Sytl1 (NM_031393) Mouse Tagged ORF Clone

CAT#: MG217979

Sytl1 (tGFP-tagged) - Mouse synaptotagmin-like 1 (Sytl1), (10ug)

Product Images

Circular map for MG217979

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sytl1
• Synonyms: 9030417L18; PSGL-1; Slp1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217979 representing NM_031393
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCAGAGAGGCCACCCATCTCAGGAGAGGCTTTGGGCCCTGCCCTCCCTCCCCATGGCACATGGAC
  CAGGATCTGAAGCTGAGGGGTTGCTGGACCTCAGCTTCCTGACAGAGGAGGAACAGGAGGCCATCTCTGA
  TGTCCTCAAACGAGATGCCCACCTGCGCCAGCTGGAGGAAGGGCGGGTCAGCAAGCTCCGGGCCTCCCTG
  GAAGACCCCTGGCAGCTGAAGATCCTGACAGGGGACTGGTTCCAAGAAGCGCGTTCCCAGAGGCACCATC
  ACGCCCATTTTGGTTCTGACCTCGTCCGGGCTTCTATCCGAAGGAAGAAGAGCCCAAAGGGGGACCAGGC
  TCTGGGGAGTGATGGTGAAGCAGAGGCTGCTGGGGAAAACACCATTGAAGGAGAGCCGGAGTCCAGGGTC
  TCCATAGAGGTGGCAGCTCCAGAGAGAAACACTGAGACCCAAGGGCCTGATTTGTTCTCACCATATGTAC
  CCTCAAAGGCTTCCGAAGGTCAGGAGGAGGAGCCCCAGGACCAGGAATGTGAGCTGGAGGCCCCTGGCGA
  AGGGGGTGTGCAGGTGGCAGAGGCGGACCCAGAGCTGGATCCTGAGCAGAAGGCGGAGCAGGAGTCCCAG
  CCAACTCCAGCCCAGTCCAAGGCGACCTCCAAGATCCTGGAGAATGGAGAAGAGGCCCCGGGGCTCGGCC
  CCTCTCTTGACCGCATGCTCAGCAGCAGCTCCTCTGTGTCCAGCCTCAATTCCTCTACGCTGAGCGGCAG
  CCTGATGAGCCTATCCGGGGAGGCAGAGGCAGGCACGGTGCAGGTGCGAGGCTCCGTGCTCTTCTCGCTG
  CGCTACGAGCCTGGCACCTCTGAGCTGCGAGTACAGGTGATCCAGTGCCAGGGCCTGGCCGCTGCCCGGC
  GACGCCGCTCCGATCCCTACGTGAAAAGCTACCTCCTCCCGGATAAGCAGAGCAAGCGTAAGACGTCAGT
  GAAGAAACGGAATCTGAACCCGATCTTCAACGAGACTCTGCGGCACTCTGTCCAGCAGGCTGATCTCCCA
  GGCCGCGTGCTGAGCCTGTCCGTGTGGCACCGGGAAAGTCTGGGTCGCAACATCTTCCTGGGAGAAGTAG
  AAGTGCCCCTAGACACATGGAACTGGGACTCTGAGGCTACCTGGCTCCCCCTGCAGCCTCGGGTTCCTCC
  ATCTCCAGATGAGCTTCCCAGCCGTGGACTGCTGTCTCTGTCCCTTAAGTATGTCCCTGCCGGCTCAGAG
  GGAGGAGGACAGCCTCAGAGTGGGGAGTTACACTTCTGGGTAAAGGAAGCTCAGAGCCTGGTACCACTGC
  GACCAGGATCCCTGGACACTTACATTCAATGCTCAGTGCTGCCTGATGACAGTCGGGCCAGCCGCCAACG
  CACAAGAGTGGTTCGAAGAAGTCTCAGCCCTGTGTTCAACCACACCATGGTTTATGACGGCTTCGGGCCT
  GCTGACCTACGCCAGGCCTGTGCCGAGCTCTCCCTGTGGGACCATGGGGCCCTGGCCAGTCGCCAGCTGG
  GGGGCACACGCCTCAGCCTTGGTACAGGCAGCAGTTATGGGCTTCAGGTGCCCTGGATGGATTCCACACC
  GGAGGAGAAGCAGCTGTGGCAGACACTCCTGGAGCGGCCCTGTGAGTGGGTGGATGGCCTTCTGCCCCTC
  AGAACCAACCTGGTCCCCAGGGCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217979 representing NM_031393
  Red=Cloning site Green=Tags(s)
  
  MPQRGHPSQERLWALPSLPMAHGPGSEAEGLLDLSFLTEEEQEAISDVLKRDAHLRQLEEGRVSKLRASL
  EDPWQLKILTGDWFQEARSQRHHHAHFGSDLVRASIRRKKSPKGDQALGSDGEAEAAGENTIEGEPESRV
  SIEVAAPERNTETQGPDLFSPYVPSKASEGQEEEPQDQECELEAPGEGGVQVAEADPELDPEQKAEQESQ
  PTPAQSKATSKILENGEEAPGLGPSLDRMLSSSSSVSSLNSSTLSGSLMSLSGEAEAGTVQVRGSVLFSL
  RYEPGTSELRVQVIQCQGLAAARRRRSDPYVKSYLLPDKQSKRKTSVKKRNLNPIFNETLRHSVQQADLP
  GRVLSLSVWHRESLGRNIFLGEVEVPLDTWNWDSEATWLPLQPRVPPSPDELPSRGLLSLSLKYVPAGSE
  GGGQPQSGELHFWVKEAQSLVPLRPGSLDTYIQCSVLPDDSRASRQRTRVVRRSLSPVFNHTMVYDGFGP
  ADLRQACAELSLWDHGALASRQLGGTRLSLGTGSSYGLQVPWMDSTPEEKQLWQTLLERPCEWVDGLLPL
  RTNLVPRA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031393
• ORF Size: 1704 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031393.2, NP_113570.2
• RefSeq Size: 1940 bp
• RefSeq ORF: 1707 bp
• Locus ID: 269589
• Cytogenetics: 4 D2.3