Lvrn (NM_029008) Mouse Tagged ORF Clone

CAT#: MG218789

4833403I15Rik (tGFP-tagged) - Mouse RIKEN cDNA 4833403I15 gene (4833403I15Rik), (10ug)

Product Images

Circular map for MG218789

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Lvrn
• Synonyms: 4833403I15Rik; APQ; Aqpep
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218789 representing NM_029008
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCGTCCTTTCAGCTCCGGCGTCTATGTGAGCCGCGGGGTGGCCCTGCTGCTGGCCGCGCTGACAG
  CCGTGCTCCTGCTGGTGCTAGTGGCTCTGGCTTCCTTGTATGGCAGCTGCGCTCACGTCCAGCCTTCGGA
  GCAGGGTAACTCCCGGGTGAAGAACACCTCATTGTGGCCTCCCGGCGGCCAGGAGTGGGCGCTACCAACC
  CCCGCACAGGAGCCCACGGTGGGGACCTCCCAAGACTTGGGACCTCCGTCGGGGCCCTGGGATCATCTGC
  GCCTGCCTCCCTGGCTTGTCCCACTTCACTATGATCTGGAGCTGTGGCCCTGGCTGCAGCCGGATAAGCT
  GTCCCCACCGAATTTGACCTTCACGGGTCGCGTGAACATCACGGTACGCTGCACAGTGGCCTCCTCGAGA
  TTGTTGCTGCATAGCTTCCTTCTGAATTACAAGCAAGTGGAAGTGTGGGGTCCTCTGGCCCAGGACACTA
  GGAACGCCACTGTAGGCCGCGTGCAGGTGGAGAAAGTGTGGTTTGCGCCGGACATGCAATTCGTGGTGCT
  GGATCTGGGCCAATCCTTGGAGCCAGGTAGCAGATATGAGCTGAGTTTCCACTTCTCTGGCCAGGTGCTT
  CAGGTTGGCTTAGAAGGGCTCTTTCTGAACCTCTACCATGATGAAGATGAGCTCAGAGCCCTGGTAGCGA
  CTCAAATGGAGCCAACATTTGCCAGGCATGTTTTCCCTTGTTTTGATGAGCCAGCTCTAAAGGCAACTTT
  TAATATTACAGTTATCCATCATCCTGGGTATGCGGCTCTTTCCAATATGCCCCAGCTGGGTCAGTCTGAA
  AGAATCGATGTGAATGGAAGCAGATGGACTGTCACTACCTTCCATACCACACCCCGCATGCCAACGTACC
  TTGTTGCCCTTGTTGTGTGTGACTTAGACCACATCAGCAGAACTGAGAGAGGCAAGGAGATACGTGTCTG
  GGCCCGGAAAGACGACATTGCAAGTGGATATTTAGACTTTGCTGCAAACATCACAGGCCCCATCTTCTCA
  TTTCTGGAGGATCTGTTTAATATCAGTTATCGGCTTCCAAAAACAGATATAGTTGCCTTGCCTATTTTTG
  CCAGCGGCGCCATGGAGAACTGGGGCCTGTTGATATTTGACGAATCGTCCCTGCTCCTGGAACCTGAGGA
  TGAGCTGACGGAGAAAAGAGCTATGATCTTGAGCATCATCGCCCATGAGGTTGGGCACCAGTGGTTTGGA
  AACCTGGTGACCATGAGCTGGTGGAACAATATCTGGCTCAATGAAGGCTTTGCATCTTATTTTGAACTTG
  AACTAACTAACTACTTCTATCCTAAAGTTCCCATGAATATGATCTTTTTTTTTACCGTTTTACATGGAAT
  TCTCGGAGAAGATCATGCCCTGGAGTCTAGAGCTGTGTCCACTGCAGTGGAAAATTTCACAGAAACAAGC
  GAAATAAACAGACTCTTTGACTTATATACTTACAAGAAGGGAGCATGCATGGCCTGGATGCTTGCTAGTT
  TCCTGAGCCCGCACTTGTTTATCAACGCACTTAAGTCTTATTTGGAGACATTTTCTTACTCAAATGCTGA
  GCAAGATGACCTGTGGAGACATATTCAAATGGCTATAGGTGAGCAGAATGTAACTCATTTGCCAGCAACA
  GTAAAAGGCATAATGGACAGCTGGACACACCGGGGTGGCTTTCCTATCATCACAGTAAATGTGTCTACTG
  GCGTCGTTAAACAAGAGCCGTTTTATCTTGACAAGGTTGAAAATCAGACCCTTCTAAGCCACAATAGTAC
  ATGGATTGTCCCTATTTTTTGGATGAAAAATGGAATCCCACAACCTTTAGTCTGGCTAGATAAAAGCAAC
  ATGATGTTCCCTGGGATGAAACTTTCAGATTCTGACAGTGACTGGGTGATTTTAAACTTCAATGTGACTG
  GATATTACAGAGTTAATTACGATGAGTTAGGCTGGAAGAAGTTAAGTGAGCAACTTGAAAAGGATCCTAA
  GGCCATTCCTGTCATTAACAGGCTGCAGCTGGTGAGCGACGCTTTCGCCTTGACTAAAAACAATTACATT
  GAGATCGAGACGGCACTTGATTTAACCAAATACATTGCCGAAGAAGATGAGATCCTGGTGTGGTTTGAAG
  TCTTACTGAACTTGATAAACAGGGAAGTTATTTATGATGTGAACAACTATGCTTTATATCCACTATTGAA
  GAAGTATTTACTAAAGAGATTTAACTCAATATGGAATACTTATTCAGCTATAATTCGAGAAAATGTGGCA
  TCATTACAAGACAATTATTTATCTCTATTTTCCCTGGAAAGGATTTTTGAAACTGCCTGTTGGTTGGGTC
  TTGAAGACTGTCTCCAGCTGTCCAGAGAACTCTTCAAAAAATGGACGGCTCATCCAGAGACAGCAATCCC
  TCCTCAGATTGAAAACGCGGTCCTCTGCAGTGCCGTTGCTCTGGGGAGTGATAAGGACTGGGACTTTTTG
  TTTAACCTTTACATCAATACGACCGAGGAAGAGGACGAGAGCCGCCGCCGCCGTCTTGTTCACGCAATGA
  GCTGCAGTAAAGACCCGTGGATACTTAACAGATTTATGGAGTATGCCATCACTGCAGTTTCCTCCAATTT
  TAGTGAAACAAATATAATAGTTGTTGTAGCAACATCTGAAGTTGGCCGGTACATTGCAAAGGACTTTCTA
  ATGGACAACTGGGTAGCTGTGACTGAAAGGTATGGAATGGATTGGCTGGATGCTCTAGTGCCTGTCTTAG
  GGAGGACCGTCAGTACAGATCAGCAGGTCACAGAGTTGCAGCACTTCTTCAGTACTGTGTTGGAAGAGCA
  CCAGAGGGTTGTGGTTCAAGCCAAATTACAGGCAATGAAGACGAAGAATCTGCAAAACAAGAAGAGAATT
  GCCAGGGTGGTTGAGTGGCTTCGGAAAAACACG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218789 representing NM_029008
  Red=Cloning site Green=Tags(s)
  
  MSRPFSSGVYVSRGVALLLAALTAVLLLVLVALASLYGSCAHVQPSEQGNSRVKNTSLWPPGGQEWALPT
  PAQEPTVGTSQDLGPPSGPWDHLRLPPWLVPLHYDLELWPWLQPDKLSPPNLTFTGRVNITVRCTVASSR
  LLLHSFLLNYKQVEVWGPLAQDTRNATVGRVQVEKVWFAPDMQFVVLDLGQSLEPGSRYELSFHFSGQVL
  QVGLEGLFLNLYHDEDELRALVATQMEPTFARHVFPCFDEPALKATFNITVIHHPGYAALSNMPQLGQSE
  RIDVNGSRWTVTTFHTTPRMPTYLVALVVCDLDHISRTERGKEIRVWARKDDIASGYLDFAANITGPIFS
  FLEDLFNISYRLPKTDIVALPIFASGAMENWGLLIFDESSLLLEPEDELTEKRAMILSIIAHEVGHQWFG
  NLVTMSWWNNIWLNEGFASYFELELTNYFYPKVPMNMIFFFTVLHGILGEDHALESRAVSTAVENFTETS
  EINRLFDLYTYKKGACMAWMLASFLSPHLFINALKSYLETFSYSNAEQDDLWRHIQMAIGEQNVTHLPAT
  VKGIMDSWTHRGGFPIITVNVSTGVVKQEPFYLDKVENQTLLSHNSTWIVPIFWMKNGIPQPLVWLDKSN
  MMFPGMKLSDSDSDWVILNFNVTGYYRVNYDELGWKKLSEQLEKDPKAIPVINRLQLVSDAFALTKNNYI
  EIETALDLTKYIAEEDEILVWFEVLLNLINREVIYDVNNYALYPLLKKYLLKRFNSIWNTYSAIIRENVA
  SLQDNYLSLFSLERIFETACWLGLEDCLQLSRELFKKWTAHPETAIPPQIENAVLCSAVALGSDKDWDFL
  FNLYINTTEEEDESRRRRLVHAMSCSKDPWILNRFMEYAITAVSSNFSETNIIVVVATSEVGRYIAKDFL
  MDNWVAVTERYGMDWLDALVPVLGRTVSTDQQVTELQHFFSTVLEEHQRVVVQAKLQAMKTKNLQNKKRI
  ARVVEWLRKNT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029008
• ORF Size: 2973 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029008.1, NP_083284.1
• RefSeq Size: 3219 bp
• RefSeq ORF: 2976 bp
• Locus ID: 74574
• Cytogenetics: 18 C