Sytl2 (NM_031394) Mouse Tagged ORF Clone

CAT#: MG219061

Sytl2 (tGFP-tagged) - Mouse synaptotagmin-like 2 (Sytl2) transcript variant 3, (10ug)

Product Images

Circular map for MG219061

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sytl2
• Synonyms: AI266830; mKIAA1597; Slp2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219061 representing NM_031394
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCGACTTAAGTTTCCTGACAGAGGAGGAGCAAGACGCCATCTTAAAGGTTCTGCAGAGGGATGCTG
  CCCTGAAGAGGGCTGAAGAGGAGAGAGTCAGACATCTGCCTGAAAAAATTAAGGATGACCAACAACTAAA
  GAATATGAGTGGCCAATGGTTTTATGAAGCTAAGGCAAAAAGACACAGGGACAAAATCCACGGTGCTGAC
  ATCATCAGAGCATCCATGAGAAGGAAGAAGCTCCCAGCGGCAGCTGAGCAGAATAAGGACACAGCAATGA
  GGGCAAAGGAGAGCTGGGTGAATAATGTTAACAAAGATGCTGTCCTTCCTCCAGAGATAGCTGTTGTGGA
  AGAGCCAGAAGATGACACAGATCCCGCAGGCCCAAGTTCCAGTTTGGTGGATCCAGCTTCCAGTGTGATT
  GATATGTCCCAGGAAAGTACGAGGACTCCAGCTGTGTCTCTGCCCAAGCAAAGGAAGAATCCATTCAACA
  GCCCCAAGCTGCCGGAAGATCACTCCTTGCAACAAACCAAACCCGAGCAGTCAAAAACTGGAAAAGCTGG
  TTTATTTCAGATTTCAAAAGAGGGTGAGTTGTCAGAATCAAAAGAAAAGTCATCTATCCCAGATATGCCA
  CGTCAGCAGTTAGAGAAACCAAAGCAAACTGTATCCACAGAGCCTGAAAATGCGTCTCATACCAAAGCTC
  CAATCCCCAAAGCCAGAAAACTGATCTACAAATCAAATGACTTAGAGAAAGATGATAACCAGTCTTTTCC
  CAGACAACGGAGGGACTCCCTGAATGCGAGAGGGGCTCCAAGAGGGATCCTAAAGCGCAACTCCAGTTCC
  AGCAGCACGGACTCAGAGACCCTCCGTTTAAATTACAACCTTGATCCGAAAAGCAAAATCCTATCACCTG
  GCCTAACCATCCACGAGAGAATCTCTGAGAAGGAATTTTCTTTAGAAGACGACTCTTCCACAAGCTCACT
  AGAGCCGTTAAAGCACGTGAGATTCTCTGCCGTGAAGAATGAGCTTCCACAAAGTCCTAGACCCGTCCTT
  GGCCAGGAAGTGGGAGAATTTACTGTTTTAGAATCTGACCAGTTGCAAAATGGAACTGAAGATGCAGGGG
  ACATAGAGGAGTTTCAGAATCACCCAGAGCTCTCCCACAAAACACCTTTGTCTCATTATCAGTTAGTGTC
  AAGCCCAAGCGATTCAGGAAGGGAAAGAGAACAGCTGATGTCTTCTGGGTCTGCTCCAAGAGATGAGATC
  CCTTGTCATTCAGACATTCTACCCACAGGACCTCAGTGTGTTGAGAGTTCATCCGTCATCAATGGGCAAC
  AAGAGAAATCATCACATTTTACAAAGCTTCCATCAGAATTGTCCAAAAGCCCTTCCGATGAACTGACTCA
  GTGTGGTGAGCCTGAGCCATCACAGACAGCAGACCACAGTTTTAGAGACCATCGGCAAGGTTCAGAAGAA
  GAGCACAGCCCTGTTTTGAAAACTTTGGAAAGAAGAGCTGCTAGGAAATTGCCTTCCAAAAGTCTAGAAG
  ACATTCCATCAGATTCATCAAATCAAGCAAAAGTAGATAATCTGCCTGAAGAATTAGTGCGTAGTGCTGA
  AGATGATCAAAAAGCCGACCAAGAACCAGATACAAATGAATGTATACCAGGAATTTCCACAGTGCCTTCA
  CTGCCTGATAATCAGTTTTCCCACCCTGACAAACTCAAAAGGATGAGCAAGTCCGTGCCAGCATTTCTTC
  AAGATGAGGCGAGTGGCAGTGTGATGAGCGTTTACAGTGGAGACTTTGGCAACCTAGAAGTGAAAGGAAG
  CGTGCAGTTTGCACTCGACTACGTGGAGTCCCTGAAAGAGCTGCATGTGTTTGTGGCCCAGTGTAAGGAT
  TTAGCAGCAGCAGATGTTAAGAAACAGCGCTCAGATCCGTATGTAAAGACCTATCTGCTACCAGACAAAG
  GCAAAATGGGCAAGAAGAAGACACTCGTAGTGAAGAAGACCTTGAATCCTGTATACAACGAGATATTGCG
  GTATAAAATTGAAAGGCAATTCTTAAAGACGCAGAAGTTGAACCTGTCCGTTTGGCATCGGGATACATTT
  AAGCGCAACAGCTTTCTGGGGGAGGTGGAGCTCGACCTGGAAACGTGGGATTGGGACAGCAAACAGAACA
  AACAGCTGAAGTGGTACCCACTGAAGAGGAAGACAGCACCAGTTGCCCTCGAGACAGAAAACAGAGGTGA
  AATGAAACTAGCTCTCCAGTATGTTCCGGAACCAAGCCCTGGCAAAAAGCTTCCTACAACTGGAGAAGTC
  CACATCTGGGTGAAGGAATGCCTTGACCTCCCACTGTTGAGGGGCAGCCACCTAAATTCTTTTGTTAAAT
  GTACCATCCTTCCAGATACCAGTAGAAAAAGTCGCCAGAAGACAAGAGCTGTAGGGAAAACCACCAACCC
  CGTCTTCAACCATACCATGGTGTATGATGGGTTCAGGCCTGAAGATCTGATGGAAGCCTGTGTAGAACTC
  ACAGTCTGGGACCATTATAAACTAACCAACCAGTTTCTGGGAGGTCTCCGGATCGGCTTTGGAACAGGAA
  AAAGCTACGGGACTGAAGTGGATTGGATGGATTCTACTTCTGAGGAAGTTGCTCTCTGGGAGAAGATGGT
  AAACTCTCCCAACACTTGGGTTGAAGCGACGCTGCCCCTCCGGATGCTTCTGATTGCCAAGCTTTCCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219061 representing NM_031394
  Red=Cloning site Green=Tags(s)
  
  MIDLSFLTEEEQDAILKVLQRDAALKRAEEERVRHLPEKIKDDQQLKNMSGQWFYEAKAKRHRDKIHGAD
  IIRASMRRKKLPAAAEQNKDTAMRAKESWVNNVNKDAVLPPEIAVVEEPEDDTDPAGPSSSLVDPASSVI
  DMSQESTRTPAVSLPKQRKNPFNSPKLPEDHSLQQTKPEQSKTGKAGLFQISKEGELSESKEKSSIPDMP
  RQQLEKPKQTVSTEPENASHTKAPIPKARKLIYKSNDLEKDDNQSFPRQRRDSLNARGAPRGILKRNSSS
  SSTDSETLRLNYNLDPKSKILSPGLTIHERISEKEFSLEDDSSTSSLEPLKHVRFSAVKNELPQSPRPVL
  GQEVGEFTVLESDQLQNGTEDAGDIEEFQNHPELSHKTPLSHYQLVSSPSDSGREREQLMSSGSAPRDEI
  PCHSDILPTGPQCVESSSVINGQQEKSSHFTKLPSELSKSPSDELTQCGEPEPSQTADHSFRDHRQGSEE
  EHSPVLKTLERRAARKLPSKSLEDIPSDSSNQAKVDNLPEELVRSAEDDQKADQEPDTNECIPGISTVPS
  LPDNQFSHPDKLKRMSKSVPAFLQDEASGSVMSVYSGDFGNLEVKGSVQFALDYVESLKELHVFVAQCKD
  LAAADVKKQRSDPYVKTYLLPDKGKMGKKKTLVVKKTLNPVYNEILRYKIERQFLKTQKLNLSVWHRDTF
  KRNSFLGEVELDLETWDWDSKQNKQLKWYPLKRKTAPVALETENRGEMKLALQYVPEPSPGKKLPTTGEV
  HIWVKECLDLPLLRGSHLNSFVKCTILPDTSRKSRQKTRAVGKTTNPVFNHTMVYDGFRPEDLMEACVEL
  TVWDHYKLTNQFLGGLRIGFGTGKSYGTEVDWMDSTSEEVALWEKMVNSPNTWVEATLPLRMLLIAKLSK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031394
• ORF Size: 2730 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031394.2, NP_113571.2
• RefSeq Size: 3738 bp
• RefSeq ORF: 2733 bp
• Locus ID: 83671
• UniProt ID: Q99N50
• Cytogenetics: 7 E1
• Gene Summary: Isoform 11 acts as a RAB27A effector protein and plays a role in cytotoxic granule exocytosis in lymphocytes. Required for cytotoxic granule docking at the immunologic synapse. Isoform 1 may play a role in melanosome transport and vesicle trafficking. It controls melanosome distribution in the cell periphery and regulates melanocyte morphology. Isoform 1 acts as a positive mediator of mucus secretion by the surface mucus cells of the stomach. Mediates basal mucus secretion by gastric surface cells by promoting the proper granule biognesis and docking of mucus granules with the apical plasma membrane.[UniProtKB/Swiss-Prot Function]