Rgl3 (NM_023622) Mouse Tagged ORF Clone

CAT#: MG218973

Rgl3 (tGFP-tagged) - Mouse ral guanine nucleotide dissociation stimulator-like 3 (Rgl3), (10ug)

Product Images

Circular map for MG218973

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rgl3
• Synonyms: 1300003D20Rik; mFLJ00153
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218973 representing NM_023622
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGGACAGCGGGCAAAGAGCTGGCCCTGGCGCCGCTGCAGGACTGGGGCGAGGAGACCGAGGATG
  GCGCCGTGTACAGCGTCTCCCTGCGGCGGCAGCGCAGCCAGCGCTCGACCCCAGAGAGGTCTGGAGAAGG
  CCAGACCCCCATCCCGGCTACCGACACCTTCCTTCATTACCGAACCAGCAAGGTGCGGGCGCTGAGGGCC
  GCCCGACTGGAGCGGCTGGTGCATGAGTTGGTGTCCGGAGACCGCGAGCAGGATCCTGGCTTCGTGCCTG
  CCTTCCTGGCCACCCACCGGGCCTTTGTACCCACAGCCCGCGTTTTGGGTTTTCTGCTACCCCCGCCACC
  GCCGCCGCCACCACCTCCTGCTGGGGTAGACAGCAAGAGGACAGAGGGACAAGATCTGAACTTCAGCAAG
  AACCTGAGGGCTGTAGTTTCGGTGCTGGGTTCTTGGCTGCGAAATCATCCTCAGGACTTCCGGGATCCCC
  CTGATCATCAGAACCTAGGCAATGTCCGAATCTTTTTGGGCTGGGCAGCCCCAGGAGGGGCAGAAGCCAG
  GGAAGCAGAGAAACTTCTAGAAGATTTCTTGAAGGAGGCTAAGGGAGAGCAGACAGAGGAGGAAAAGCGA
  CTGGCTTGGTCAGGCCCTCCTAGGATTGCCCAGACCCCCGGATCGGAGTTTGCGGAGGACTGTGTGGAAG
  AGGAAGGCCCCAGTTCAGAAGGCCCAGAGCTCCTGGACTTCAGCGTGGATGACGTGGCAGAGCAGCTGAC
  CCTTATGGATGTGGAGCTCTTCTTGCGCGTGAGGTCCTGCGAATGCCTGGGTTCCATGTGGTCTCAGCGC
  GACCGGCCAGGAGCTGCAGGCATCTCTCCCACAGTGCGTGCCACCGTAGCCCAGTTCAATACGGTGACCG
  GCTGCGTGCTGGGCTCGGTGCTCGCTGCGCCCGGCCTGGCGGCCTCGCAGAGGGCACAGCGCATCGAAAA
  ATGGATTCGCATCGCCCAGCGGTGCAGGGAACTACGAAATTTCTCTTCATTGCGAGCGATCCTGTCCGCC
  CTGCAATCTAACCCCATCTACAGACTCAAGCGCAGCTGGGGAGCTGTGAGCCGGGAACCGCTGTCTGTTT
  TCAGGAAACTGTCACAGATTTTTTCTGATGAGGATAACCATCTCAGCAGCCGAGCGATTCTTTCCCAGGA
  GGAGACCACTGAGGATGATGACTGCCCCTCAGGAAGTCTGCCATCAAAACTGCCCCCGGGTCCTGTCCCC
  TACCTTGGCACCTTCCTCACAGACTTGGTTATGCTGGATACAGCCTTACCGGATACGTTGAAGGGAAACC
  TCATCAACTTTGAAAAGAGGAGGAAGGAGTGGGAGATCCTGGCCCGAATCCAGCAGCTACAGCAACGCTG
  CCAGCGCTACAGCCTGAGCCCCCGCCCGCCAATCCTGGCTGCACTTCGAGCCCAGCGCCAGCTCAGCGAG
  GAGCAAAGCTACCGAGTCTCCCGTGTTATTGAGCCACCAGCTGCCTCCTGTCCCAGCTCCCCTCGTATCC
  GGCGACGCATCAGCCTCACAAAGCGTCTCAGTGCGAAGCTGTCCCGAGAGAAGAACTCATCTCCTGGCGG
  GAGTCCCGGGGATCCGTCCTCACCTACCTCCAGTGTGTCTCCAGGTTCACCCCCATCCAGCCCTAGAAAC
  CGAGAGCCTCCTCCTCCGGGCAGTCCTCCAGCCTCCCCAGGGCCTCAGAGCCCCAGCACCAAGCTCTCCC
  TGACCATGGACCCACCAGGGCCCTGGCCCGTGACCTTGACTCCGAGCAGCTCCCGAGTTCCCCTCCTCGG
  GCAGCAGACGTCAGAGGCCCGCGTCATCAGAGTCAGCATCAACAATAACCACGGGAACCTGTATCGGAGC
  ATCTTGCTTACTTGTCAGGACAAAGCCCCCAGCGTGGTGCAAAGAGCCCTGGAGAAGCACAATGTGCCCC
  AGCCCTGGGCCCGTGACTACCAGCTCTTCCAGGTCCTGCCTGGGGACAGAGAGCTCCTGATTCCAGACGG
  TGCCAACGTCTTCTACGCTATGAGCCCAGCTGCCCCTGGTGACTTCTTGCTTCGTCGGAAAGAGGGGACA
  GGACATACACTGTCTGCCTCCCCAACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218973 representing NM_023622
  Red=Cloning site Green=Tags(s)
  
  MERTAGKELALAPLQDWGEETEDGAVYSVSLRRQRSQRSTPERSGEGQTPIPATDTFLHYRTSKVRALRA
  ARLERLVHELVSGDREQDPGFVPAFLATHRAFVPTARVLGFLLPPPPPPPPPPAGVDSKRTEGQDLNFSK
  NLRAVVSVLGSWLRNHPQDFRDPPDHQNLGNVRIFLGWAAPGGAEAREAEKLLEDFLKEAKGEQTEEEKR
  LAWSGPPRIAQTPGSEFAEDCVEEEGPSSEGPELLDFSVDDVAEQLTLMDVELFLRVRSCECLGSMWSQR
  DRPGAAGISPTVRATVAQFNTVTGCVLGSVLAAPGLAASQRAQRIEKWIRIAQRCRELRNFSSLRAILSA
  LQSNPIYRLKRSWGAVSREPLSVFRKLSQIFSDEDNHLSSRAILSQEETTEDDDCPSGSLPSKLPPGPVP
  YLGTFLTDLVMLDTALPDTLKGNLINFEKRRKEWEILARIQQLQQRCQRYSLSPRPPILAALRAQRQLSE
  EQSYRVSRVIEPPAASCPSSPRIRRRISLTKRLSAKLSREKNSSPGGSPGDPSSPTSSVSPGSPPSSPRN
  REPPPPGSPPASPGPQSPSTKLSLTMDPPGPWPVTLTPSSSRVPLLGQQTSEARVIRVSINNNHGNLYRS
  ILLTCQDKAPSVVQRALEKHNVPQPWARDYQLFQVLPGDRELLIPDGANVFYAMSPAAPGDFLLRRKEGT
  GHTLSASPT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023622
• ORF Size: 2127 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023622.4, NP_076111.2
• RefSeq Size: 2550 bp
• RefSeq ORF: 2130 bp
• Locus ID: 71746
• UniProt ID: Q3UYI5
• Cytogenetics: 9 A3
• Gene Summary: Guanine nucleotide exchange factor (GEF) for Ral-A. Potential effector of GTPase HRas and Ras-related protein M-Ras. Negatively regulates Elk-1-dependent gene induction downstream of HRas and MEKK1.[UniProtKB/Swiss-Prot Function]