9930111J21Rik2 (NM_173434) Mouse Tagged ORF Clone

CAT#: MG219395

9930111J21Rik2 (tGFP-tagged) - Mouse RIKEN cDNA 9930111J21 gene 2 (9930111J21Rik2), (10ug)

Product Images

Circular map for MG219395

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: 9930111J21Rik2
• Synonyms: 9930111J21Rik; Irgb5; OTTMUSG00000005540
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219395 representing NM_173434
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCAGACTTCCTCTTCTACACCCCCTCCAAAAGAGGATCCTGATTTGACCTCCAGCTTTGGCACAA
  ATCTTCAGAATTTCAAGATGAAAACCAAAATCCTGTCTCAGGAATTAATTGCCTTCATTGAATCATCCCT
  AGAGGATGGAAACCTTCAGGAAACAGTTTCTGCAATCAGCAGTGCTCTGGGTGGCATAGAGAAAGCCCCA
  CTGAACATTGCAGTGATGGGGGAGACTGGGGCTGGAAAGTCCAGTCTCATCAATGCTCTCCAGGGAGTGG
  GGGATGATGAAGAAGGTGCAGCTGCTTCCACAGGGGTGGTACATACAACCACTGAGAGAACACCATACAC
  TTACACAAAGTTTCCCAGTGTGACACTGTGGGACCTGCCTGGCATTGGATCTACTGCCTTCCAACCACAT
  GATTATCTGAAGAAAATCGAGTTTGAGGAGTATGACTTCTTCATTATTGTCTCTTCCGGACGCTTTAAAC
  ATAATGACGCAGAACTTGCCAAAGCCATTGTGCAAATGAATAGGAGTTTCTATTTTGTTCGAACCCACAC
  AGATCTTGATTTAATGGTTGTAAAACGGAGTAATCCTAGGAGATTCAACAGAGAGAACACATTAAAGCAG
  ATTCGACATACCATTTCAAGTATGCTTAAGGAAGTTACCCACCAGGAGCCTCCAGTCTTCTTAGTCTCTA
  ACTTTGATGTGTCTGACTTTGACTTCCCAAAGCTGGAATCCACCCTTCTGAGCCAGCTCCCAGCCTACAA
  ACACCACATGTTCATGCTCACTTTGCCTATTGTTACAGATTCCACCATAGACCGGAAGAGGGATATGCTG
  AAACAAAAGGTCTGGAAGGAATCCACAATGCCAAGAGCATGGGCTACCATACCATCCCTGGGACTGACCC
  AAAAGGACATGGAGATGTTGCAACAGACTTTGAATGACTATAGATCTTCCTTTGGCCTGGATGAGGCATC
  ACTGGAAAACATCGCTGAGGATTTGAACGTGACACTGGAGGAGCTCAAGGCAAACATTAAGTCTCCACAT
  TTGCTCTCAGATGAGCCAGATACATCCTTAACGGAGAAACTATTGAAGTACATTGGAAATCCTTACTTTT
  CAAAGGTTTTCCATTTGCAAAATTATTTCATTGACACGGTTGCGAGCGATGTTAAAATTATCCTCAGTAA
  AGAAGAGCTTTTCACAGAGCAGGTGAGCTCATTCAACTCTAAGGCCTCTCTATATCGGGAGGAGTCCGTG
  GGCAAAGTGTTTCCAGTTGGTCCAGGAAGCACCTTTCTATTTCATTTCATTGAAATGTTTCAAAGTGACT
  CTGACGAACTTTGTCATGTCCATGTTCTGCTCCTGTTGACATCCGGGGGCCTCTCTAGTGAGACTGTTAC
  A
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219395 representing NM_173434
  Red=Cloning site Green=Tags(s)
  
  MGQTSSSTPPPKEDPDLTSSFGTNLQNFKMKTKILSQELIAFIESSLEDGNLQETVSAISSALGGIEKAP
  LNIAVMGETGAGKSSLINALQGVGDDEEGAAASTGVVHTTTERTPYTYTKFPSVTLWDLPGIGSTAFQPH
  DYLKKIEFEEYDFFIIVSSGRFKHNDAELAKAIVQMNRSFYFVRTHTDLDLMVVKRSNPRRFNRENTLKQ
  IRHTISSMLKEVTHQEPPVFLVSNFDVSDFDFPKLESTLLSQLPAYKHHMFMLTLPIVTDSTIDRKRDML
  KQKVWKESTMPRAWATIPSLGLTQKDMEMLQQTLNDYRSSFGLDEASLENIAEDLNVTLEELKANIKSPH
  LLSDEPDTSLTEKLLKYIGNPYFSKVFHLQNYFIDTVASDVKIILSKEELFTEQVSSFNSKASLYREESV
  GKVFPVGPGSTFLFHFIEMFQSDSDELCHVHVLLLLTSGGLSSETVT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173434
• ORF Size: 1401 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173434.1, NP_775610.1
• RefSeq Size: 2403 bp
• RefSeq ORF: 1404 bp
• Locus ID: 245240
• Cytogenetics: 11 B1.2