Cdc14b (NM_001122989) Mouse Tagged ORF Clone

CAT#: MG218991

Cdc14b (tGFP-tagged) - Mouse CDC14 cell division cycle 14 homolog B (S. cerevisiae) (Cdc14b) transcript variant 2, (10ug)

Product Images

Circular map for MG218991

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cdc14b
• Synonyms: 2810432N10Rik; A530086E13Rik; AA472821; Cdc14B1; CDC14B3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218991 representing NM_001122989
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACGGGAGGGCGCAGGGACCCCTCTGATGGCCGAGGTGATCCGAGATCGCCTTTGTTTTGCCATTC
  TCTACAGCAGACCAAAGAGTGCAACAAACGAACATTATTTCAGCATAGATAACGAGCTAGAGTATGAGAA
  CTTCTATGCGGATTTTGGACCACTCAATCTGGCCATGGTTTACAGATATTGTTGCAAGATAAATAAGAAG
  CTCAAGTCCATTACAATGCTGAGGAAGAAAATTATCCATTTTACTGGCACTGATCAGAGAAAGCAAGCGA
  ATGCCGCCTTCCTTGTCGGGTGTTATATGGTTATATATTTGGGGAGGACACCAGAAGACGCGTATAGAAC
  ATTAATCTTCGGAGATACAGCCTATATTCCTTTCAGAGATGCTGCCTATGGAAGCTGCAGTTTCTATATT
  ACACTTCTCGACTGTTTTCACGCAGTAAAGAAGGCAATGCAGTATGGCTTCTTTAATTTCAACTCATTTA
  ACCTTGACGAATATGAACACTATGAAAAAGCAGAAAATGGAGATTTCAACTGGATAATACCAGAGCGATT
  TCTTGCCTTCTGTGGGCCCCATTCAAGATCCAGGCTGGAAAGTGGTTACCACCAACATTCTCCGGAGACT
  TATATTCCATATTTTAAGAATCACAATGTAACTACCATTATTCGTCTGAATAAAAGGATGTATGATGCTA
  AGCGCTTTACGGATGCTGGATTCGATCACCATGATCTTTTCTTTCCGGATGGCAGCACCCCTGCTGAGTC
  AATTGTCCAAGAATTTCTGGATATTTGTGAAAATGTCAAGGGTGCCATTGCTGTGCATTGCAAAGCTGGC
  CTTGGTCGAACAGGCACTCTGATAGGCTGCTACCTCATGAAGCATTACAGGATGACAGCAGCTGAGAGCA
  TTGCCTGGCTCAGAATCTGTAGACCCGGCTCAGTAATTGGGCCCCAGCAACAGTTTCTGGTCATGAAACA
  ATCAAGCCTCTGGCTAGAAGGCGACTATTTCCGTCAAAAGTTAAGAGGGCAGGAGAATGGCCCCCTCAGA
  GAAGCCTTCTCCAAACACCTTTCGGATGCTGATGACCTGTCCTTAAATGGGCTTGAGAATCAAGACAATC
  AAGAGCCTGAGCCGTATAGTGATGATGATGAAGTCAGTGGAATGACACAAGGAGACAGACTTCGGGCCCT
  GAAAAGCCGGAGACAGCCGAAAGCCAGCGCTATCCCCCTCACATGTCCCCTAGCTGTGCTGACCTCTGCA
  CTGTGTAGTGTTGCCATCTGGTGGATTGTGTGTGACTACATTCTTCCCACCCTGCTGTTCTGTCTTGATG
  GCTTCAGAACACAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218991 representing NM_001122989
  Red=Cloning site Green=Tags(s)
  
  MRREGAGTPLMAEVIRDRLCFAILYSRPKSATNEHYFSIDNELEYENFYADFGPLNLAMVYRYCCKINKK
  LKSITMLRKKIIHFTGTDQRKQANAAFLVGCYMVIYLGRTPEDAYRTLIFGDTAYIPFRDAAYGSCSFYI
  TLLDCFHAVKKAMQYGFFNFNSFNLDEYEHYEKAENGDFNWIIPERFLAFCGPHSRSRLESGYHQHSPET
  YIPYFKNHNVTTIIRLNKRMYDAKRFTDAGFDHHDLFFPDGSTPAESIVQEFLDICENVKGAIAVHCKAG
  LGRTGTLIGCYLMKHYRMTAAESIAWLRICRPGSVIGPQQQFLVMKQSSLWLEGDYFRQKLRGQENGPLR
  EAFSKHLSDADDLSLNGLENQDNQEPEPYSDDDEVSGMTQGDRLRALKSRRQPKASAIPLTCPLAVLTSA
  LCSVAIWWIVCDYILPTLLFCLDGFRTQ
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001122989
• ORF Size: 1344 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001122989.1, NP_001116461.1
• RefSeq Size: 5595 bp
• RefSeq ORF: 1347 bp
• Locus ID: 218294
• UniProt ID: Q6PFY9
• Cytogenetics: 13 B3
• Gene Summary: Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylates SIRT2 around early anaphase. Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases (By similarity).[UniProtKB/Swiss-Prot Function]