Parg (NM_011960) Mouse Tagged ORF Clone

CAT#: MG219292

Parg (tGFP-tagged) - Mouse poly (ADP-ribose) glycohydrolase (Parg), (10ug)

Product Images

Circular map for MG219292

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Parg
• Synonyms: AI413217
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219292 representing NM_011960
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGCGGGCCCCGGCTGGGAGCCCTGCACGAAGCGGCCCCGCTGGGGCGCCGCTGGAACTTCTGCGC
  CGACTGCCTCGGACTCCCGGAGCTTCCCTGGCAGGCAGAGGCGTGTTCTCGACCCCAAGGACGCTCCCGT
  CCAGTTCAGGGTCCCTCCGTCCTCGCCAGCCTGCGTCTCGGGGCGGGCGGGACCGCACAGAGGCAACGCC
  ACCTCGTTTGTTTTCAAACAAAAGACTATTACTACTTGGATGGATACTAAAGGACCCAAGACAGCTGAAT
  CAGAAAGTAAAGAAAACAACAATACAAGAATTGACTCCATGATGAGTTCTGTGCAGAAAGATAACTTTTA
  CCCACATAAGGTGGAAAAATTGGAAAATGTTCCTCAGCTAAATCTTGATAAATCACCCACAGAAAAGAGT
  TCACAGTATTTGAACCAACAGCAGACTGCGAGTGTGTGCAAGTGGCAGAATGAAGGGAAGCATGCAGAAC
  AGCTTTTGGCAAGTGAGCCTCCCGCGGGGACTCCGCTACCAAAGCAGCTTAGTAATGCTAACATTGGTCA
  GTCACCCCACACTGATGACCACAGTGACACAGATCATGAAGAAGACAGAGACAATCAGCAGTTTCTTACA
  CCTATAAAACTTGCAAATACAAAGCCAACAGTAGGAGATGGGCAGGCCAGAAGCAACTGTAAGTGCAGTG
  GATCTCGCCAGTCTGTGAAAGACTGTACAGGCTGTCAACAGGAGGAGGTGGATGTGCTACCAGAGAGTCC
  TTTGTCAGATGTTGGTGCCGAGGACATTGGAACTGGACCAAAAAATGACAACAAATTGACTGGACAAGAA
  AGCAGCCTAGGTGATTCGCCTCCATTTGAGAAAGAAAGTGAGCCTGAGTCACCAATGGATGTAGACAACT
  CGAAAAACAGTTGTCAAGATTCAGAAGCAGATGAAGAAACAAGTCCAGTCTTTGATGAGCAAGATGATCG
  TTCCTCCCAAACAGCAAATAAACTTTCAAGTTGCCAAGCAAGAGAAGCTGATGGCGATCTTAGGAAACGG
  TATTTGACTAAGGGAAGTGAAGTTAGATTGCATTTCCAATTTGAAGGAGAAAATAATGCTGGGACCAGTG
  ACTTAAATGCCAAGCCATCTGGAAACTCTTCTAGCCTTAATGTAGAGTGTAGAAGTTCCAAGCAGCATGG
  AAAAAGGGATTCTAAAATTACAGATCATTTCATGAGAATTTCCAAGTCAGAGGACAGAAGAAAAGAACAA
  TGTGAAGTCAGACATCAAAGAACAGAAAGGAAGATTCCAAAATACATCCCACCTAACCTCCCTCCAGAGA
  AGAAGTGGCTGGGAACTCCTATTGAGGAAATGAGAAAAATGCCTCGGTGTGGGATCCATTTGCCTTCCTT
  AAGACCATCTGCAAGTCACACAGTGACTGTTCGGGTAGACCTTCTGAGAGCAGGAGAGGTTCCGAAACCT
  TTTCCAACACATTACAAAGATTTGTGGGATAACAAACATGTGAAAATGCCTTGTTCGGAACAAAACTTGT
  ACCCTGTGGAAGATGAGAATGGTGAGCGAACTGCAGGGAGTAGGTGGGAGCTCATTCAGACTGCACTTCT
  CAACAAATTCACACGACCCCAGAACTTGAAGGATGCGATTCTGAAATACAATGTGGCATATTCTAAGAAA
  TGGGACTTTACAGCTTTGGTTGATTTCTGGGATAAGGTACTTGAAGAAGCAGAGGCCCAACATTTATATC
  AGTCCATTTTACCTGACATGGTGAAAATTGCACTCTGTCTGCCAAATATTTGCACCCAGCCAATACCACT
  CCTGAAACAGAAGATGAATCATTCTGTCACGATGTCACAGGAACAGATCGCCAGTCTTTTAGCTAATGCT
  TTCTTCTGCACATTTCCCCGACGGAATGCCAAGATGAAATCGGAGTATTCTAGTTACCCAGACATTAACT
  TCAATCGGTTGTTTGAAGGACGTTCATCAAGGAAACCAGAAAAACTGAAAACACTCTTCTGCTACTTTCG
  AAGAGTCACAGAGAAAAAACCTACAGGATTGGTGACATTTACAAGACAGAGTCTTGAAGATTTTCCAGAA
  TGGGAAAGGTGTGAAAAGCCTCTGACACGCTTACACGTCACTTACGAGGGTACCATAGAAGGCAACGGCC
  GAGGCATGCTACAGGTGGATTTTGCAAATCGTTTTGTTGGAGGTGGTGTGACTGGTGCGGGACTTGTACA
  AGAAGAAATCAGATTTTTAATCAATCCTGAATTGATTGTTTCACGGCTGTTCACTGAGGTGCTGGATCAC
  AATGAGTGTCTTATTATCACAGGTACTGAACAGTACAGTGAATACACAGGCTATGCTGAAACTTATCGTT
  GGGCCCGAAGCCATGAAGATGGGAGTGAAAAGGACGATTGGCAGCGGCGCTGCACGGAGATCGTTGCCAT
  TGACGCACTTCACTTCAGACGCTACCTCGATCAGTTTGTGCCTGAGAAAGTGAGACGTGAGCTTAACAAG
  GCTTACTGCGGATTCCTCCGTCCTGGAGTTCCTTCTGAAAATCTTTCTGCAGTGGCCACGGGAAACTGGG
  GCTGTGGTGCCTTTGGGGGTGACGCTAGATTAAAAGCCTTAATACAGATCCTGGCAGCTGCTGCGGCTGA
  ACGTGACGTGGTTTATTTCACCTTTGGGGACTCAGAGTTGATGAGAGACATTTACAGCATGCACACTTTC
  CTTACCGAGAGGAAGCTGGATGTTGGAAAAGTGTACAAGTTATTGCTTAGATACTACAATGAAGAATGCA
  GAAACTGTTCCACCCCTGGACCAGACATCAAGCTTTATCCATTCATATACCATGCTGTTGAGTCAAGTGC
  AGAGACCACTGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219292 representing NM_011960
  Red=Cloning site Green=Tags(s)
  
  MSAGPGWEPCTKRPRWGAAGTSAPTASDSRSFPGRQRRVLDPKDAPVQFRVPPSSPACVSGRAGPHRGNA
  TSFVFKQKTITTWMDTKGPKTAESESKENNNTRIDSMMSSVQKDNFYPHKVEKLENVPQLNLDKSPTEKS
  SQYLNQQQTASVCKWQNEGKHAEQLLASEPPAGTPLPKQLSNANIGQSPHTDDHSDTDHEEDRDNQQFLT
  PIKLANTKPTVGDGQARSNCKCSGSRQSVKDCTGCQQEEVDVLPESPLSDVGAEDIGTGPKNDNKLTGQE
  SSLGDSPPFEKESEPESPMDVDNSKNSCQDSEADEETSPVFDEQDDRSSQTANKLSSCQAREADGDLRKR
  YLTKGSEVRLHFQFEGENNAGTSDLNAKPSGNSSSLNVECRSSKQHGKRDSKITDHFMRISKSEDRRKEQ
  CEVRHQRTERKIPKYIPPNLPPEKKWLGTPIEEMRKMPRCGIHLPSLRPSASHTVTVRVDLLRAGEVPKP
  FPTHYKDLWDNKHVKMPCSEQNLYPVEDENGERTAGSRWELIQTALLNKFTRPQNLKDAILKYNVAYSKK
  WDFTALVDFWDKVLEEAEAQHLYQSILPDMVKIALCLPNICTQPIPLLKQKMNHSVTMSQEQIASLLANA
  FFCTFPRRNAKMKSEYSSYPDINFNRLFEGRSSRKPEKLKTLFCYFRRVTEKKPTGLVTFTRQSLEDFPE
  WERCEKPLTRLHVTYEGTIEGNGRGMLQVDFANRFVGGGVTGAGLVQEEIRFLINPELIVSRLFTEVLDH
  NECLIITGTEQYSEYTGYAETYRWARSHEDGSEKDDWQRRCTEIVAIDALHFRRYLDQFVPEKVRRELNK
  AYCGFLRPGVPSENLSAVATGNWGCGAFGGDARLKALIQILAAAAAERDVVYFTFGDSELMRDIYSMHTF
  LTERKLDVGKVYKLLLRYYNEECRNCSTPGPDIKLYPFIYHAVESSAETTD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011960
• ORF Size: 2883 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011960.3
• RefSeq Size: 4391 bp
• RefSeq ORF: 2886 bp
• Locus ID: 26430
• Cytogenetics: 14 B