Bora (NM_175265) Mouse Tagged ORF Clone

CAT#: MG217899

Bora (tGFP-tagged) - Mouse RIKEN cDNA 6720463M24 gene (6720463M24Rik), (10ug)

Product Images

Circular map for MG217899

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Bora
• Synonyms: 6720463M24Rik; AI317232
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217899 representing NM_175265
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGATGTCAGCGAATTAAAAATGCAAATAACACCAGAAACTCCAGGAAGGATCCCTGTTTTAAATC
  CCTTTGAAAGTCCCAGTGATTATTCTAATCTCCATGAACAAACCCTTGCTAGTCCTTCTATCTTTAAGTC
  CACAAAACTACCAACTCCAGGGAAATTTAGGTGGTCTATTGATCAGCTGGCCATAATAAACCCTGTAGAG
  ATCGATCCAGAGGAGATCCATCGGCAGGCTTCCTACCTAAGGCTTTCTCGAATAGATAAAGATGTGGAAG
  ACAAAAGACAAAAAGCCATTGAAGAGTTTTTTACTAAAGATGTCATTGTGCCCTCTCCCTGGACTGATCA
  TGATGGAAAACAGCCTTCAGAGCTTCATCCCAGTAAATGCCTAAGTAGCCATGATGACTCTCCTGATGGG
  AAAAAGCCAAGTCTTCCCTCACAGAAGTGCAACGCTGCCTGCCAGACTCTGCTGTCTCTTCCTGTGGATT
  TTAATTTGGAAGCCATATTAGGAGACTATTTTAGAGAAGAGGACTTTGTGGCCCACACTCCTGGGAACCT
  CAGTTCTTCATCTCTGAGAAGAAAGCTCTTTTTGGATGGGAATGGAAGCATCTGTGACCCTTTGCCTTCG
  CCGTCGCCCGGAAGTCCTCCCTGCAGTGCCCGGGGGTCGCTCGAGGGACAGTTTTCTTCCAGCCCCATTC
  AGAACAGTGTGAAAAAATACAGCTTGGGAAGCGTCACCACGAGCCCGTCGGCCATCTCCTCACCTACCTT
  CTCACCTATCGCACTTCAAGGAGGAAAGACGCCGCTCTCAGAACCCAGGAAGCTTACGTTTCATTCTCCC
  GAAGCTTCGTGTGCAACAGCTTCTACGGGGATCGTTAACCCGAGCATCAGGAGTCCGTACATAGATGGCT
  GCTCGCCAATCAAAAACTGGTCTCCTCGGAGACTGAGAGGTGGCCCTCAGTGTTTGTCCTCCCTGGTCCG
  CATACCTTTTACCCTTGAGGCTCACAGTGAAGATGAAGAGGCGGACGTTTCCTGCACAGGCGCGGCTCCA
  CTCAGCACAAATGCTTGTGGTGAGCCTCGAGTTGTCACTGCCATGTCTGTTACACAGAGTCACTCTGGTA
  TTGCTGAGAAAGAGCGAGCAGTGCTGGACGATGCGGAAAGTGAGAGGGAAAATGACACCGTGGATATGGT
  CGATCCTACAGACACAGTAGCTGAAAGCACGTGGATAAAAGAGCCAGTGGATGATGGGAATTCACCCATG
  ACGGATTCCGCGAGTGGAATTGCCTTCAGTATTGAAAACTCTCATATGTGCATGTCGCCTCTTGCAGAAA
  GCAGTGTCCTGCCTTATGAAAGCAGTGCTATCCAGATGAATAGTGACTATAACACACAGACTTGTGTAAG
  TAATATCACTGATATTGTTGGGACAGAAAGATATTGCAAAGAAAATGTCACACATACAAATGTTCCAGTG
  CCCTTTGAAGTGGAAATGAAATCTCAAGTTAATAACGTAACGCCCGGCCACACAGCGCAGCGGTGCTGGA
  TGAAATCTCCGCGTCCATCTCAGTGCAGCCGCCCG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217899 representing NM_175265
  Red=Cloning site Green=Tags(s)
  
  MGDVSELKMQITPETPGRIPVLNPFESPSDYSNLHEQTLASPSIFKSTKLPTPGKFRWSIDQLAIINPVE
  IDPEEIHRQASYLRLSRIDKDVEDKRQKAIEEFFTKDVIVPSPWTDHDGKQPSELHPSKCLSSHDDSPDG
  KKPSLPSQKCNAACQTLLSLPVDFNLEAILGDYFREEDFVAHTPGNLSSSSLRRKLFLDGNGSICDPLPS
  PSPGSPPCSARGSLEGQFSSSPIQNSVKKYSLGSVTTSPSAISSPTFSPIALQGGKTPLSEPRKLTFHSP
  EASCATASTGIVNPSIRSPYIDGCSPIKNWSPRRLRGGPQCLSSLVRIPFTLEAHSEDEEADVSCTGAAP
  LSTNACGEPRVVTAMSVTQSHSGIAEKERAVLDDAESERENDTVDMVDPTDTVAESTWIKEPVDDGNSPM
  TDSASGIAFSIENSHMCMSPLAESSVLPYESSAIQMNSDYNTQTCVSNITDIVGTERYCKENVTHTNVPV
  PFEVEMKSQVNNVTPGHTAQRCWMKSPRPSQCSRP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175265
• ORF Size: 1575 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175265.5
• RefSeq Size: 2293 bp
• RefSeq ORF: 1578 bp
• Locus ID: 77744
• UniProt ID: Q8BS90
• Cytogenetics: 14 E2.2
• Gene Summary: Required for the activation of AURKA at the onset of mitosis.[UniProtKB/Swiss-Prot Function]