Atp2c1 (NM_175025) Mouse Tagged ORF Clone

CAT#: MG218228

Atp2c1 (tGFP-tagged) - Mouse ATPase Ca++-sequestering (Atp2c1), (10ug)

Product Images

Circular map for MG218228

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Atp2c1
• Synonyms: 1700121J11Rik; ATP2C1A; AW061228; BCPM; D930003G21Rik; HHD; pmr1; SPCA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218228 representing NM_175025
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGTTGCACGATTTCAAAAGATCCCTAATGTTGAAAATGAGACAATGATCCCTGTACTGACGTCAA
  AGAGAGCAAGTGAGTTAGCAGTCAGTGAGGTTGCAGGCCTTCTCCAGGCTGATCTTCAGAATGGCTTAAA
  CAAATCTGAAGTTAGTCATAGGCGAGCCTTCCATGGTTGGAATGAATTTGATATCAGTGAAGATGAACCA
  TTATGGAAGAAGTATATTTCTCAGTTTAAAAATCCCCTTATCATGCTGCTCCTGGCGTCTGCGGTCATCA
  GCATTTTAATGCGCCAGTTTGATGATGCCGTCAGTATCACTGTGGCAATAGTAATTGTCGTCACTGTGGC
  CTTTGTTCAGGAATATCGTTCAGAAAAATCTCTAGAAGAATTGAGTAAACTTGTGCCACCAGAATGCCAT
  TGTGTGCGTGAAGGAAAACTGGAGCATACACTTGCCCGAGACTTGGTTCCAGGTGACACAGTTTGTCTCT
  CTGTGGGGGACAGAGTTCCTGCGGACTTACGCTTATTTGAGGCTGTGGATCTTTCTGTGGATGAGTCTAG
  CTTGACAGGAGAGACAGCTCCGTGCTCTAAAGTGACAGCCCCTCAGCCAGCTGCTAATGGAGATCTTGCG
  TCAAGAAGTAACATCGCCTTCATGGGGACACTGGTCAGATGTGGCAAAGCAAAGGGTATTGTCATTGGAA
  CAGGAGAAAATTCTGAATTTGGAGAGGTCTTTAAGATGATGCAGGCAGAAGAAGCACCAAAAACTCCTCT
  GCAGAAGAGCATGGACCTCTTAGGCAAGCAGCTGTCCTTTTACTCCTTTGGTATAATAGGTATCATCATG
  TTGGTTGGCTGGTTACTAGGAAAAGACATTCTGGAAATGTTCACTATTAGTGTAAGCTTGGCTGTAGCTG
  CAATTCCTGAAGGCCTGCCTATTGTGGTCACGGTGACTCTAGCCCTTGGTGTTATGAGAATGGTGAAGAA
  AAGGGCTATTGTAAAGAAATTGCCTATTGTGGAAACACTGGGCTGCTGTAATGTGATTTGTTCAGATAAA
  ACTGGAACCCTGACGAAGAACGAGATGACTGTTACTCACATCCTCACTTCCGATGGCCTGCATGCTGAGG
  TCACTGGAGTTGGCTATAATCAGTTTGGTGAAGTGATCGTTGATGGTGATGTTGTTCATGGATTTTATAA
  CCCAGCTGTTAGCAGAATTGTTGAGGCAGGCTGTGTGTGCAATGATGCTGTAATTAGGAACAACACTCTG
  ATGGGAAAGCCAACTGAAGGAGCCTTAATCGCTCTTGCTATGAAGATGGGTCTTGATGGACTGCAACAAG
  ACTACATCAGGAAAGCTGAATACCCTTTTAGTTCTGAGCAGAAGTGGATGGCTGTTAAGTGTGTGCACCG
  AACACAGCAGGACAGACCAGAGATTTGTTTTATGAAGGGTGCTTATGAGCAGGTGATTAAGTATTGTACT
  ACATACAACAGCAAAGGGCAGACTTTGGCACTTACCCAGCAGCAGAGAGATTTGTATCAACAAGAGAAGG
  CACGGATGGGCTCAGCGGGACTCAGAGTTCTTGCCTTGGCGTCTGGTCCGGAACTGGGGCAGCTGACCTT
  CCTTGGCCTGGTCGGAATCATTGACCCTCCTAGAACTGGTGTGAAGGAAGCTGTCACAACACTCATTGCC
  TCAGGAGTCTCCATCAAAATGATCACTGGAGATTCTCAGGAGACTGCAATTGCCATCGCTAGTCGTCTGG
  GATTGTACTCTAAGACTTCACAGTCCGTGTCTGGGGAAGAAGTCGATACAATGGAGGTGCAGCACCTTTC
  ACAGATAGTGCCAAAGGTTGCAGTATTTTACAGAGCAAGCCCAAGACACAAGATGAAAATTATTAAGTCT
  CTACAGAAGAACGGGGCAGTTGTAGCCATGACAGGAGATGGGGTAAATGATGCAGTTGCTCTCAAGGCTG
  CAGACATTGGAGTTGCGATGGGCCAGACTGGCACCGATGTTTGCAAAGAGGCTGCAGACATGATCTTGGT
  GGATGATGATTTCCAAACCATCATGTCTGCAATAGAAGAGGGTAAAGGCATTTATAATAACATTAAAAAT
  TTTGTTAGATTTCAACTGAGCACGAGTATAGCAGCATTAACTTTAATCTCATTGGCTACGTTAATGAACT
  TTCCTAACCCTCTCAATGCAATGCAGATTTTGTGGATCAATATTATAATGGATGGACCCCCAGCTCAGAG
  CCTTGGAGTAGAGCCAGTGGATAAAGATGTCATTCGAAAACCGCCTCGAAACTGGAAGGACAGCATTTTG
  ACAAAAAACTTGATACTTAAAATACTTGTTTCATCAATAATCATTGTTTGTGGGACTTTGTTTGTCTTCT
  GGCGAGAGCTTCGAGACAATGTGATAACACCCCGAGACACAACCATGACTTTCACTTGCTTTGTGTTTTT
  TGACATGTTCAATGCACTGAGTTCCAGATCTCAGACCAAGTCTGTGTTTGAGATTGGACTCTGCAGTAAT
  AAGATGTTCTGCTATGCAGTTCTTGGATCCATCATGGGACAGTTGCTTGTCATTTACTTCCCTCCTCTCC
  AGAAGGTTTTTCAAACCGAGAGCCTGAGTATACTGGATCTGTTGTTTCTTTTGGGCCTTACCTCGTCAGT
  GTGCATTGTGTCTGAGATTATAAAGAAGGTTGAAAGGAGCCGGGAGAAGGTCCAGAAGAATGCTGGTTCA
  GCATCATCGTCTTTCCTTGAAGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218228 representing NM_175025
  Red=Cloning site Green=Tags(s)
  
  MKVARFQKIPNVENETMIPVLTSKRASELAVSEVAGLLQADLQNGLNKSEVSHRRAFHGWNEFDISEDEP
  LWKKYISQFKNPLIMLLLASAVISILMRQFDDAVSITVAIVIVVTVAFVQEYRSEKSLEELSKLVPPECH
  CVREGKLEHTLARDLVPGDTVCLSVGDRVPADLRLFEAVDLSVDESSLTGETAPCSKVTAPQPAANGDLA
  SRSNIAFMGTLVRCGKAKGIVIGTGENSEFGEVFKMMQAEEAPKTPLQKSMDLLGKQLSFYSFGIIGIIM
  LVGWLLGKDILEMFTISVSLAVAAIPEGLPIVVTVTLALGVMRMVKKRAIVKKLPIVETLGCCNVICSDK
  TGTLTKNEMTVTHILTSDGLHAEVTGVGYNQFGEVIVDGDVVHGFYNPAVSRIVEAGCVCNDAVIRNNTL
  MGKPTEGALIALAMKMGLDGLQQDYIRKAEYPFSSEQKWMAVKCVHRTQQDRPEICFMKGAYEQVIKYCT
  TYNSKGQTLALTQQQRDLYQQEKARMGSAGLRVLALASGPELGQLTFLGLVGIIDPPRTGVKEAVTTLIA
  SGVSIKMITGDSQETAIAIASRLGLYSKTSQSVSGEEVDTMEVQHLSQIVPKVAVFYRASPRHKMKIIKS
  LQKNGAVVAMTGDGVNDAVALKAADIGVAMGQTGTDVCKEAADMILVDDDFQTIMSAIEEGKGIYNNIKN
  FVRFQLSTSIAALTLISLATLMNFPNPLNAMQILWINIIMDGPPAQSLGVEPVDKDVIRKPPRNWKDSIL
  TKNLILKILVSSIIIVCGTLFVFWRELRDNVITPRDTTMTFTCFVFFDMFNALSSRSQTKSVFEIGLCSN
  KMFCYAVLGSIMGQLLVIYFPPLQKVFQTESLSILDLLFLLGLTSSVCIVSEIIKKVERSREKVQKNAGS
  ASSSFLEV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175025
• ORF Size: 2754 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175025.4, NP_778190.3
• RefSeq Size: 4889 bp
• RefSeq ORF: 2757 bp
• Locus ID: 235574
• UniProt ID: Q80XR2
• Cytogenetics: 9 F1
• Gene Summary: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium.[UniProtKB/Swiss-Prot Function]