Avl9 (NM_030235) Mouse Tagged ORF Clone

CAT#: MG218711

Avl9 (tGFP-tagged) - Mouse AVL9 homolog (S. cerevisiase) (Avl9), (10ug)

Product Images

Circular map for MG218711

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Avl9
• Synonyms: 5830411G16Rik; D730049P16Rik; mKIAA0241
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218711 representing NM_030235
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGTCTGGGCGTGAGAGTGACGGCGCCCCCTGCGGGCCTGTCCTGCACATCGTGGTGGTCGGCT
  TTCACCACAAGAAGGGCTGCCAGGTTGAATTCTCTTACCCACCACTAATTCCAGGAGATGGACATGATAG
  CCACACTTTACCTGAAGAATGGAAGTACTTGCCTTTCCTTGCCTTACCAGATGGTGCACATAACTATCAG
  GAAGATACTGTGTTTTTTCACTTGCCACCCAGAAATGGAAATGGAGCCACAGTATATGGTATCTCTTGTT
  ATCGACAAATTGAAGCCAAGGCATTAAAAGTGAGGCAAGCCGACATCACCAGAGAGACTGTTCAGAAAAG
  TGTCTGTGTTTTAAGCAAGCTGCCTCTTTATGGCTTACTTCAAGCAAAACTCCAACTCATTACACATGCA
  TATTTTGAAGAAAAAGATTTTTCCCAAATTTCCATTCTAAAGGAGCTCTATGAACATATGAACAGTTCCT
  TGGGAGGGGCCTCATTAGAAGGATCGCAGGTGTATCTTGGTTTGTCTCCTCGAGATCTTGTCCTTCATTT
  TCGACACAAGGTCTTGATCCTGTTTAAGCTAATTCTTCTTGAAAAGAAGGTTCTGTTTTACATTTCTCCA
  GTGAATAGATTGGTGGGTGCACTAATGACTGTGTTATCCCTTTTTCCAGGCATGATTGAGCATGGCCTCA
  GTGACTGTTCTCAGTATAGACCTCGAAAGAGTATGTCTGAAGATGCTGGACCTCAGGAAAGTAATCCCTC
  TGCAGATGATTTTACTTCTGAGTCTACTTCTGACGTCTTAAATACCAGCTTGGAAACTGTCACAAGAGTC
  ATGGCAGTAAACCATGGCGAAGATGCTGTGCCCAAGACGGAGAAGCCTTACTTCCAAGTAGAAGGTAACA
  ACAACAAAGGACAGGAACCCAGTGATTCTGGCCGATATTTGGAACTTCCACCCCGTCCATCTCCAGAGTC
  TTCAGAAAGTGACTGGGAAACGTTGGATCCTAGTGTCTTAGAGGATGCCAGCTTGAAAGAAAGGGAGCAG
  ATGGGGTCAGACCAGACACACTTATTCCAAAAGGACTCTCTGCCCTCGGACAGTCCTCCAATTACTGTAC
  AGCCTCAAGCTAATAACAGGCAGGTGGTCCTGATACCAGGGCTCATTTCTGGTTTGGAAGAAGACCAATA
  TGGCATGCCTCTGGCCATCTTTACAAAGGGATATCTGTGTTTGCCTTACATGGCATTGCAGCAGCATCAT
  CTTCTCTCTGATGTCACTGTTCGGGGATTTGTGGCTGGAGCTACTAACATCCTTTTCCGACAACAGAAGC
  ACCTCAGTGATGCCATTGTGGAAGTGGAAGAAGCACTGATCCAGATCCACGATCCAGAACTTAGGAAGCT
  GCTTAACCCAACGACGGCTGACCTAAGGTTCGCAGACTACCTGGTGAGGCACGTGACTGAGAACCGGGAC
  GATGTCTTCCTGGATGGCACCGGCTGGGAGGGTGGTGACGAATGGATCCGGGCCCAGTTTGCAGTCTACA
  TCCACGCTCTCCTGGCTGCTACACTGCAGTTAGATAATGAAAAGATGTTATCAGACTATGGAACAACTTT
  TGTTGCCGCCTGGAAGAATACTCACAACTACAGGGTATGGAACAGCAATAAGCATCCGGCACTTTCAGAG
  ATAAATCCAAACCATCCTTTTCAAGGCCAGTACTCCGTGTCAGACATGAAACTCAGATTCTCACATTCTG
  TTCAGAATAGTGAACGTGGCAAAAAAATTGGCAGTGTCATGGTCACAACCAGCCGGAATGTGGTACAAAC
  AGGAAAAGCCGTTGGCCAATCAGTTGGAGGAGCTTTTTCCAGTGCAAAGACAGCTATGTCTTCATGGCTA
  TCTACTTTCACCACATCCACCCCTCAGAGTCTTCCTGAGCCACCCAATGGGAAGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218711 representing NM_030235
  Red=Cloning site Green=Tags(s)
  
  MEKSGRESDGAPCGPVLHIVVVGFHHKKGCQVEFSYPPLIPGDGHDSHTLPEEWKYLPFLALPDGAHNYQ
  EDTVFFHLPPRNGNGATVYGISCYRQIEAKALKVRQADITRETVQKSVCVLSKLPLYGLLQAKLQLITHA
  YFEEKDFSQISILKELYEHMNSSLGGASLEGSQVYLGLSPRDLVLHFRHKVLILFKLILLEKKVLFYISP
  VNRLVGALMTVLSLFPGMIEHGLSDCSQYRPRKSMSEDAGPQESNPSADDFTSESTSDVLNTSLETVTRV
  MAVNHGEDAVPKTEKPYFQVEGNNNKGQEPSDSGRYLELPPRPSPESSESDWETLDPSVLEDASLKEREQ
  MGSDQTHLFQKDSLPSDSPPITVQPQANNRQVVLIPGLISGLEEDQYGMPLAIFTKGYLCLPYMALQQHH
  LLSDVTVRGFVAGATNILFRQQKHLSDAIVEVEEALIQIHDPELRKLLNPTTADLRFADYLVRHVTENRD
  DVFLDGTGWEGGDEWIRAQFAVYIHALLAATLQLDNEKMLSDYGTTFVAAWKNTHNYRVWNSNKHPALSE
  INPNHPFQGQYSVSDMKLRFSHSVQNSERGKKIGSVMVTTSRNVVQTGKAVGQSVGGAFSSAKTAMSSWL
  STFTTSTPQSLPEPPNGKP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030235
• ORF Size: 1947 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030235.1, NP_084511.1
• RefSeq Size: 6691 bp
• RefSeq ORF: 1950 bp
• Locus ID: 78937
• UniProt ID: Q80U56
• Cytogenetics: 6 B3
• Gene Summary: Functions in cell migration.[UniProtKB/Swiss-Prot Function]