Sptbn1 (NM_009260) Mouse Tagged ORF Clone

CAT#: MG217862

Sptbn1 (tGFP-tagged) - Mouse spectrin beta 2 (Spnb2) transcript variant 2, (10ug)

Product Images

Circular map for MG217862

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sptbn1
• Synonyms: 9930031C03Rik; AL033301; elf1; elf3; mKIAA4049; Spnb-2; Spnb2; SPTB2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217862 representing NM_009260
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTTGCAGAGGACATCCAGCATTTCAGGGCCGCTGTCGCCGGCCTACACCGGGCAGGTGCCTTACA
  ACTACAACCAACTGGAAGGAAGATTCAAACAGCTCCAAGATGAGCGTGAAGCTGTACAGAAGAAGACCTT
  CACCAAGTGGGTCAATTCCCACCTTGCGAGAGTGTCCTGCCGAATCACAGACCTGTACACGGACCTTCGA
  GATGGACGGATGCTCATCAAGCTACTGGAGGTCCTCTCTGGAGAGAGGCTGCCTAAACCCACTAAGGGAC
  GGATGCGGATCCACTGTCTGGAGAATGTCGACAAGGCTCTTCAATTCCTGAAAGAGCAGAGAGTCCATCT
  TGAGAACATGGGCTCCCATGACATTGTGGATGGAAACCACCGGCTGACCCTCGGCCTCATCTGGACAATT
  ATTCTGCGCTTCCAGATCCAGGATATTAGTGTGGAGACTGAAGATAACAAAGAGAAAAAGTCTGCTAAGG
  ATGCATTGCTGCTGTGGTGCCAGATGAAGACAGCTGGGTACCCCAATGTCAACATTCACAATTTCACCAC
  TAGCTGGAGGGATGGCATGGCCTTCAATGCACTGATACATAAACATCGGCCTGACCTGATAGATTTTGAT
  AAACTGAAGAAATCTAATGCACACTACAATCTGCAGAATGCATTTAACCTGGCAGAGCAGCACCTTGGCC
  TCACTAAACTGTTAGACCCTGAAGATATCAGTGTGGACCACCCTGATGAGAAGTCTATCATCACATACGT
  GGTGACTTACTACCACTACTTCTCCAAGATGAAGGCCTTGGCTGTCGAAGGAAAGCGCATTGGAAAGGTG
  CTTGATAATGCTATAGAAACAGAGAAAATGATTGAGAAGTACGAGTCACTTGCTTCTGACCTTCTGGAGT
  GGATTGAACAAACCATCATCATCCTAAACAACCGCAAATTTGCTAATTCACTGGTTGGGGTCCAGCAGCA
  GCTCCAGGCATTCAACACGTACCGCACAGTGGAGAAACCACCTAAGTTTACTGAGAAGGGGAATTTGGAG
  GTGCTCCTTTTCACAATTCAGAGCAAGATGCGAGCGAATAATCAGAAGGTCTACATGCCCCGCGAGGGGA
  AGCTCATCTCTGACATCAACAAGGCCTGGGAAAGACTGGAAAAAGCAGAACATGAGAGAGAACTGGCTCT
  GCGGAATGAGCTCATACGGCAGGAAAAACTGGAACAACTCGCCCGAAGATTTGATCGCAAGGCAGCTATG
  AGGGAGACATGGCTGAGTGAAAACCAGCGTCTTGTGTCTCAGGACAACTTTGGATTTGACCTTCCCGCTG
  TTGAGGCTGCTACCAAAAAACACGAGGCCATTGAGACAGACATCGCTGCATATGAAGAACGAGTTCAGGC
  CGTGGTGGCTGTGGCCAGGGAACTTGAAGCCGAGAACTACCATGACATCAAGCGCATCACAGCGAGGAAG
  GACAATGTCATCCGGCTCTGGGAATACTTGCTGGAACTGCTCAGGGCCAGGAGGCAGCGTCTTGAGATGA
  ACCTGGGATTGCAAAAGATATTCCAGGAAATGCTTTATATTATGGACTGGATGGATGAAATGAAGGTGCT
  ATTGCTGTCTCAAGACTATGGCAAACACTTACTTGGTGTTGAAGACCTGTTACAGAAGCATGCCCTGGTT
  GAAGCAGACATTGCAATCCAAGCAGAGCGTGTAAGAGGTGTGAATGCCTCTGCCCAGAAGTTTGCAACAG
  ATGGGGAAGGCTACAAGCCATGTGACCCCCAGGTAATTCGAGACCGTGTTGCCCACATGGAGTTCTGCTA
  TCAAGAGCTTTGTCAGCTGGCTGCCGAGCGTAGGGCTCGCCTGGAAGAGTCCCGTCGCCTCTGGAAGTTC
  TTCTGGGAGATGGCAGAAGAGGAAGGCTGGATACGAGAGAAGGAGAAGATCCTGTCCTCTGATGATTACG
  GGAAAGACTTGACCAGTGTCATGCGCCTGCTGAGCAAGCACCGGGCATTTGAGGATGAGATGAGTGGCCG
  TAGTGGCCATTTTGAGCAGGCCATTAAAGAAGGTGAAGACATGATTGCAGAGGAACACTTTGGATCGGAA
  AAGATCCGTGAGAGAATCATTTATATCCGGGAGCAGTGGGCCAACCTGGAACAGCTCTCAGCCATTAGGA
  AGAAGCGCCTAGAGGAAGCCTCATTACTGCACCAGTTCCAGGCTGATGCTGATGATATTGATGCTTGGAT
  GTTAGATATACTCAAGATTGTCTCCAGCAATGATGTGGGCCATGATGAGTACTCCACGCAGTCTCTGGTC
  AAGAAGCATAAAGATGTAGCAGAAGAGATCACCAACTACAGGCCCACTATTGACACACTGCATGAGCAAG
  CCAGTGCCCTTCCACAAGCACATGCAGAGTCTCCAGATGTGAAGGGCCGGCTGGCAGGAATTGAGGAGCG
  CTGCAAGGAGATGGCAGAGTTAACACGGCTAAGGAAGCAGGCTCTGCAGGACACCCTGGCCCTGTACAAG
  ATGTTCAGTGAGGCTGATGCCTGTGAGCTCTGGATTGACGAGAAGGAGCAGTGGCTCAACAACATGCAGA
  TCCCAGAGAAGCTGGAGGACCTGGAAGTCATCCAGCACAGATTTGAGAGCCTAGAACCAGAAATGAACAA
  CCAGGCTTCCCGGGTTGCTGTGGTGAACCAGATTGCACGGCAGCTGATGCACAATGGCCACCCCAGTGAA
  AAGGAAATCAGAGCTCAGCAAGACAAACTCAACACGAGGTGGAGTCAGTTCAGAGAACTGGTGGACAGGA
  AAAAGGATGCTCTTCTGTCTGCCCTGAGCATCCAGAACTACCACCTCGAGTGCAATGAAACCAAATCCTG
  GATCCGGGAGAAGACCAAGGTCATCGAGTCTACCCAAGACCTTGGCAATGACCTGGCAGGTGTCATGGCC
  CTGCAGCGCAAGCTGACTGGCATGGAACGAGACTTGGTAGCCATTGAGGCGAAGCTGAGTGACCTGCAGA
  AAGAAGCTGAGAAGCTGGAGTCCGAGCACCCTGACCAGGCTCAAGCTATCCTGTCTCGGCTGGCCGAGAT
  CAGTGATGTGTGGGAGGAAATGAAGACAACCCTGAAGAACCGAGAGGCCTCCCTGGGAGAGGCCAGCAAG
  CTGCAGCAGTTTCTGCGGGACTTGGACGACTTCCAGTCTTGGCTCTCCAGGACCCAGACTGCTATCGCCT
  CAGAGGACATGCCCAATACCCTCACTGAGGCAGAGAAGCTTCTCACACAGCACGAGAATATCAAAAATGA
  GATCGACAATTATGAGGAAGACTACCAGAAGATGCGGGACATGGGCGAGATGGTCACCCAGGGGCAGACT
  GATGCCCAGTATATGTTTCTGCGGCAGCGGCTGCAGGCCTTAGACACTGGCTGGAATGAGCTCCACAAAA
  TGTGGGAGAACAGGCAAAACCTCCTCTCCCAGTCCCATGCCTACCAGCAGTTCCTTAGGGACACCAAACA
  AGCTGAAGCTTTTCTTAATAACCAGGAGTATGTTTTGGCTCATACTGAAATGCCCACCACCCTGGAAGGA
  GCTGAAGCAGCCATTAAAAAGCAGGAGGACTTCATGACCACCATGGATGCCAACGAGGAGAAGATCAATG
  CTGTTGTGGAGACTGGCCGAAGACTGGTGAGCGATGGGAACATCAACTCCGACCGCATCCAGGAGAAGGT
  GGACTCTATTGACGACAGACACAGGAAGAATCGAGAAGCAGCCAGTGAACTTCTGATGAGGTTAAAGGAC
  AACCGTGATCTACAGAAGTTCCTGCAAGATTGTCAAGAGCTGTCCCTCTGGATCAATGAAAAGATGCTTA
  CAGCTCAAGACATGTCTTATGATGAAGCCAGAAATCTGCACAGTAAATGGTTAAAGCATCAAGCATTTAT
  GGCGGAACTTGCATCCAACAAAGAATGGCTTGACAAAATTGAGAAGGAAGGAATGCAGCTTATTTCAGAA
  AAGCCAGAAACAGAAGCTGTGGTAAAGGAAAAACTCACTGGTTTACATAAAATGTGGGAAGTCCTTGAAT
  CCACAACCCAGACCAAGGCCCAGCGGCTCTTTGATGCAAATAAGGCTGAGCTTTTCACACAAAGCTGCGC
  AGATCTTGACAAATGGCTACATGGCCTGGAGAGCCAGATTCAATCTGACGACTATGGCAAAGACCTTACC
  AGTGTCAATATTCTTCTGAAAAAGCAACAGATGCTGGAGAATCAGATGGAAGTTCGGAAGAAAGAGATCG
  AGGAACTGCAGAGCCAAGCCCAGGCGCTGAGTCAGGAGGGGAAGAGCACAGATGAGGTGGACAGCAAACG
  CCTTACTGTGCAGACCAAGTTCATGGAGCTTCTGGAGCCCTTGAGTGAGAGGAAGCATAACCTGTTAGCT
  TCCAAGGAGATCCATCAGTTCAACAGGGATGTGGAGGACGAAATCCTATGGGTTGGCGAGAGGATGCCTT
  TGGCAACTTCCACAGATCATGGCCATAACCTTCAAACTGTGCAGCTGTTAATAAAGAAAAACCAGACCCT
  CCAGAAAGAAATCCAGGGACACCAGCCTCGTATTGATGACATCTTTGAGAGGAGTCAAAACATCATCACA
  GATAGCAGCAGCCTCAATGCCGAGGCTATCAGGCAGAGGCTCGCTGACCTGAAGCAGCTGTGGGGGCTCC
  TCATTGAGGAAACTGAGAAACGCCATAGACGGCTGGAGGAGGCACACAAGGCGCAGCAGTACTACTTTGA
  TGCAGCTGAAGCCGAGGCATGGATGAGTGAACAGGAGTTGTACATGATGTCTGAGGAAAAGGCCAAGGAT
  GAGCAGAGTGCTGTCTCTATGTTGAAAAAGCACCAGATTTTAGAGCAAGCTGTTGAGGACTATGCAGAGA
  CAGTACACCAGCTCTCCAAGACTAGCCGGGCGCTGGTGGCTGACAGCCATCCCGAAAGTGAGCGTATTAG
  CATGCGGCAGTCAAAGGTCGACAAGCTGTATGCTGGCCTGAAGGACCTTGCTGAGGAGAGGAGAGGAAAA
  CTTGATGAGAGGCACAGGCTGTTCCAGCTCAACAGAGAGGTGGATGACCTGGAACAGTGGATCGCTGAGA
  GGGAAGTGGTCGCAGGCTCCCATGAGTTGGGACAGGACTATGAGCATGTCACGATGTTACAAGAACGGTT
  CCGAGAATTTGCTCGAGACACAGGAAACATTGGGCAGGAGCGTGTGGATACAGTTAATAACATGGCAGAT
  GAACTCATCAACTCTGGACATTCAGATGCTGCCACCATTGCTGAGTGGAAAGATGGTCTCAATGAAGCCT
  GGGCTGACCTCCTGGAGCTCATTGACACAAGAACACAGATTCTTGCTGCCTCATATGAACTTCATAAGTT
  TTACCATGATGCCAAGGAGATCTTTGGCCGAATCCAGGACAAACACAAGAAACTCCCTGAGGAGCTTGGA
  AGAGATCAAAACACTGTGGAAACTTTACAGAGAATGCACACCACCTTTGAGCACGACATCCAAGCTCTGG
  GCACTCAGGTGAGGCAGCTGCAGGAGGATGCAGCTCGCCTCCAGGCAGCCTATGCAGGGGACAAGGCTGA
  TGACATCCAGAAGCGTGAGAATGAGGTCCTGGAAGCCTGGAAGTCCCTGCTGGATGCTTGTGAGGGTCGC
  AGGGTGCGGCTGGTAGACACAGGAGACAAGTTCCGCTTCTTCAGCATGGTGCGTGACCTCATGCTCTGGA
  TGGAAGATGTCATCCGGCAGATCGAGGCCCAGGAGAAACCACGGGATGTGTCATCTGTTGAACTGTTAAT
  GAATAATCATCAAGGTATCAAAGCTGAAATTGATGCTCGTAATGACAGCTTTACAGCCTGCATTGAGCTT
  GGGAAATCCCTGCTGGCACGGAAACACTATGCTTCTGAGGAGATCAAGGAAAAGTTACTGCAGCTGACAG
  AGAAAAGAAAAGAAATGATTGACAAGTGGGAAGACCGGTGGGAGTGGTTAAGACTGATTTTGGAGGTCCA
  TCAGTTCTCAAGGGATGCCAGTGTGGCAGAGGCTTGGCTGCTTGGACAGGAACCATACCTATCCAGCCGT
  GAAATTGGCCAGAGTGTAGACGAAGTGGAGAAGCTTATTAAGCGCCATGAGGCGTTTGAAAAGTCTGCAG
  CGACCTGGGATGAGAGATTCTCTGCTCTGGAAAGGCTGACAACGTTGGAGCTACTGGAAGTGCGCAGACA
  GCAAGAGGAAGAAGAAAGAAAGAGGCGGCCACCTTCTCCGGACCCAAACACGAAGGTTTCAGAGGAGGCT
  GAGTCCCAGCAATGGGATACTTCAAAAGGAGACCAAGTTTCCCAGAATGGTTTGCCGGCTGAGCAGGGAT
  CTCCACGGGTTAGTTACCGCTCTCAAACGTACCAAAACTACAAAAACTTTAATAGCAGACGGACAGCCAG
  TGACCATTCATGGTCTGGAATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217862 representing NM_009260
  Red=Cloning site Green=Tags(s)
  
  MELQRTSSISGPLSPAYTGQVPYNYNQLEGRFKQLQDEREAVQKKTFTKWVNSHLARVSCRITDLYTDLR
  DGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTI
  ILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFD
  KLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKV
  LDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLE
  VLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAM
  RETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEAENYHDIKRITARK
  DNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALV
  EADIAIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAERRARLEESRRLWKF
  FWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGSE
  KIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLV
  KKHKDVAEEITNYRPTIDTLHEQASALPQAHAESPDVKGRLAGIEERCKEMAELTRLRKQALQDTLALYK
  MFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHNGHPSE
  KEIRAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMA
  LQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTTLKNREASLGEASK
  LQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQKMRDMGEMVTQGQT
  DAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEYVLAHTEMPTTLEG
  AEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKD
  NRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEKEGMQLISE
  KPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLESQIQSDDYGKDLT
  SVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLSERKHNLLA
  SKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIIT
  DSSSLNAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHKAQQYYFDAAEAEAWMSEQELYMMSEEKAKD
  EQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLYAGLKDLAEERRGK
  LDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNNMAD
  ELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELG
  RDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKSLLDACEGR
  RVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTACIEL
  GKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSR
  EIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRPPSPDPNTKVSEEA
  ESQQWDTSKGDQVSQNGLPAEQGSPRVSYRSQTYQNYKNFNSRRTASDHSWSGM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009260
• ORF Size: 6462 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009260.2, NP_033286.2
• RefSeq Size: 9049 bp
• RefSeq ORF: 6465 bp
• Locus ID: 20742
• UniProt ID: Q62261
• Cytogenetics: 11 17.44 cM
• Gene Summary: Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.[UniProtKB/Swiss-Prot Function]