Lman1 (NM_001172062) Mouse Tagged ORF Clone

CAT#: MG219263

Lman1 (tGFP-tagged) - Mouse lectin mannose-binding 1 (Lman1) transcript variant 2, (10ug)

Product Images

Circular map for MG219263

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lman1
• Synonyms: 2610020P13Rik; AI326273; AU043785; C730041J05; ERGIC53; F5F8D; gp58; MCFD1; MR60; P58
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219263 representing NM_001172062
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGTCCAGGCGGAGGGTTCCCCAGGCTGGAGCGCGGTCGTTCTTCTGCGCCCTGCTGCTGTCTT
  TCAGCCAATTCACCGGGAGCGACGGCACGGGAGGCGACGCTGCGGCTCCCGGCGCCGCGGGCACCCAGGC
  CGAGTTGCCACACCGCCGTTTCGAGTACAAATACAGCTTTAAGGGGCCGCATCTGGTGCAGAGCGATGGG
  ACCGTGCCCTTTTGGGCCCACGCGGGGAATGCTATTCCAAGTGCAGATCAGATTCGAATAGCGCCGTCAT
  TAAAAAGCCAAAGAGGTTCCGTGTGGACCAAAGCAAAAGCAGCCTTCGAGAACTGGGAAGTGGAAGTGAC
  ATTCCGAGTGACCGGAAGAGGTCGAATTGGAGCCGATGGCTTAGCAATTTGGTATACAGAAAATCAAGGC
  TTGGACGGCCCTGTGTTTGGATCAGCTGATACGTGGAATGGTGTTGGAATATTTTTTGATTCTTTTGACA
  ATGATGGAAAGAAAAATAATCCTGCTATAGTAGTTATAGGCAACAATGGACAAATCAATTATGACCATCA
  AAATGATGGTGCCACTCAAGCTTTAGCGAGTTGTCAGAGGGACTTTCGTAATAAACCCTATCCTGTCCGG
  GCAAAGATTACGTATTACCAGAAGACACTGACAGTCATGATCAATAATGGCTTTACACCAGATAAAAATG
  ATTATGAATTCTGTGCCAAAGTGGAAAACATGGTTATCCCCACACAAGGTCATTTTGGGATATCAGCGGC
  AACAGGGGGACTTGCAGATGACCACGACGTTCTTTCTTTCCTGACTTTCCAACTGACCGAGCCTGGAAAA
  GAGCCACCTACAGCAGAAAAAGACATTTCAGAAAAAGAAAAGGAAAAGTATCAAGAGGAGTTTGAGCACT
  TCCAGCAAGAATTAGACAAGAAGAAGGAGGAATTCCAGAAGGGCCACCCTGACCTCCAGGGGCAGCCAGC
  GGATGACATCTTCGAGAGCATAGGCGATCGGGAGCTGAGACAGGTGTTTGAAGGACAGAATCGTATTCAT
  CTGGAGATCAAGCAGCTGAACCGGCAGCTGGATATGATTCTTGATGAACAGAGGAGGTACGTCTCGTCCC
  TGACAGAGGAGATCTCCAGGAGAGGAGCAGGAACCCCGGGACAGCCTGGGCAGGTCTCTCAGCAGGAACT
  GGACACCGTTGTGAAGAGCCAGCAGGAGATTCTGAGACAAGTGAATGAGGTGAAGAACTCCATGAGTGAA
  ACTGTGCGGCTGGTGAGTGGCATCCAGCACCCGGGCTCAGCAGGCGTCTACGAGACAACTCAGCACTTCA
  TGGACATCAAGGAACACCTGCACGTGGTGAAGAGAGATATCGACAGCCTCGCACAGCGCAGCATGCCATC
  TAATGAAAAACCAAAATGCCCAGACCTACCACCATTCCCGTCGTGTCTATCCACGATCCACTTTGTGATA
  TTTGTAGTGGTGCAGACAGTGTTGTTCGTTGGCTATATCATGTACAGGACTCAGCAAGAAGCAGCTGCCA
  AAAAATTCTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219263 representing NM_001172062
  Red=Cloning site Green=Tags(s)
  
  MAVSRRRVPQAGARSFFCALLLSFSQFTGSDGTGGDAAAPGAAGTQAELPHRRFEYKYSFKGPHLVQSDG
  TVPFWAHAGNAIPSADQIRIAPSLKSQRGSVWTKAKAAFENWEVEVTFRVTGRGRIGADGLAIWYTENQG
  LDGPVFGSADTWNGVGIFFDSFDNDGKKNNPAIVVIGNNGQINYDHQNDGATQALASCQRDFRNKPYPVR
  AKITYYQKTLTVMINNGFTPDKNDYEFCAKVENMVIPTQGHFGISAATGGLADDHDVLSFLTFQLTEPGK
  EPPTAEKDISEKEKEKYQEEFEHFQQELDKKKEEFQKGHPDLQGQPADDIFESIGDRELRQVFEGQNRIH
  LEIKQLNRQLDMILDEQRRYVSSLTEEISRRGAGTPGQPGQVSQQELDTVVKSQQEILRQVNEVKNSMSE
  TVRLVSGIQHPGSAGVYETTQHFMDIKEHLHVVKRDIDSLAQRSMPSNEKPKCPDLPPFPSCLSTIHFVI
  FVVVQTVLFVGYIMYRTQQEAAAKKFF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001172062
• ORF Size: 1551 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001172062.1, NP_001165533.1
• RefSeq Size: 1742 bp
• RefSeq ORF: 1554 bp
• Locus ID: 70361
• UniProt ID: Q9D0F3
• Cytogenetics: 18 E1
• Gene Summary: Mannose-specific lectin. May recognize sugar residues of glycoproteins, glycolipids, or glycosylphosphatidyl inositol anchors and may be involved in the sorting or recycling of proteins, lipids, or both. The LMAN1-MCFD2 complex forms a specific cargo receptor for the ER-to-Golgi transport of selected proteins (By similarity).[UniProtKB/Swiss-Prot Function]