Gja8 (NM_008123) Mouse Untagged Clone

CAT#: MC215790

Gja8 (untagged) - Mouse gap junction protein, alpha 8 (Gja8), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gja8
• Synonyms: Aey5; Cnx50; Cx50; Lop10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215790 representing NM_008123
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGACTGGAGTTTCCTGGGAAACATCTTGGAAGAGGTGAATGAGCACTCCACTGTCATCGGCAGAG
  TCTGGCTCACAGTGCTCTTCATCTTCCGCATCCTCATCCTCGGGACAGCAGCGGAGTTTGTGTGGGGCGA
  TGAGCAATCTGATTTTGTATGCAACACCCAGCAGCCAGGCTGTGAGAATGTCTGCTACGATGAGGCCTTT
  CCCATCTCACACATCCGCCTCTGGGTGCTGCAGATCATCTTCGTCTCCACTCCATCGCTGATGTACGTGG
  GGCACGCGGTACACCACGTTCGCATGGAGGAGAAGCGAAAGGACCGTGAAGCTGAGGAGCTCTGTCAGCA
  GTCGCGCAGCAACGGGGGTGAGAGGGTACCAATCGCCCCAGACCAGGCCAGCATCCGGAAGAGCAGCAGC
  AGTAGCAAAGGCACCAAGAAGTTCCGGCTGGAGGGCACACTGCTAAGGACCTATGTCTGCCACATCATCT
  TCAAGACCCTCTTTGAGGTGGGCTTCATCGTGGGCCATTACTTCCTGTATGGTTTCCGCATCCTGCCCCT
  CTATCGCTGCAGCCGGTGGCCCTGCCCCAATGTGGTAGACTGCTTTGTATCCCGGCCTACTGAGAAGACC
  ATCTTCATCCTCTTCATGTTATCAGTCGCTTTTGTGTCACTCTTCCTCAACATCATGGAGATGAGCCACC
  TGGGCATGAAAGGAATCCGGTCTGCCTTCAAGAGGCCTGTAGAGCAACCACTGGGGGAGATTGCTGAGAA
  GTCCCTCCACTCCATTGCAGTTTCCTCCATCCAGAAAGCCAAGGGCTACCAGCTTCTAGAAGAAGAGAAG
  ATCGTATCACACTATTTCCCTTTGACAGAGGTTGGAATGGTGGAGACCAGCCCTCTTTCGGCCAAGCCTT
  TTAGTCAGTTTGAGGAGAAGATCGGCACAGGACCCCTGGCAGATATGTCACGGAGTTACCAAGAAACCCT
  GCCTTCTTATGCTCAGGTGGGGGTCCAGGAAGTGGAGCGGGAAGAGCCGCCTATAGAAGAGGCTGTGGAA
  CCGGAAGTGGGAGAGAAGAAGCAAGAAGCAGAGAAGGTGGCCCCAGAAGGGCAGGAGACAGTTGCAGTGC
  CAGACAGGGAGAGAGTAGAGACCCCTGGAGTGGGGAAGGAGGATGAGAAAGAAGAGCTGCAAGCTGAAAA
  GGTAACCAAGCAAGGGCTGTCTGCTGAGAAGGCACCCTCACTCTGTCCGGAGCTGACAACCGATGACAAT
  CGGCCCTTGAGCAGGCTGAGTAAAGCCAGCAGCAGGGCCAGGTCAGATGATCTCACCATATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008123
• Insert Size: 1323 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008123.3, NP_032149.1
• RefSeq Size: 6989 bp
• RefSeq ORF: 1323 bp
• Locus ID: 14616
• UniProt ID: P28236
• Cytogenetics: 3
• Gene Summary: Structural component of eye lens gap junctions (PubMed:1325220). Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane (By similarity). Small molecules and ions diffuse from one cell to a neighboring cell via the central pore (PubMed:1325220).[UniProtKB/Swiss-Prot Function]