Gja8 (NM_008123) Mouse Tagged ORF Clone

CAT#: MG218273

Gja8 (tGFP-tagged) - Mouse gap junction protein alpha 8 (Gja8), (10ug)

Product Images

Circular map for MG218273

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gja8
• Synonyms: Aey5; Cnx50; Cx50; Lop10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218273 representing NM_008123
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGACTGGAGTTTCCTGGGAAACATCTTGGAAGAGGTGAATGAGCACTCCACTGTCATCGGCAGAG
  TCTGGCTCACAGTGCTCTTCATCTTCCGCATCCTCATCCTCGGGACAGCAGCGGAGTTTGTGTGGGGCGA
  TGAGCAATCTGATTTTGTATGCAACACCCAGCAGCCAGGCTGTGAGAATGTCTGCTACGATGAGGCCTTT
  CCCATCTCACACATCCGCCTCTGGGTGCTGCAGATCATCTTCGTCTCCACTCCATCGCTGATGTACGTGG
  GGCACGCGGTACACCACGTTCGCATGGAGGAGAAGCGAAAGGACCGTGAAGCTGAGGAGCTCTGTCAGCA
  GTCGCGCAGCAACGGGGGTGAGAGGGTACCAATCGCCCCAGACCAGGCCAGCATCCGGAAGAGCAGCAGC
  AGTAGCAAAGGCACCAAGAAGTTCCGGCTGGAGGGCACACTGCTAAGGACCTATGTCTGCCACATCATCT
  TCAAGACCCTCTTTGAGGTGGGCTTCATCGTGGGCCATTACTTCCTGTATGGTTTCCGCATCCTGCCCCT
  CTATCGCTGCAGCCGGTGGCCCTGCCCCAATGTGGTAGACTGCTTTGTATCCCGGCCTACTGAGAAGACC
  ATCTTCATCCTCTTCATGTTATCAGTCGCTTTTGTGTCACTCTTCCTCAACATCATGGAGATGAGCCACC
  TGGGCATGAAAGGAATCCGGTCTGCCTTCAAGAGGCCTGTAGAGCAACCACTGGGGGAGATTGCTGAGAA
  GTCCCTCCACTCCATTGCAGTTTCCTCCATCCAGAAAGCCAAGGGCTACCAGCTTCTAGAAGAAGAGAAG
  ATCGTATCACACTATTTCCCTTTGACAGAGGTTGGAATGGTGGAGACCAGCCCTCTTTCGGCCAAGCCTT
  TTAGTCAGTTTGAGGAGAAGATCGGCACAGGACCCCTGGCAGATATGTCACGGAGTTACCAAGAAACCCT
  GCCTTCTTATGCTCAGGTGGGGGTCCAGGAAGTGGAGCGGGAAGAGCCGCCTATAGAAGAGGCTGTGGAA
  CCGGAAGTGGGAGAGAAGAAGCAAGAAGCAGAGAAGGTGGCCCCAGAAGGGCAGGAGACAGTTGCAGTGC
  CAGACAGGGAGAGAGTAGAGACCCCTGGAGTGGGGAAGGAGGATGAGAAAGAAGAGCTGCAAGCTGAAAA
  GGTAACCAAGCAAGGGCTGTCTGCTGAGAAGGCACCCTCACTCTGTCCGGAGCTGACAACCGATGACAAT
  CGGCCCTTGAGCAGGCTGAGTAAAGCCAGCAGCAGGGCCAGGTCAGATGATCTCACCATA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218273 representing NM_008123
  Red=Cloning site Green=Tags(s)
  
  MGDWSFLGNILEEVNEHSTVIGRVWLTVLFIFRILILGTAAEFVWGDEQSDFVCNTQQPGCENVCYDEAF
  PISHIRLWVLQIIFVSTPSLMYVGHAVHHVRMEEKRKDREAEELCQQSRSNGGERVPIAPDQASIRKSSS
  SSKGTKKFRLEGTLLRTYVCHIIFKTLFEVGFIVGHYFLYGFRILPLYRCSRWPCPNVVDCFVSRPTEKT
  IFILFMLSVAFVSLFLNIMEMSHLGMKGIRSAFKRPVEQPLGEIAEKSLHSIAVSSIQKAKGYQLLEEEK
  IVSHYFPLTEVGMVETSPLSAKPFSQFEEKIGTGPLADMSRSYQETLPSYAQVGVQEVEREEPPIEEAVE
  PEVGEKKQEAEKVAPEGQETVAVPDRERVETPGVGKEDEKEELQAEKVTKQGLSAEKAPSLCPELTTDDN
  RPLSRLSKASSRARSDDLTI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008123
• ORF Size: 1320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008123.3, NP_032149.1
• RefSeq Size: 1394 bp
• RefSeq ORF: 1323 bp
• Locus ID: 14616
• UniProt ID: P28236
• Cytogenetics: 3
• Gene Summary: Structural component of eye lens gap junctions (PubMed:1325220). Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane (By similarity). Small molecules and ions diffuse from one cell to a neighboring cell via the central pore (PubMed:1325220).[UniProtKB/Swiss-Prot Function]