Rgl3 (NM_023622) Mouse Untagged Clone

CAT#: MC220830

Rgl3 (untagged) - Mouse ral guanine nucleotide dissociation stimulator-like 3 (Rgl3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rgl3
• Synonyms: 1300003D20Rik; mFLJ00153
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220830 representing NM_023622
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGGACAGCGGGCAAAGAGCTGGCCCTGGCGCCGCTGCAGGACTGGGGCGAGGAGACCGAGGATG
  GCGCCGTGTACAGCGTCTCCCTGCGGCGGCAGCGCAGCCAGCGCTCGACCCCAGAGAGGTCTGGAGAAGG
  CCAGACCCCCATCCCGGCTACCGACACCTTCCTTCATTACCGAACCAGCAAGGTGCGGGCGCTGAGGGCC
  GCCCGACTGGAGCGGCTGGTGCATGAGTTGGTGTCCGGAGACCGCGAGCAGGATCCTGGCTTCGTGCCTG
  CCTTCCTGGCCACCCACCGGGCCTTTGTACCCACAGCCCGCGTTTTGGGTTTTCTGCTACCCCCGCCACC
  GCCGCCGCCACCACCTCCTGCTGGGGTAGACAGCAAGAGGACAGAGGGACAAGATCTGAACTTCAGCAAG
  AACCTGAGGGCTGTAGTTTCGGTGCTGGGTTCTTGGCTGCGAAATCATCCTCAGGACTTCCGGGATCCCC
  CTGATCATCAGAACCTAGGCAATGTCCGAATCTTTTTGGGCTGGGCAGCCCCAGGAGGGGCAGAAGCCAG
  GGAAGCAGAGAAACTTCTAGAAGATTTCTTGAAGGAGGCTAAGGGAGAGCAGACAGAGGAGGAAAAGCGA
  CTGGCTTGGTCAGGCCCTCCTAGGATTGCCCAGACCCCCGGATCGGAGTTTGCGGAGGACTGTGTGGAAG
  AGGAAGGCCCCAGTTCAGAAGGCCCAGAGCTCCTGGACTTCAGCGTGGATGACGTGGCAGAGCAGCTGAC
  CCTTATGGATGTGGAGCTCTTCTTGCGCGTGAGGTCCTGCGAATGCCTGGGTTCCATGTGGTCTCAGCGC
  GACCGGCCAGGAGCTGCAGGCATCTCTCCCACAGTGCGTGCCACCGTAGCCCAGTTCAATACGGTGACCG
  GCTGCGTGCTGGGCTCGGTGCTCGCTGCGCCCGGCCTGGCGGCCTCGCAGAGGGCACAGCGCATCGAAAA
  ATGGATTCGCATCGCCCAGCGGTGCAGGGAACTACGAAATTTCTCTTCATTGCGAGCGATCCTGTCCGCC
  CTGCAATCTAACCCCATCTACAGACTCAAGCGCAGCTGGGGAGCTGTGAGCCGGGAACCGCTGTCTGTTT
  TCAGGAAACTGTCACAGATTTTTTCTGATGAGGATAACCATCTCAGCAGCCGAGCGATTCTTTCCCAGGA
  GGAGACCACTGAGGATGATGACTGCCCCTCAGGAAGTCTGCCATCAAAACTGCCCCCGGGTCCTGTCCCC
  TACCTTGGCACCTTCCTCACAGACTTGGTTATGCTGGATACAGCCTTACCGGATACGTTGAAGGGAAACC
  TCATCAACTTTGAAAAGAGGAGGAAGGAGTGGGAGATCCTGGCCCGAATCCAGCAGCTACAGCAACGCTG
  CCAGCGCTACAGCCTGAGCCCCCGCCCGCCAATCCTGGCTGCACTTCGAGCCCAGCGCCAGCTCAGCGAG
  GAGCAAAGCTACCGAGTCTCCCGTGTTATTGAGCCACCAGCTGCCTCCTGTCCCAGCTCCCCTCGTATCC
  GGCGACGCATCAGCCTCACAAAGCGTCTCAGTGCGAAGCTGTCCCGAGAGAAGAACTCATCTCCTGGCGG
  GAGTCCCGGGGATCCGTCCTCACCTACCTCCAGTGTGTCTCCAGGTTCACCCCCATCCAGCCCTAGAAAC
  CGAGAGCCTCCTCCTCCGGGCAGTCCTCCAGCCTCCCCAGGGCCTCAGAGCCCCAGCACCAAGCTCTCCC
  TGACCATGGACCCACCAGGGCCCTGGCCCGTGACCTTGACTCCGAGCAGCTCCCGAGTTCCCCTCCTCGG
  GCAGCAGACGTCAGAGGCCCGCGTCATCAGAGTCAGCATCAACAATAACCACGGGAACCTGTATCGGAGC
  ATCTTGCTTACTTGTCAGGACAAAGCCCCCAGCGTGGTGCAAAGAGCCCTGGAGAAGCACAATGTGCCCC
  AGCCCTGGGCCCGTGACTACCAGCTCTTCCAGGTCCTGCCTGGGGACAGAGAGCTCCTGATTCCAGACGG
  TGCCAACGTCTTCTACGCTATGAGCCCAGCTGCCCCTGGTGACTTCTTGCTTCGTCGGAAAGAGGGGACA
  GGACATACACTGTCTGCCTCCCCAACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023622
• Insert Size: 2130 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023622.4, NP_076111.2
• RefSeq Size: 2550 bp
• RefSeq ORF: 2130 bp
• Locus ID: 71746
• UniProt ID: Q3UYI5
• Cytogenetics: 9 A3
• Gene Summary: Guanine nucleotide exchange factor (GEF) for Ral-A. Potential effector of GTPase HRas and Ras-related protein M-Ras. Negatively regulates Elk-1-dependent gene induction downstream of HRas and MEKK1.[UniProtKB/Swiss-Prot Function]