Nckap1 (NM_016965) Mouse Tagged ORF Clone

CAT#: MG217978

Nckap1 (tGFP-tagged) - Mouse NCK-associated protein 1 (Nckap1), (10ug)

Product Images

Circular map for MG217978

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nckap1
• Synonyms: C79304; H19; Hem-2; Hem2; mh19; mKIAA0587; Nap1; p125Nap1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217978 representing NM_016965
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCGCTCCGTGCTGCAGCCCAGTCAGCAGAAGCTGGCGGAGAAGCTCACCATCCTCAACGACCGGG
  GCGTCGGCATGCTCACGCGCCTCTACAACATCAAGAAGGCATGTGGCGACCCCAAGGCTAAACCCTCCTA
  CCTTATTGACAAAAACCTGGAATCTGCTGTGAAATTCATAGTCAGAAAATTTCCTGCTGTAGAAACGCGC
  AACAACAATCAACAGCTGGCACAGCTACAGAAAGAAAAATCAGAGATTCTGAAAAATCTGGCGTTGTATT
  ACTTTACATTTGTAGATGTTATGGAATTTAAGGACCATGTCTGTGACTTGCTGAATACTATTGACGTTTG
  CCAAGTCTTCTTTGATATTACTGTGAACTTTGATTTAACAAAGAACTACTTAGACTTGACTGTAACCTAT
  ACAACATTAATGATACTGCTGTCCCGGATTGAAGAACGGAAGGCAATCATTGGACTATACAATTATGCAC
  ATGAAATGACCCATGGAGCAAGTGACCGAGAATATCCACGCCTTGGTCAGATGATTGTGGACTATGAAAA
  CCCTTTAAAAAAGATGATGGAAGAATTTGTACCCCATAGCAAGTCTCTTTCAGATGCGCTAATTTCTCTT
  CAGATGGTGTATCCTAGACGGAACCTTTCAGCAGACCAGTGGAGGAATGCCCAGTTGCTGAGTCTCATCA
  GTGCACCCAGCACAATGCTTAATCCTGCGCAGTCGGACACTATGCCTTGTGAATATCTCTCCTTGGATGC
  AATGGAGAAATGGATCATCTTTGGCTTTATTCTGTGCCATGGGATGCTAAATACGGAGGCTACCGCACTG
  AACCTTTGGAAGCTAGCTCTTCAGAGTAGCTCTTGCCTCTCTCTCTTTCGGGATGAAGTTTTCCACATTC
  ACAAAGCTGCAGAAGACTTATTTGTAAACATTCGGGGCTACAATAAACGTATTAACGACATAAGAGAATG
  CAAGGAGGCGGCTGTATCCCACGCCGGCTCAATGCACAGAGAAAGACGCAAGTTTTTACGGTCTGCACTA
  AAAGAACTGGCCACTGTCCTCTCTGACCAGCCTGGCCTGCTTGGTCCCAAGGCACTTTTTGTTTTCATGG
  CGTTATCTTTTGCCCGTGATGAAATTATCTGGCTGCTCCGTCACGCGGACAACATGCCAAAGAAGAGTGC
  AGATGACTTTATAGATAAGCACATTGCTGAGCTAATATTTTACATGGAAGAGCTTAGAGCACATGTCAGG
  AAGTATGGGCCTGTCATGCAGAGGTACTACGTGCAATACCTCTCTGGCTTTGATGCTGTTGTCCTCAATG
  AACTTGTGCAGAACCTGTCTGTTTGCCCTGAGGATGAATCGATCATCATGTCCTCTTTTGTGAACACCAT
  GACCTCCCTAAGTGTGAAGCAAGTTGAAGATGGAGAAGTGTTTGATTTCAGAGGGATGAGATTAGATTGG
  TTTAGGTTACAGGCGTACACTAGCGTCTCCAAAGCTTCACTTAGCCTTGCAGATCACAGAGAACTTGGAA
  AGATGATGAACACAATCATATTCCATACAAAAATGGTAGATTCCTTGGTTGAAATGTTGGTGGAAACATC
  CGATCTCTCCATATTTTGTTTTTACAGCCGTGCTTTTGAGAAGATGTTTCAGCAGTGCTTGGAGCTGCCC
  TCTCAGTCAAGATACTCCATTGCCTTTCCTCTGCTCTGCACTCACTTCATGAGCTGCACACATGAACTGT
  GTCCTGAAGAGCGCCATCATATAGGAGATCGTAGCCTTTCCCTGTGCAATATGTTCCTGGATGAGATGGC
  CAAACAAGCTCGAAATCTCATCACCGATATTTGCACAGAACAGTGTACTCTTAGTGACCAGTTATTGCCG
  AAGCATTGTGCCAAAACCATCAGTCAAGCAGTGAATAAGAAGTCAAAAAAACAGACGGGCAAGAAAGGGG
  AACCTGAAAGGGAAAAACCGGGTGTGGAGAGCATGAGGAAAAACAGGCTGGTAGTGACCAACCTTGATAA
  GTTGCACACTGCACTTTCGGAGTTATGCTTCTCCATAAATTATGTTCCAAACATGGCGGTGTGGGAGCAC
  ACCTTCACGCCGAGGGAGTATTTGACTTCTCATCTGGAAATCCGGTTCACTAAATCAATTGTTGGAATGA
  CTATGTATAATCAAGCTACACAGGAAATTGCAAAGCCATCAGAGCTTCTAACAAGCGTAAGAGCATATAT
  GACTGTACTCCAGTCCATAGAGAACTATGTGCAGATTGATATCACCAGAGTATTTAATAATGTCCTTCTT
  CAGCAAACACAACACTTAGACAGCCATGGAGAACCAACCATCACGAGTCTGTATACAAACTGGTACTTGG
  AAACTTTATTAAGACAAGTCAGCAATGGCCATATAGCTTATTTTCCTGCAATGAAAGCATTTGTGAATTT
  ACCCACAGAAAATGAATTAACATTCAATGCAGAGGAATATTCTGATATATCAGAAATGAGATCATTATCA
  GAGCTCCTAGGCCCATATGGGATGAAGTTCCTGAGTGAGAGCCTTATGTGGCACATTTCATCACAAGTAG
  CTGAACTTAAGAAACTTGTGGTGGAGAATGTTGATGTTCTAACACAAATGAGGACCAGCTTTGACAAACC
  AGACCAGATGGCTGCACTCTTTAAAAGATTATCATCTGTTGACAGTGTCTTGAAAAGGATGACGATAATT
  GGTGTTATTTTATCCTTCCGGTCATTGGCACAAGAAGCATTGAGAGATGTCCTGTCCTACCACATTCCTT
  TCCTCGTGAGCTCCATTGAAGATTTCAAGGATCACATTCCTCGGGAAACTGATATGAAGGTTGCAATGAA
  TGTGTATGAGCTGTCCTCGGCTGCTGGGTTACCATGTGAGATTGATCCTGCCTTAGTCGTAGCTCTTTCC
  TCACAAAAATCAGAGAACATTAGTCCAGAGGAAGAATACAAGATCGCTTGTCTCCTGATGGTCTTTGTAG
  CAGTTTCCTTGCCTACACTGGCCAGCAATGTAATGTCACAGTACAGCCCTGCAATAGAAGGACACTGCAA
  CAATATACATTGTTTGGCCAAAGCCATCAACCAGATTGCTGCAGCTTTGTTTACAATTCACAAAGGAAGT
  ATTGAAGACCGACTTAAGGAATTCCTGGCGCTTGCATCATCCAGTCTACTGAAAATTGGCCAAGAGACAG
  ATAAAACTACAACAAGAAATAGAGAATCTGTTTATTTGCTACTGGATATGATTGTACAAGAATCACCATT
  CCTGACAATGGATCTCTTGGAATCTTGTTTTCCTTATGTGTTGCTGAGAAATGCATACCATGCTGTCTAC
  AAACAAAGTGTTACATCTTCTGCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217978 representing NM_016965
  Red=Cloning site Green=Tags(s)
  
  MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETR
  NNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCDLLNTIDVCQVFFDITVNFDLTKNYLDLTVTY
  TTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISL
  QMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGMLNTEATAL
  NLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSAL
  KELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVR
  KYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDW
  FRLQAYTSVSKASLSLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELP
  SQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLP
  KHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMAVWEH
  TFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLL
  QQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLS
  ELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTII
  GVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALS
  SQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGS
  IEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVY
  KQSVTSSA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016965
• ORF Size: 3384 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016965.3, NP_058661.1
• RefSeq Size: 3387 bp
• RefSeq ORF: 3387 bp
• Locus ID: 50884
• UniProt ID: P28660
• Cytogenetics: 2 48.21 cM
• Gene Summary: Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (PubMed:27605705).[UniProtKB/Swiss-Prot Function]