Hgsnat (NM_029884) Mouse Tagged ORF Clone

CAT#: MG218046

Hgsnat (tGFP-tagged) - Mouse heparan-alpha-glucosaminide N-acetyltransferase (Hgsnat), (10ug)

Product Images

Circular map for MG218046

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hgsnat
• Synonyms: 9430010M12Rik; AW208455; D8Ertd354e; Tmem76
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218046 representing NM_029884
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGGCGGGTCGAGCTCGCGCAGGCGCCGGGCAGAAGAGCGCAGCTCGGCTGCGGGGACAGAGCGCA
  ACTCCCGGAGAGAGGCTGTGGGAGGCATGGGCGCGGGCCCGGCGCTGGCAGCGCTGCTGCTGGCCGGGTC
  GGTGCTGAGCGCCACGCTGCTGGCCCCCGGCCGCCGGGCGGAGCCAGATTTAGATGAAAAAAGGAACGTG
  GAGCTGAAGATGGATCAGGCTTTGCTGCTTATTCACAATGAACTCTTGGGGACGAGCCTGACTGTGTACT
  GGAAATCTGATGACTGTTACCAATGCACCTTCCAGCCACTGGCGAACGTCTCTCACGGTGGAAAGCCAGC
  AAAGCCAAGCGTGGCACCTGTCTCTGTGAGCACCCAGCATGGGTCCATCCTGCAGGTTAACTCCACCTCG
  GAGGAGAGAGCAGCTTGCAGGCTGGAATACAAATTTGGAGAATTTGGAAACTATTCCCTTTTGGTGCAGC
  ATGCTTCCAGCGGGGCCAATAAAATTGCCTGTGACATCATCGTCAATGAGAATCCAGTGGACAGTAACCT
  TCCTGTGAGCATCGCATTCCTTGTTGGTTTAGCCCTCATCGTCGCAGTATCCCTCCTGAGGCTCTTGCTG
  AGTTTGGATGATGTCAATAACTGGATATCTAAGACAATAGCTTCTCGAGAAACTGACCGTCTCATCAATT
  CTGAGCTAGGGTCTCCCAGCAGAGCAGACCCTCTCAGCGCTGATTACCAACCAGAAACTCGGCGTTCTTC
  TGCGAACCGTCTCCGATGTGTGGACACATTTAGGGGGTTAGCTCTCGTCCTCATGGTCTTTGTTAACTAT
  GGAGGAGGAAAGTATTGGTACTTCAAACATTCAAGTTGGAACGGACTGACTGTGGCCGACCTCGTCTTCC
  CATGGTTTGTATTTATTATGGGAACTTCAATTTTCCTATCAATGACTTCTATCCTGCAACGAGGATGTTC
  AAAACTCAAACTGCTGGGGAAGATTGTGTGGAGAAGCTTCCTGCTAATCTGTATAGGAGTTATCATTGTC
  AACCCCAATTATTGCCTTGGTCCATTGTCTTGGGATAAAGTGCGGATCCCTGGTGTCCTGCAGCGGTTGG
  GAGTGACATACTTCGTGGTCGCTGTGTTGGAGTTCTTTTTCTGGAAGCCTGTGCCTGACAGTTGTACCTT
  GGAAAGCAGCTGCTTCTCTCTCCGAGACATCACGTCCAGCTGGCCCCAGTGGCTCACCATTCTGACGCTG
  GAAAGCATTTGGCTGGCTTTGACATTCTTTCTGCCTGTCCCTGGGTGCCCTACTGGCTACCTTGGCCCTG
  GCGGTATAGGGGACTTGGGGAAGTATCCTCATTGTACTGGAGGAGCAGCTGGCTACATCGACCGCCTGCT
  CCTGGGAGACAATCACCTGTACCAGCACCCATCCTCCACCGTGCTGTATCACACAGAGGTGGCCTACGAC
  CCTGAAGGAGTCTTAGGGACTATCAACTCAATTGTGATGGCGTTTTTAGGAGTTCAGGCAGGAAAAATAC
  TGGTGTATTACAAGGATCAGACGAAAGCCATCCTGACCCGATTTGCCGCCTGGTGCTGTATCCTTGGGCT
  TATTTCTATTGTGTTGACAAAAGTGTCTGCAAATGAAGGTTTTATTCCAATAAACAAGAATCTCTGGTCC
  ATCTCGTACGTCACCACACTGAGCTGCTTTGCCTTCTTCATCCTCCTCATTCTCTACCCAGTTGTGGATG
  TGAAGGGCCTGTGGACGGGAACCCCCTTCTTCTATCCAGGGATGAACTCCATCCTGGTGTATGTTGGCCA
  CGAGGTGTTGGAGAACTACTTCCCCTTCCAGTGGAAGCTGGCGGATGAGCAGTCACACAAAGAGCATCTC
  ATTCAGAACATAGTCGCCACGGCTCTCTGGGTGCTCATCGCCTACGTGCTCTATAAAAAGAAGCTGTTTT
  GGAAAATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218046 representing NM_029884
  Red=Cloning site Green=Tags(s)
  
  MTGGSSSRRRRAEERSSAAGTERNSRREAVGGMGAGPALAALLLAGSVLSATLLAPGRRAEPDLDEKRNV
  ELKMDQALLLIHNELLGTSLTVYWKSDDCYQCTFQPLANVSHGGKPAKPSVAPVSVSTQHGSILQVNSTS
  EERAACRLEYKFGEFGNYSLLVQHASSGANKIACDIIVNENPVDSNLPVSIAFLVGLALIVAVSLLRLLL
  SLDDVNNWISKTIASRETDRLINSELGSPSRADPLSADYQPETRRSSANRLRCVDTFRGLALVLMVFVNY
  GGGKYWYFKHSSWNGLTVADLVFPWFVFIMGTSIFLSMTSILQRGCSKLKLLGKIVWRSFLLICIGVIIV
  NPNYCLGPLSWDKVRIPGVLQRLGVTYFVVAVLEFFFWKPVPDSCTLESSCFSLRDITSSWPQWLTILTL
  ESIWLALTFFLPVPGCPTGYLGPGGIGDLGKYPHCTGGAAGYIDRLLLGDNHLYQHPSSTVLYHTEVAYD
  PEGVLGTINSIVMAFLGVQAGKILVYYKDQTKAILTRFAAWCCILGLISIVLTKVSANEGFIPINKNLWS
  ISYVTTLSCFAFFILLILYPVVDVKGLWTGTPFFYPGMNSILVYVGHEVLENYFPFQWKLADEQSHKEHL
  IQNIVATALWVLIAYVLYKKKLFWKI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029884
• ORF Size: 1968 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029884.1, NP_084160.1
• RefSeq Size: 2679 bp
• RefSeq ORF: 1971 bp
• Locus ID: 52120
• UniProt ID: Q3UDW8
• Cytogenetics: 8 14.22 cM
• Gene Summary: Lysosomal acetyltransferase that acetylates the non-reducing terminal alpha-glucosamine residue of intralysosomal heparin or heparan sulfate, converting it into a substrate for luminal alpha-N-acetyl glucosaminidase.[UniProtKB/Swiss-Prot Function]