTKKIKESLECFPVKLNNLIHTLAQMPALSLAKPAPQTLLQESCIL
  NKTRTIQRVTILGFSKTHSNLYLMEVTCSDNRRSLTKKSFEQFYRLHSQMQKQFSSLALPEFPHWWHLPF
  TDSDHKRIRDLSHYVEQVLRGSYEVANSDCVLSFFLSEHIQPTLEDSPFVDPGENSLDKSPKVQLLMTYE
  DSRLTILVKHLKNIHLPDGSVPSAHVEIYLLPHPSEVRRKKTKCVPKCTDPTYNEIVVYDEVLGLQGHVL
  MLIVKSKTVFVGAVNIQLCSVPLNEEKWYPLGNSII
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207683
• ORF Size: 4518 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207683.2, NP_997566.1
• RefSeq Size: 6358 bp
• RefSeq ORF: 4521 bp
• Locus ID: 18705
• UniProt ID: O70167
• Cytogenetics: 6 69.7 cM
• Gene Summary: Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. May play a role in SDF1A-stimulated chemotaxis.[UniProtKB/Swiss-Prot Function]