Pklr (NM_013631) Mouse Tagged ORF Clone

CAT#: MG218708

Pklr (tGFP-tagged) - Mouse pyruvate kinase liver and red blood cell (Pklr) nuclear gene encoding mitochondrial protein transcript variant 1, (10ug)

Product Images

Circular map for MG218708

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pklr
• Synonyms: Pk-1; Pk1; R-PK
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218708 representing NM_013631
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTCCAAGAGAACGAGCTACCCCAGCAGCTCTGGCCCTGGATCTTTAAGTCCCAAAAAGACTTAG
  CAAAGTCTGCTTTAAGTGGGGCTCCAGGAGGGCCAGCAGGATACCTGAGACGTGCCAGTGTGGCCCAGCT
  GACCCAGGAGCTGGGCACTGCCTTCTTCCAGCAGCAGCAACTGCCCGCAGCTATGGCGGACACCTTCCTG
  GAACACCTCTGCCTTCTGGATATCGACTCAGAGCCTGTGGCCGCTAGGAGCACCAGCATCATTGCCACCA
  TCGGGCCGGCGTCACGCTCTGTGGACCGCCTCAAGGAGATGATCAAGGCAGGGATGAACATTGCACGACT
  CAACTTCTCCCATGGCTCCCATGAGTACCATGCAGAGTCCATCGCCAACATTCGGGAGGCGGCTGAAAGC
  TTTGCAACCTCCCCACTCAGCTACAGACCCGTGGCCATCGCCCTGGACACCAAGGGTCCCGAGATACGCA
  CTGGAGTCCTGCAGGGGGGTCCAGAGTCGGAGGTGGAAATTGTGAAGGGTTCACAGGTGCTGGTGACTGT
  GGATCCGAAGTTCCGGACAAGGGGCGATGCAAAGACAGTGTGGGTGGACTACCACAATATCACCCAGGTC
  GTTGCAGTGGGGGGCCGCATCTACATTGACGACGGGCTCATCTCCTTAGTGGTGCGGAAAATTGGCCCAG
  AGGGACTGGTGACCGAAGTGGAACACGGTGGTTTCTTGGGCAACAGGAAGGGTGTGAACTTGCCAAATGC
  CGAGGTGGACCTGCCTGGGCTATCAGAGCAAGATCTTTTGGATCTGCGCTTCGGGGTGGAGCATAATGTG
  GACATCATCTTTGCCTCCTTTGTACGAAAAGCCAGTGATGTGGTGGCAGTCCGAGATGCCCTAGGGCCAG
  AAGGACGGGGCATCAAAATTATCAGCAAAATCGAGAACCATGAAGGCGTGAAGAAGTTTGATGAGATCCT
  AGAAGTGAGCGATGGCATCATGGTGGCTCGGGGTGACCTTGGCATTGAGATCCCAGCAGAGAAGGTTTTC
  TTGGCTCAGAAGATGATGATTGGACGCTGCAACCTGGCTGGCAAGCCTGTCGTTTGTGCCACACAGATGC
  TGGAGAGCATGATCACTAAGGCTCGACCAACTCGGGCGGAGACAAGCGATGTGGCCAATGCTGTGCTGGA
  TGGGGCTGACTGTATCATGCTGTCTGGAGAGACCGCCAAAGGCAGTTTCCCCGTGGAGGCTGTAAAGATG
  CAACATGCGATTGCCCGGGAGGCAGAGGCCGCTGTGTACCACCGCCAGTTGTTTGAGGAGCTACGCCGGG
  CAGCGCCGCTGAGCCGTGACCCAACCGAGGTCACTGCAATTGGAGCCGTGGAGGCTTCCTTCAAGTGCTG
  TGCCGCAGCCATCATTGTGCTGACAAAGACTGGCCGCTCAGCTCAGCTTCTGTCTCGCTACCGACCTCGG
  GCTGCTGTCATTGCTGTGACTCGTTCTGCCCAGGCTGCCCGACAGGTCCACCTGTCCCGAGGAGTCTTCC
  CCTTGCTCTACCGTGAGCCTCCAGAGGCTGTCTGGGCAGATGATGTGGACCGAAGGGTCCAATTTGGCAT
  CGAAAGTGGAAAGCTTCGTGGCTTCCTCCGAGTTGGTGATCTGGTGATTGTGGTGACAGGCTGGCGACCT
  GGCTCTGGCTATACCAACATTATGCGGGTGCTGACCATATCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218708 representing NM_013631
  Red=Cloning site Green=Tags(s)
  
  MSVQENELPQQLWPWIFKSQKDLAKSALSGAPGGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFL
  EHLCLLDIDSEPVAARSTSIIATIGPASRSVDRLKEMIKAGMNIARLNFSHGSHEYHAESIANIREAAES
  FATSPLSYRPVAIALDTKGPEIRTGVLQGGPESEVEIVKGSQVLVTVDPKFRTRGDAKTVWVDYHNITQV
  VAVGGRIYIDDGLISLVVRKIGPEGLVTEVEHGGFLGNRKGVNLPNAEVDLPGLSEQDLLDLRFGVEHNV
  DIIFASFVRKASDVVAVRDALGPEGRGIKIISKIENHEGVKKFDEILEVSDGIMVARGDLGIEIPAEKVF
  LAQKMMIGRCNLAGKPVVCATQMLESMITKARPTRAETSDVANAVLDGADCIMLSGETAKGSFPVEAVKM
  QHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEASFKCCAAAIIVLTKTGRSAQLLSRYRPR
  AAVIAVTRSAQAARQVHLSRGVFPLLYREPPEAVWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRP
  GSGYTNIMRVLTIS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013631
• ORF Size: 1722 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013631.2, NP_038659.2
• RefSeq Size: 2787 bp
• RefSeq ORF: 1725 bp
• Locus ID: 18770
• Cytogenetics: 3 39.01 cM